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  4. Recombinant Human Atypical chemokine receptor 1 (ACKR1) (Active)

Recombinant Human Atypical chemokine receptor 1 (ACKR1) (Active)

In Stock
  • 货号：
    CSB-CF624105HU
  • 规格：
    ￥9720
  • 图片：
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized ACKR1 at 1 μg/ml can bind human CCL2, the EC50 of human CCL2 protein is 48.64-60.24 μg/ml.

    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性：
    Measured by its binding ability in a functional ELISA. Immobilized ACKR1 at 1 μg/ml can bind human CCL2, the EC50 of human CCL2 protein is 48.64-60.24 μg/ml.
  • 基因名：
  • Uniprot No.：
  • 别名：
    ACKR1; DARC; FY; GPD; Atypical chemokine receptor 1; Duffy antigen/chemokine receptor; Fy glycoprotein; GpFy; Glycoprotein D; Plasmodium vivax receptor; CD antigen CD234
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    41.1 kDa
  • 表达区域：
    1-336aa
  • 氨基酸序列
    MGNCLHRAELSPSTENSSQLDFEDVWNSSYGVNDSFPDGDYGANLEAAAPCHSCNLLDDSALPFFILTSVLGILASSTVLFMLFRPLFRWQLCPGWPVLAQLAVGSALFSIVVPVLAPGLGSTRSSALCSLGYCVWYGSAFAQALLLGCHASLGHRLGAGQVPGLTLGLTVGIWGVAALLTLPVTLASGASGGLCTLIYSTELKALQATHTVACLAIFVLLPLGLFGAKGLKKALGMGPGPWMNILWAWFIFWWPHGVVLGLDFLVRSKLLLLSTCLAQQALDLLLNLAEALAILHCVATPLLLALFCHQATRTLLPSLPLPEGWSSHLDTLGSKS
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Has a promiscuous chemokine-binding profile, interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. Acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1, TARC and also for the malaria parasites P.vivax and P.knowlesi. May regulate chemokine bioavailability and, consequently, leukocyte recruitment through two distinct mechanisms: when expressed in endothelial cells, it sustains the abluminal to luminal transcytosis of tissue-derived chemokines and their subsequent presentation to circulating leukocytes; when expressed in erythrocytes, serves as blood reservoir of cognate chemokines but also as a chemokine sink, buffering potential surges in plasma chemokine levels.
  • 基因功能参考文献：
    1. The mean number of rosettes formed by erythrocytes surrounding transfected mammalian COS-7 cells which expressed Plasmodium knowlesi Duffy binding protein differ between erythrocytes' Duffy antigen phenotypes. Plasmodium knowlesi Duffy binding protein displays higher binding to Fy(a+/b+) erythrocytes than to Fy(a+/b-) erythrocytes. PMID: 30257710
    2. The atypical chemokine receptor 1 polymorphism (rs12075) does not affect susceptibility to hepatitis C virus. PMID: 28443566
    3. The risk of Plasmodium vivax malaria associated with genetic variation of DARC in Thai patients is reported. PMID: 29620340
    4. Our findings in mice suggest that DARC alters the severity and resolution of AHR. These findings are complimented by our human analyses in which select DARC SNPs are associated with worse asthma control and symptoms. PMID: 28471517
    5. DARC expression in cancer cells inhibits pancreatic ductal adenocarcinoma progression by suppressing STAT3 activation through the inhibition of CXCR2 signaling PMID: 28214673
    6. This study reports 1-3 occurrences of P. vivax infection in each of 25 Duffy-negative children at six time points over two rainy seasons and the beginning of the third season. PMID: 28749772
    7. Data suggest that Type II congenital smell loss patients who exhibit both type I hyposmia and hypogeusia are genetically distinct from all other patients with Type II congenital smell loss. This distinction is based on decreased Fy(b) expression which correlated with abnormalities in two sensory modalities (hyposmia type I and hypogeusia). PMID: 27968956
    8. The data suggest that selective exposure of the Duffy binding protein (DBP) binding site within DARC is key to the preferential binding of DBP to immature reticulocytes, which is the potential mechanism underlying the preferential infection of a reticulocyte subset by P vivax. PMID: 28754683
    9. ACKR1 regulates neutrophil counts in blood. Lack of ACKR1 on nucleated erythroid cells together with its expression in endothelial cells causes neutropenia. PMID: 28553950
