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  4. Rat secretory immunoglobulin A,sIgA ELISA Kit

Rat secretory immunoglobulin A,sIgA ELISA Kit

  • 中文名称：
    大鼠分泌型免疫球蛋白A(sIgA)酶联免疫试剂盒
  • 货号：
    CSB-E08412r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat sIgA ELISA Kit was designed for the quantitative measurement of Rat sIgA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL .

  • 缩写：
    sIgA
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %102
    Range %97-106
    1:200Average %90
    Range %86-96
    1:400Average %97
    Range %92-103
    1:800Average %88
    Range %84-92
  • 回收率：
    The recovery of rat sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8480-88
    EDTA plasma (n=4)9591-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.511 2.371 2.441 2.302
    252.046 1.976 2.011 1.872
    12.51.512 1.501 1.507 1.368
    6.250.979 1.003 0.991 0.852
    3.120.482 0.518 0.500 0.361
    1.560.334 0.359 0.347 0.208
    0.780.241 0.229 0.235 0.096
    00.143 0.135 0.139 　
  • 数据处理：
  • 货期：
    3-5 working days

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