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  4. Pig secretory immunoglobulin A,SIgA ELISA Kit

Pig secretory immunoglobulin A,SIgA ELISA Kit

  • 中文名称：
    猪分泌型免疫球蛋白A(sIgA)酶联免疫试剂盒
  • 货号：
    CSB-E12063p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig sIgA ELISA Kit was designed for the quantitative measurement of Pig sIgA protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.15 μg/mL-37.5 μg/mL and the sensitivity is 0.15 μg/mL.

  • 缩写：
    sIgA
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.15 μg/mL-37.5 μg/mL
  • 灵敏度：
    0.15 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:200 Average % 92  
    Range % 88-97  
    1:400 Average % 94  
    Range % 90-98  
    1:800 Average % 87  
    Range % 85-94  
    1:1600 Average % 96  
    Range % 91-103  
  • 回收率：
    The recovery of pig sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-99  
    EDTA plasma (n=4) 103 98-108  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    37.5 0.120 0.115 0.118    
    9.38 0.456 0.463 0.460    
    2.34 0.901 0.877 0.889    
    0.58 1.432 1.527 1.480    
    0.15 2.103 2.189 2.146    
    0 2.544 2.753 2.649    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型：血清

样品信息：其他 猪

稀释比：没有稀释

产品评价： 标曲准确，样本数值在标曲范围之内，符合预期结果。

By 谢老师

问答及客户评论

■ 常见问题解答
Q:

Could you indicate me if your kit could be valid for feces samples? Any recommendations for this type of samples?

A:
Thanks for your inquiry.
Here advice the customer to arrange preliminary experiment if you want to detect feces sample.
Below is our sample method for your reference:
Feces Dilute samples with appropriate Sample Diluent.
This can be achieved by adding 0.1g sample to appropriate Sample Diluent.
Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately.Pls let me know if you have any further questions. Thank you.