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  4. Recombinant Human Somatotropin (GH1) (Active)

Recombinant Human Somatotropin (GH1) (Active)

In Stock
  • 货号：
    CSB-MP009407HU
  • 规格：
    ￥1368
  • 图片：

    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/ml can bind human PRLR(CSB-MP018727HU1), the EC50 of the protein is 60.71-69.65 ng/ml.

      Biological Activity Assay

    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/ml can bind human GHR(CSB-MP009411HU), the EC50 of the protein is 19.28-25.29 ng/ml.

      Biological Activity Assay

    • Human GH1 protein his/myc tag (CSB-MP009407HU) captured on COOH chip can bind Human GHR protein Fc tag (CSB-MP009411HU) with an affinity constant of 6.1 nM as detected by LSPR Assay.

      Biological Activity Assay
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized human GH1 at 2 μg/ml can bind Biotinylated human GHR (CSB-MP009411HUj1-B), the EC50 is 2.067-3.208 ng/ml. Biological Activity Assay
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 内毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    ①Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/ml can bind human PRLR(CSB-MP018727HU1), the EC50 of the protein is 60.71-69.65 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/ml can bind human GHR(CSB-MP009411HU), the EC50 of the protein is 19.28-25.29 ng/ml.③Human GH1 protein his/myc tag (CSB-MP009407HU) captured on COOH chip can bind Human GHR protein Fc tag (CSB-MP009411HU) with an affinity constant of 6.1 nM as detected by LSPR Assay.④Measured by its binding ability in a functional ELISA. Immobilized human GH1 at 2 μg/ml can bind Biotinylated human GHR (CSB-MP009411HUj1-B), the EC50 is 2.067-3.208 ng/ml.
  • 基因名：
  • Uniprot No.：
  • 别名：
    gH; GH-N; GH1; GHB5; GHN; Growth hormone 1; Growth hormone; Growth hormone B5; Growth hormone; normal; Growth hormone; pituitary; HG1; hGH-N; IGHD1B; Pituitary growth hormone; RNGHGP; SOMA_HUMAN; Somatotropin
  • 分子结构：
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Mammalian cell
  • 分子量：
    27.2 kDa
  • 表达区域：
    27-217aa
  • 氨基酸序列
    FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The Recombinant Human Somatotropin GH1 is a recombinant hormone formed in mammalian cells. A DNA sequence encoding the growth hormone 1 (Phe21-Phe217) was fused with a polyhistidine tag at N-terminal and a Myc-tag at C-terminal. This sequence codes for the protein with 191 amino acids, and has a calculated molecular mass of 27.2 kDa (191 amino acids).
    Human Somatotropin/Growth Hormone belongs to the growth factor family of hormones. It is secreted and stored in the anterior pituitary gland. It stimulates growth by aiding the uptake of amino acids, facilitating protein synthesis, and stimulating cellular proliferation. Most of its effects are accomplished through IGF-1.
    Deletions and mutations in the gene coding for growth hormone lead to growth hormone deficiency. Growth hormone deficiency, in turn, plays a part in a couple of diseases.
    The Recombinant Human Somatotropin GH1 finds application in studies involving growth hormone deficiency, Kowarski disease, Turner syndrome, cancer, metabolic disorders, and congestive heart failure.
    It has an endotoxin content lower than 1 EU/µg and purity over 90%, as verified by LAL and SDS-PAGE, respectively.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

平均分:
5.0分 - 1 个评价

实验应用：结合实验/蛋白间互作

产品评价： 收到产品后，我测试GH1与GHR的结合活性，GH1在浓度1 μg/ml 时可以结合人GHR， EC50为24.96-33.39 ng/ml，蛋白有完整的生物活性。进行亲和力测试，显示结果较好！蛋白可重复性好，性能稳定，值得购买！