    10. population genetic analysis of DARC shows its association with malaria resistance PMID: 28282382
    11. Molecular identification of rare FY*Null and FY*X alleles in Caucasian thalassemic family from Sardinia. PMID: 25921504
    12. We showed that DARC expression is down-regulated in CRC and associated with clinical pathological features and MVD of CRC. DARC might be involved in tumorigenesis, progression, angiogenesis, and metastasis of CRC. PMID: 26096170
    13. DARC is required for Staphylococcus aureus-mediated lysis of human erythrocytes, and DARC overexpression is sufficient to render cells susceptible to toxin-mediated lysis. PMID: 26320997
    14. the odds for neutropenia in the ACKR1-null (FY-) individuals were 46-fold higher than for FY+ individuals (Crude odds ratio = 46, 95% confidence interval = 8.03-263, P < 0.001) PMID: 25817587
    15. study demonstrates the association of SNP rs12075 from DARC gene with the levels of serum IL8 PMID: 25647274
    16. DARC levels are elevated in human keloid fibroblasts and might inhibit the secretion of CCL2. PMID: 26045366
    17. Through molecular genotyping we also identified polymorphisms in RhCE, Kell, Duffy, Colton, Lutheran and Scianna loci in donors and patients. PMID: 25582271
    18. The majority of our population is heterozygous for Duffy antigens a and b. PMID: 24929836
    19. Duffy blood group PMID: 24845979
    20. Isothermal titration calorimetry studies show these structures are part of a multi-step binding pathway, and individual point mutations of residues contacting DARC result in a complete loss of RBC binding by DBP-RII. PMID: 24415938
    21. DARC polymorphisms may influence the naturally acquired inhibitory anti-Duffy binding protein II immunity PMID: 24710306
    22. DARC facilitates CXCL1 inhibition of airway smooth muscle cell migration via modulation of the ERK-1/2 MAP kinase signaling pathway. PMID: 24981451
    23. The Fy(a-b-) phenotype in three siblings of the Polish family was caused by the FY(*)X/FY(*)B-33 genotype. PMID: 23820435
    24. DARC antigen is associated with a likelihood for patients to develop leg ulcers in sickle cell disease. PMID: 23753024
    25. Effect of genetic variants in two chemokine decoy receptor genes, DARC and CCBP2, on metastatic potential of breast cancer. PMID: 24260134
    26. co-expression of DARC, D6, and CCX-CKR significantly associated with higher survival in gastric cancer PMID: 23462454
    27. The in fl uence of DARC gene expression on bone mineral density of the mandible was not con fi rmed. PMID: 22910367
    28. DARC and BCAM expression was associated with pathogenesis of thyroid carcinoma and correlated with clinical-pathological features. PMID: 23168236
    29. The high frequency of the FYES allele that silences erythroid expression of the Duffy antigen offers a biologically plausible explanation for the lack of Plasmodium vivax infections observed. PMID: 23347639
    30. Although lower, the risk of Duffy-negatives to experience a P. vivax blood stage infection was not significantly different to that of Duffy-positives. PMID: 23259672
    31. strong associations between SNPs in DARC gene, particularly rs12075, and serum MCP-1 levels identify a potential candidate gene that can be explored for its role in inflammatory pathways; replication of European GWAS of MCP-1 in study of Hispanic children confirms importance of DARC in regulation of MCP-1 PMID: 23017229
    32. vivax malaria incidences over the past five years in an Indian population were significantly negatively and positively associated with the frequencies of the FY*A and FY*B alleles of the Duffy gene, respectively. PMID: 23028857
    33. platelet molecule platelet factor 4 (PF4 or CXCL4) and the erythrocyte Duffy-antigen receptor (Fy) are necessary for platelet-mediated killing of Plasmodium falciparum parasites. PMID: 23224555
