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抗Payload抗体

抗体药物偶联物（ADCs）是一种新型的抗癌药物，结合了单克隆抗体和小分子毒素，具有高度靶向性和高毒素性。由于ADC 给药后会发生payload 的解结合，导致未结合的payload 的增加和ADC 成分（即DAR 分布）的变化。因此，对偶联的抗体和偶联在抗体上的payload 的体内定量分析有助于评估ADC 药效，并有助于评估payload 在靶点的暴露情况，且确定DAR 分布和平均DAR 值的变化对于提供ADC 的稳定性等整体评估至关重要。

j9九游会登录入口首页CUSABIO推出适用于临床前及临床阶段抗体药有效研究工具——抗Payload抗体。可用于ADC药物的血浆/血清动力学分析、药物结合亲和力的测定、DAR值分析以及ADC药物的疗效评估。

抗Payload抗体类型

ADC 各成分与其对应分析方法

ADC 的生物分析需要一种综合的生物分析方法，包括配体结合测定和高效液相色谱串联质谱联用技术（LC-MS/MS），分别用于大分子和小分子生物分析。抗payload 抗体在ADC 的免疫原性分析中至关重要，用于免疫原性分析的桥接检测方法（Bridging LBA）。

Analyte Type Description Typical analytical platforms
Total antibody Total antibody (conjugated or unconjugated) LBA, hybrid LC-MS/MS
Total ADC Total conjugated antibody (DAR > 1)OR LBA
Total payload conjugated to antibody (DAR > 1) LBA hybrid LC-MS/MS
Unconjugated payload Payload not conjugated to antibody LC-MS/MS
Anti-drug antibodies (ADA) Antibodies directed against antibody components of ADC, linker, or drug (binding/neutralizing) LBA

ADC有效荷载-Anti DXD

DXD-新一代ADC最具前景的有效荷载

图1. DXD-新一代ADC最具前景的有效荷载 [1]

应用场景

药代动力学（PK）分析

偶联DAR值定性分析

免疫原性分析

临床前及临床阶段抗药物抗体开发

产品优势

高纯度

纯度经SDS-PAGE验证大于90%

高特异性

能够特异性识别小分子毒素

高灵敏度，高亲和性

经ADC药物结合验证表现出显著活性

易于检测，节省成本

经过HRP标记的抗DXD抗体方便检测，且无需再与二抗结合

专业技术团队

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产品列表

产品货号 产品名称 应用类型
CSB-MA996977I1m DXD Monoclonal Antibody ELISA
CSB-MA996977I1m-B DXD Monoclonal Antibody, HRP conjugated ELISA
CSB-MA996977I2m DXD Monoclonal Antibody ELISA
CSB-MA996977I2m-B DXD Monoclonal Antibody, HRP conjugated ELISA
CSB-MA903890I1m MMAE&MMAF Monoclonal Antibody ELISA
CSB-MA943989I1m Eribulin Monoclonal Antibody ELISA

验证数据

High purity verified by SEC-MALS and SDS-PAGE
(Code: CSB-MA996977I2m)
SDS-PAGE验证

The Mouse Anti-DXD Antibody (Code: CSB-MA996977I2m) has a purity of more than 90% as determined by SEC-MALS and SDS-PAGE.

High purity verified by SEC-MALS and SDS-PAGE
(Code: CSB-MA943989I1m)
SDS-PAGE验证

The Mouse Anti-DXD Antibody (Code: CSB-MA943989I1m) has a purity of more than 98% as determined by SEC-MALS and SDS-PAGE.

The Binding Activity of Disitamab vedotin (Synonyms: RC48) with Anti-MMAE antibody
(Code: CSB-MA903890I1m)
活性验证

Activity: Measured by its binding ability in a functional ELISA. Immobilized Disitamab vedotin (Synonyms: RC48) at 2 μg/mL can bind Anti-MMAE antibody, the EC50 is 11.65 to 15.97 ng/mL.

The Binding Activity of T-DXd(DS-8201) with Anti-DXD antibody
(Code: CSB-MA996977I2m)
活性验证

Activity: Measured by its binding ability in a functional ELISA. Immobilized T-DXd(DS-8201) at 2 μg/mL can bind Anti-DXD antibody, the activity was 4-5 times that of other companies.

定制服务

j9九游会登录入口首页生物已研发多种高纯度、高灵敏度，高亲和力的抗Payload抗体，可用于ADC药物的临床前及临床阶段DAR值和PK分析，可提供定制化的抗Payload抗体，以满足客户的个性化需求。

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参考文献：

[1] Kumar, Seema, ed. An Introduction to Bioanalysis of Biopharmaceuticals. Vol. 57. Springer Nature, 2022.