By 王老师

靶点详情

  • 功能：
    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 基因功能参考文献：
    1. Significant correlations were observed between GH concentration and impairments on several EDI-2 subscales (drive for thinness, body dissatisfaction, interoceptive awareness, sense of ineffectiveness, interpersonal distrust, maturity fear) and on SCL-90 subitems (depression, hostility, obsessivity compulsivity, anxiety), suggesting a possible hormonal modulatory effect on specific aspects of eating disorders. PMID: 29179911
    2. Patients examined at 1 year or several years from complicated mild traumatic brain injury had a similarly high occurrence of isolated GH deficiency PMID: 27878771
    3. Single nucleotide variant in GH1 gene is associated with isolated growth hormone deficiency. PMID: 28910730
    4. In newborns, serum PRL and hGH levels show a positive correlation that can be explained by common regulatory factors or a drift phenomenon. A higher gestational week is associated with a higher PRL/hGH ratio. PMID: 28700563
    5. This review describes the endocrine profile of centenarians concerning the GH/IGF-I/insulin system, focusing on the relevance of this pathway on the modulation of ageing and longevity. PMID: 27932301
    6. Data suggest that patients with NICTH (non-islet cell tumor hypoglycemia) exhibit low serum growth hormone levels during hypoglycemic episodes. [Retrospective Study & REVIEW of Case Reports] PMID: 28529277
    7. Homozygous GH1 deletion is associated with growth hormone deficiency. PMID: 28525353
    8. Human Growth Hormone Inhibits CLAUDIN-1 Expression Through Activation of Signal Transducer and Activator of Transcription 3 (STAT3). PMID: 28617312
    9. Serum GH was unrelated to type 2 diabetes, fasting blood glucose, or HbA1c level. PMID: 27060213
    10. GH1 and GHRHR screening revealed eleven variations in 24 (21%) patients with isolated growth hormone deficiency of which, four were novel deleterious, one novel non-pathogenic and six reported changes. PMID: 27114065
    11. The results suggest that GH regulates energy metabolism directly in myocytes and that UCP2 participates in the signal transduction pathway that functions downstream of the GHR/JAK/STAT. PMID: 27150070
    12. These results implicate TIMP3 as a modulator of cell surface GHR abundance and the ability of GH to promote cellular signaling. PMID: 27075707
    13. Children with GH excess underwent medical treatment with lanreotide and a minimum clinical/biochemical follow up of 2 years is reported. The present study demonstrates that GH excess should be considered as a relative frequent endocrine manifestation in NF1 patients, similarly to central precocious puberty PMID: 28631895
    14. Thus, GHRH analogs of the Miami series powerfully suppress tumor growth, but have only a weak endocrine GH inhibitory activity. The suppression of tumor growth could be induced in part by the downregulation of GHRH receptors levels. PMID: 28130121
    15. the levels and kinetics of phosphorylation mediated by the main signalling proteins triggered by 22K-GH or 20K-GH were not exactly the same. PMID: 28427901
    16. A negative regulation of locally produced GH by androgens/AR in Prostate cancer cells following treatment with AR agonists (R1881) and antagonists (enzalutamide, bicalutamide). PMID: 28444169
    17. The intrinsic amyloidogenicity of growth hormone, in the presence of contaminating prion protein (and perhaps prolactin as well) and amyloid-beta contained in some cadavers' pituitaries, may have led to the observed co-occurring of Creutzfeldt-Jakob disease and Alzheimer's disease. PMID: 27214308
    18. GH potentially negatively modulates the maturation and accumulation of lipid in adipocytes. PMID: 27802441
    19. To our knowledge, c.-223C>T is the first homozygous point mutation in the GH1 promoter that leads to short stature due to idiopathic growth hormone deficiency. PMID: 27252485
    20. Data show that the recombinant protein produced by the plasmid-free E coli strain was purified and characterized to be human growth hormone (hGH). PMID: 27542624
    21. Our results suggest that the known protective effect of GH signaling deficiency on neoplastic tissue growth is mediated, at least partially, by regulating p53 expression PMID: 27226307
    22. evidence that hGH synthesis follows a diurnal rhythm and of dynamic associations of the circadian machinery with a component of a chromosomal structure of the hGH1 locus that is essential for efficient expression. PMID: 27151213
    23. Gene polymorphism of leptin (loci rs7799039) and leptin receptor (loci rs1137101) are correlated with Growth hormone deficiency susceptibility. PMID: 26915772
    24. These results showed that hybrid training system on a cycle ergometer (CE) was more efficient in stimulating acute increases in GH, lactate and IL-6 than CE at the same workload. PMID: 26522057
