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XDH Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA785778A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate
      All lanes: Xanthine Oxidase antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 147 kDa
      Observed band size: 147 kDa
    • IHC image of CSB-RA785778A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    XDH encodes xanthine oxidase, an enzyme that catalyzes the oxidation of hypoxanthine to xanthine, and of xanthine to uric acid, the process that reduces molecular oxygen and produces hydrogen peroxide. Xanthine oxidase plays a central role in purine catabolism by utilizing biologically active nucleotides arising from the degradation of nucleic acids and nucleotide mediators. Excessive production and/or inadequate excretion of uric acid results in hyperuricemia. An excess of hydrogen peroxide is toxic to individual cells. The deficiency of xanthine oxidase leads to a condition called hereditary xanthinuria type 1, attributed to a mutation in the XDH gene that leads to decreased amounts of xanthine oxidase production.

    This recombinant XDH antibody was developed with the Single B cell platform. The main process included identification and isolation of single B cells; amplification and cloning of XDH antibody gene; expression, screening, and identification of antibody specificity.  And this XDH antibody has been validated in ELISA, WB, IHC.

  • Uniprot No.：
    P47989
  • 基因名：
    XDH
  • 别名：
    Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase (XD) (EC 1.17.1.4), Xanthine oxidase (XO) (EC 1.17.3.2) (Xanthine oxidoreductase) (XOR)], XDH, XDHA
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Xanthine Oxidase
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    8A9
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).
  • 基因功能参考文献：
    1. The information represented in the study confirmed previous reports. And it may assist to discover and design new backbones as potential XO inhibitors based on natural products. PMID: 29843539
    2. The role of xanthine oxidoreductase and uric acid in metabolic syndrome. PMID: 29733945
    3. Data found that gastric cancer patients adhering to the SNP rs207454 CC genotype had favorable clinical outcome compared with those carrying the AA/AC genotypes, suggesting that the CC allele was a protective allele in the prognosis of gastric cancer by effecting XDH mRNA expression level. PMID: 29551504
    4. this study shows that XOR is required for genotoxic stress-induced NKG2D ligand expression and gemcitabine-mediated antitumor activity PMID: 27494876
    5. Molecular docking simulations clarified that purpurogallins have stronger affinities for XO than corresponding pyrogallols. These results revealed that the potent XO inhibitory activity seemingly observed in pyrogallol is actually derived from its chemical conversion, under alkaline conditions, into purpurogallin. PMID: 28223196
    6. data suggest that Alcohol use disorders augment pulmonary and systemic XOR activity that may contribute to ROS and uric acid generation, promoting inflammation. PMID: 28839105
    7. XO is a useful marker to assess oxidative stress in polycystic ovary syndrome patients PMID: 27295433
    8. xanthine dehydrogensase gene expression and uric acid production is altered in asthma and in human airway epithelial cells PMID: 28863172
    9. Among cardiac patients, left ventricular hypertrophy, low LVEF, and increased BNP were significantly associated with plasma XOR activity independent of various confounding factors. PMID: 28797123
    10. we demonstrated that BP rise over time and/or the risk of hypertension are associated with three SNP in the XOR gene: rs2043013, rs11904439, and rs148756340. PMID: 27607461
    11. Hypoxanthine-xanthine oxidase axis is not involved in the initial phase of clinical transplantation-related ischemia-reperfusion injury in kidney allograft recipients. PMID: 28031169
    12. Serum XOD activity was higher in type 2 DM patients than in control subjects and was higher in diabetic patients with diabetic peripheral neuropathy than in those without neuropathy. PMID: 27965983
    13. XOR activity is correlated with insulin resistance, BMI, and subclinical inflammation. PMID: 28403945
    14. Study showed that DKK3 promotes cell survival during oxidative stress by suppressing XDH expression, thereby abrogating excess reactive oxygen species accumulation and subsequent apoptosis. PMID: 28299863
    15. Results show XOR strictly modulated at the transcriptional and post-translational levels, and its expression and activity are highly variable in cancer and negatively associated with high malignity grade and a worse prognosis. [review] PMID: 26687331
    16. XDH and SUCLA2 genes associated with thiopurine-induced leukopenia can act in a complex interactive manner in patients with Crohn's disease. PMID: 26863601
    17. beneficial effects observed with XO inhibitors on cardiovascular disease in chronic kidney disease may also be due to the reduction of oxidative stress PMID: 26426087
    18. Thyroid stimulating hormone levels are increase in gout patients treated with inhibitors of xanthine oxidoreductase. PMID: 26342297
    19. The XDH gene polymorphisms rs1042039, rs1054889, and rs2073316 were shown to be associated with hypertension in a rural Chinese population. PMID: 26239312
