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Human Hydroxyacid oxidase 1(HAO1) ELISA kit

  • 中文名称：
    人羟基酸氧化酶1(HAO1)酶联免疫试剂盒
  • 货号：
    CSB-EL010127HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human HAO1 ELISA Kit was designed for the quantitative measurement of Human HAO1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.69 pg/mL.

  • 别名：
    (S) 2 hydroxy acid oxidase ELISA Kit; Glycolate oxidase ELISA Kit; GOX ELISA Kit; GOX1 ELISA Kit; HAO1 ELISA Kit; HAOX1 ELISA Kit; HAOX1_HUMAN ELISA Kit; Hydroxyacid oxidase 1 ELISA Kit; MGC142225 ELISA Kit; MGC142227 ELISA Kit; OTTHUMP00000030231 ELISA Kit
  • 缩写：
    HAO1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    18.75 pg/mL-1200 pg/mL
  • 灵敏度：
    4.69 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HAO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:5 Average % 98    
    Range % 93-112    
    1:10 Average % 107    
    Range % 103-109    
    1:20 Average % 93    
    Range % 90-97    
    1:40 Average % 88    
    Range % 86-94    
  • 回收率：
    The recovery of human HAO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 103 99-106    
    EDTA plasma (n=4) 92 90-95    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1200 2.193 2.369 2.281 2.097    
    600 1.700 1.710 1.705 1.521    
    300 1.253 1.262 1.258 1.074    
    150 0.878 0.898 0.888 0.704    
    75 0.532 0.566 0.549 0.365    
    37.5 0.411 0.422 0.417 0.233    
    18.75 0.231 0.222 0.227 0.043    
    0 0.177 0.190 0.184 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
  • 基因功能参考文献：
    1. The study presents the first spectroscopic evidence of the formation of an intermediate with absorbance features resembling those of a flavosemiquinone in the oxidative half-reaction of glycolate oxidase. PMID: 21141873
    2. The inhibitor heteroatoms interact with five active-site residues that have been implicated in catalysis in homologous flavodehydrogenases of L-2-hydroxy acids. In addition, the chlorophenyl substituent is surrounded by nonconserved hydrophobic residues. PMID: 20054120
    3. These inhibitions suggest that glycolate binds to the active site of the reduced enzyme, and that DCIP also has affinity for the oxidized enzyme. PMID: 17669354
    4. Active site and loop 4 movements within human glycolate oxidase. PMID: 18215067
  • 亚细胞定位：
    Peroxisome.
  • 蛋白家族：
    FMN-dependent alpha-hydroxy acid dehydrogenase family
  • 组织特异性：
    Liver.
  • 数据库链接：

    HGNC: 4809

    OMIM: 605023

    KEGG: hsa:54363

    STRING: 9606.ENSP00000368066

    UniGene: Hs.193640