    25. Growth Hormone 1 T1663A Polymorphism were at a decreased risk of breast cancer. PMID: 26225688
    26. This is the first report of a family suffering from short stature caused by autosomal dominant form of GH deficiency II, which severely affects intracellular GH folding and stability as well as secretion PMID: 26485222
    27. GH and IGF-1 suppression is maintained for up to 25 months during pasireotide LAR treatment of acromegaly. PMID: 25103549
    28. In women with normal somatotroph function, GH levels do not change in the first trimester of pregnancy. PMID: 25179796
    29. These data on pregnancy outcomes in a large group of women with hypopituitarism revealed no relationship between GH replacement therapy regimens and pregnancy outcomes. PMID: 26256649
    30. Effect of oral glucose administration on rebound growth hormone release in normal and obese women: the role of adiposity, insulin sensitivity and ghrelin. PMID: 25782001
    31. the phenotype of MIP-FoxM1-hGH mice is due primarily to hGH activity and that the FoxM1 protein remains largely inactive PMID: 26202070
    32. Case Report: of Klinefelter sydnrome with short stature due to growth hormone deficiency. PMID: 25241616
    33. Suggest growth hormone deficiency may be common feature in vernal keratoconjuntivitis patients. PMID: 25079463
    34. Human Growth Hormone stimulates the microRNA 96-182-183 cluster, which promotes the epithelial-mesenchymal transition and invasion in breast cancer PMID: 25873390
    35. The results demonstrate that activation of noncoding transcription reflects an autonomous activity of the human growth hormone long-range enhancer that is fully independent of interactions with linked gene promoters and occurring in spatial and temporal synchrony with initiation of GH expression in the embryonic pituitary. PMID: 25662214
    36. Analysis of GH1 in a cohort of Brazilian patients revealed that the autosomal recessive form of isolated growth hormone deficiency(IGHD) was more common than the dominant one, and both were found only in severe IGHD. PMID: 25116472
    37. Recipients showed a rapid recovery of the GH/IGF1 hormonal axis and liver function after LDLT, whereas donors showed altered GH signaling and regenerative delay in the early days after living donation. PMID: 24889799
    38. JAK2 is activated by growth hormone and other cytokines. (Review) PMID: 25656053
    39. Genotyping contributed to the diagnosis of children with suspected growth hormone insensitivity and short stature. PMID: 25411237
    40. hGH production is extremely sensitive to increased caloric intake. PMID: 25295535
    41. After LT, GH levels correlate with the extent of cytolysis, while IGF-1 is an indicator of liver synthetic function recovery. IGF-1 levels >90 mug/L (day 15-30) seem to be an indicator of short-term survival. PMID: 24804205
    42. In this review we highlight the evidence of extrapituitary synthesis of GH in humans. [review] PMID: 24642386
    43. This review summarizes findings of growth hormone's influence on in utero and neonatal cellular and metabolic profiles related to bone and adipose tissue. PMID: 25015810
    44. The study presents experimental data for the mechanism of thiol-disulfide exchange in tryptic peptides derived from human growth hormone in aqueous solution. PMID: 24549831
    45. we present the results of screening for mutations in GH1 and GHRHR genes in a large cohort of Argentinian patients with IGHD. These suggest that the p.Arg183His mutation associated with the type II dominant form of IGHD might be relatively common. PMID: 23789946
    46. In the first family a novel splice site mutation in GH1 was identified (c.172-1G>C, IVS2-1G>C). In two other families a previously reported splice site mutation (c.291+1G>A, IVS3+1G>A) was found. PMID: 24280736
    47. Bilateral involvement of a pituitary adenoma and severely decreased immediate postoperative serum GH levels at 72 hours after transsphenoidal adenomectomy may be independent risk factors for accelerated GH deficiency in acromegalic patients. PMID: 24972779
    48. The activity of autocrine GH may be distinct from that of endocrine GH in prostate cancer cells. PMID: 23238889
    49. robust GH-stimulated hepatic Igf1 gene transcription utilizes tissue-specific mechanisms of epigenetic regulation that are established independent of GH signaling. PMID: 24109593
    50. meta-analysis indicates that GH1 T1663A polymorphism may contribute to the risk of colorectal cancer, especially among Asian populations PMID: 24464925

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  • 相关疾病：
    Growth hormone deficiency, isolated, 1A (IGHD1A); Growth hormone deficiency, isolated, 1B (IGHD1B); Kowarski syndrome (KWKS); Growth hormone deficiency, isolated, 2 (IGHD2)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 数据库链接：

    HGNC: 4261

    OMIM: 139250

    KEGG: hsa:2688

    STRING: 9606.ENSP00000312673

    UniGene: Hs.655229