    20. genetic association studies in population in Mexico: Data suggest that SNPs in XO (1936A>G, 2107A>G) are not associated with development of typical adverse reactions to methotrexate in pediatric patients with ALL (acute lymphoblastic leukemia). PMID: 26353179
    21. the essential role of autophagy-regulated early ROS in triggering late apoptotic signaling PMID: 26029922
    22. XOR regulated macrophage IL-1b secretion upon NLRP3 inflammasome activation via xanthine oxidase derived reactive oxygen species. PMID: 25800347
    23. In this review, we focus on studies of XOR inhibitors and their implications for understanding the chemical nature and reaction mechanism of the Moco active site of XOR PMID: 25501928
    24. xanthine oxidase-mediated ROS production is required for PTN-induced cell migration through the cell membrane functional complex of alphanubeta3 and RPTPbeta/zeta and activation of c-src, PI3K and ERK1/2 kinases PMID: 25582077
    25. Data suggest that, with aging, up-regulation of reactive oxygen species derived from XO down-regulates uric acid excretion via down-regulation of active homodimer breast cancer resistance protein (BCRP1, uric acid efflux transporter) in ileal mucosa. PMID: 26119820
    26. Xanthine oxidase is activated in THP-1 human myeloid cells in response to pro-inflammatory and growth factor stimulation. PMID: 25200751
    27. Xanthine oxidoreductase has roles in atherosclerosis pathogenesis [review] PMID: 25463089
    28. Xanthine dehydrogenase takes part in retinoic acid biosynthesis in the thyroid glandular epithelia. PMID: 24506204
    29. The polymorphisms rs1052133 on OGG1 and rs207454 on XDH show a trend of association with overall survival. PMID: 23443115
    30. Results confirm the role of XO enzyme in ischemic heart disease. PMID: 24092361
    31. These results supported the possibility that XO/XD and AO can contribute to nitric oxide generation. PMID: 25537183
    32. XO is present in the pathogenesis of metabolic syndrome. PMID: 24967004
    33. In infection with E. coli both XO and MPO urine levels were increased. PMID: 24591758
    34. these data support a novel function of the XO-NFAT5 axis in macrophage activation and TLR-induced arthritis PMID: 25044064
    35. XOR activity is significantly associated with NAFLD PMID: 25185415
    36. This study reveals a noncanonical molecular pathway in which, after the inhibition of cathepsin S, autophagy induces early reactive oxygen species production for oxidative DNA damage and cell death through xanthine oxidase. PMID: 23892358
    37. An upregulated XOD may be implicated in hrmodialysis-induced oxidative injury. PMID: 23819009
    38. xanthine oxidoreductase may have a function in the formation or persistence of chronic wounds PMID: 24357442
    39. Inhibition of XO increased survival of human embryo brain-derived cells in culture. . There was a a reversed correlation between the activities of XO and DPD over 12 days under normal conditions as well as in the presence of XO and DPD inhibitors. PMID: 23597150
    40. Case Report: hereditary xanthinuria with xanthine dehydrogenase deficiency due to novel sequence variations presenting as rheumatoid arthritis. PMID: 20077140
    41. Xanthine dehydrogenase/oxidase is a moonlighting protein that has two distinct catalytic activities as a dehydrogenase and an oxidase. PMID: 17301077
    42. SNPs at the 5'-nucleotidase and xanthine oxidase genes influence the risk of noncirrhotic portal hypertension in HIV patients treated with didanosine. PMID: 23315321
    43. Data suggest that XOD is up-regulated in lens of patients with (1) diabetes type 2 with cataracts and (2) senile cataracts; blood HbA1C levels (or poor glycemic control) are positively correlated with lens XOD activity as well as serum XOD activity. PMID: 23142692
    44. Site-directed mutagenesis followed by expression analysis in Escherichia coli revealed that not only the frame shift mutation 141insG impairs XDH activity, but also the missense mutation C2729T. PMID: 22981351
    45. Xanthine oxidase released during enteropathogenic Escherichia coli and Shiga-toxigenic Escherichia coli infection may serve as a virulence-inducing signal to the pathogen and not solely as a protective host defense. PMID: 23340314
    46. Xanthinuria Type I is caused by mutations in the xanthine dehydrogenase gene (XDH). We report on a patient suffering from xanthinuria. Genomic DNA was screened for point mutations and imbalances in the XDH gene by sequencing and microarray typing. PMID: 23249873
    47. XDH genetic polymorphisms may influence azathioprine treatment outcome in patients with kidney transplants. PMID: 22495427
    48. There was no significant difference in XO activity between Swedes and Koreans PMID: 22105431
    49. high tumoral XDH expression is an independent predictor of a poor prognosis in patients with adenocarcinoma of the lung. PMID: 22678977
    50. The results indicated that anti-HXOR antibodies in synovial fluids have a protective role as high concentrations against XOR were detected in inflammatory arthritis. PMID: 21644044

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  • 相关疾病：
    Xanthinuria 1 (XAN1)
  • 亚细胞定位：
    Cytoplasm. Peroxisome. Secreted.
  • 蛋白家族：
    Xanthine dehydrogenase family
  • 组织特异性：
    Detected in milk (at protein level).
  • 数据库链接：

    HGNC: 12805

    OMIM: 278300

    KEGG: hsa:7498

    STRING: 9606.ENSP00000368727

    UniGene: Hs.250