    34. Data suggest that DARC (Duffy antigen receptor for chemokines) gene may play an important role in regulating the metabolisms of both lean body mass (LBM) and age at menarche (AAM). PMID: 22744181
    35. We developed a phylogenetic tree for DARC alleles and postulated a distinct FY*B allele as ancestral for the extant DARC alleles in humans. PMID: 22082243
    36. Our results provide evidence that the functional rs2814778 polymorphism in the gene encoding DARC is associated with worse clinical outcomes among African Americans with ALI, possibly via an increase in circulating IL-8. PMID: 22207676
    37. Data show that in ancestry to the African continent (AA) subjects, the region surrounding the Duffy antigen/chemokine receptor gene (DARC) on 1q21 exhibited significant association with white blood cell count (WBC) levels. PMID: 22037903
    38. findings show that Fy(a), compared with Fy(b), significantly diminishes binding of Pv Duffy binding protein (PvDBP) at the erythrocyte surface, and is associated with a reduced risk of clinical Plasmodium vivax in humans PMID: 22123959
    39. It seems that this version of DARC receptor is a powerful facilitator of chemokine transcytosis and subsequently leukocyte migration into GVHD target organs. PMID: 21784153
    40. Here we describe the recent success in mapping the gene that underlies benign neutropenia in African American populations. We discuss the known function of the gene and consider potential mechanisms for the effect on neutropenia.[review] PMID: 22097233
    41. despite an association of CCL2 serum levels with the severity of liver fi brosis, a functional SNP in the CCL2 scavenger receptor DARC is not directly associated with the occurrence or the severity of liver disease in hepatitis C infection infection PMID: 21156192
    42. DARC -46C/C results in loss of DARC expression on erthyrocytes (Duffy-null) and resistance to Plasmodium vivax malaria, and subjects with this genotype had pre-HIV seroconversion neutrophil counts of <2500 cells/mm(3). PMID: 21507922
    43. DARC and D6, the most studied members of this group of molecules, are reviewed. PMID: 21151196
    44. results do not support an effect of erythrocyte DARC expression on the risk or progression of prostate cancer in men of African descent. PMID: 20596779
    45. This activation state associated with DARC RBC expression may influence the intensity of the inflammatory responses encountered in sickle cell anemia and participate in its interindividual clinical expression variability. PMID: 21088296
    46. Duffy Antigen Receptor for Chemokines (DARC) is an unusual transmembrane chemokine receptor which binds the two main chemokine families. PMID: 20655787
    47. Plasmodium vivax is able to infect and cause Malaria vivax in Duffy-negative people. PMID: 20655790
    48. Results indicate that one of the primary mechanisms by which P. vivax evades host immunity is through DARC indirectly down-regulating humoral responses against erythrocytic invasion and development. PMID: 20664684
    49. Genome-wide association analysis finds strong association for serum MCP-1 with a nonsynonymous polymorphism, rs12075 (Asp42Gly) in DARC. PMID: 20040767
    50. Duffy-positive sickle cell anemia patients exhibited higher counts of white blood cells, polynuclear neutrophils, higher plasma levels of IL-8 and RANTES than Duffy-negative patients. PMID: 20347396

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  • 亚细胞定位：
    Early endosome. Recycling endosome. Membrane; Multi-pass membrane protein. Note=Predominantly localizes to endocytic vesicles, and upon stimulation by the ligand is internalized via caveolae. Once internalized, the ligand dissociates from the receptor, and is targeted to degradation while the receptor is recycled back to the cell membrane.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Atypical chemokine receptor subfamily
  • 组织特异性：
    Found in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues exp
  • 数据库链接：

    HGNC: 4035

    OMIM: 110700

    KEGG: hsa:2532

    STRING: 9606.ENSP00000357103

    UniGene: Hs.153381