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HAO1 Antibody

  • 货号：
    CSB-PA891938LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA891938LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA891938LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) HAO1 Polyclonal antibody
  • Uniprot No.：
    Q9UJM8
  • 基因名：
    HAO1
  • 别名：
    (S) 2 hydroxy acid oxidase antibody; Glycolate oxidase antibody; GOX antibody; GOX1 antibody; HAO1 antibody; HAOX1 antibody; HAOX1_HUMAN antibody; Hydroxyacid oxidase 1 antibody; MGC142225 antibody; MGC142227 antibody; OTTHUMP00000030231 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Hydroxyacid oxidase 1 protein (161-273AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，HAO1 Antibody (CSB-PA891938LA01HU)，的标记方式是Non-conjugated。对于HAO1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA891938LB01HU HAO1 Antibody, HRP conjugated ELISA
    FITC CSB-PA891938LC01HU HAO1 Antibody, FITC conjugated
    Biotin CSB-PA891938LD01HU HAO1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

问答及客户评论

■ 常见问题解答
Q:

关于HAO-1抗体。我注意到大多数人说免疫原是重组人羟基酸氧化酶1蛋白(161-273AA)，使用这部分蛋白有原因吗？

A:
我们利用该片段制备抗原以制备兔多克隆抗体，因为根据我们对该蛋白的分析，HAO1的161-273氨基酸具有最佳的免疫原性。

靶点详情

  • 功能：
    Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
  • 基因功能参考文献：
    1. The study presents the first spectroscopic evidence of the formation of an intermediate with absorbance features resembling those of a flavosemiquinone in the oxidative half-reaction of glycolate oxidase. PMID: 21141873
    2. The inhibitor heteroatoms interact with five active-site residues that have been implicated in catalysis in homologous flavodehydrogenases of L-2-hydroxy acids. In addition, the chlorophenyl substituent is surrounded by nonconserved hydrophobic residues. PMID: 20054120
    3. These inhibitions suggest that glycolate binds to the active site of the reduced enzyme, and that DCIP also has affinity for the oxidized enzyme. PMID: 17669354
    4. Active site and loop 4 movements within human glycolate oxidase. PMID: 18215067
  • 亚细胞定位：
    Peroxisome.
  • 蛋白家族：
    FMN-dependent alpha-hydroxy acid dehydrogenase family
  • 组织特异性：
    Liver.
  • 数据库链接：

    HGNC: 4809

    OMIM: 605023

    KEGG: hsa:54363

    STRING: 9606.ENSP00000368066

    UniGene: Hs.193640