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  4. Human secretory immunoglobulin A,SIgA ELISA Kit

Human secretory immunoglobulin A,SIgA ELISA Kit

  • 中文名称：
    人分泌型免疫球蛋白A(SIgA)酶联免疫试剂盒
  • 货号：
    CSB-E09235h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

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  • 产品描述：

    This Human SIgA ELISA Kit was designed for the quantitative measurement of Human SIgA protein in saliva, urine, tear. It is a Sandwich ELISA kit, its detection range is 0.012 μg/mL-50 μg/mL and the sensitivity is 0.012 μg/mL.

  • 缩写：
    SIgA
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    saliva, urine, tear
  • 检测范围：
    0.012 μg/mL-50 μg/mL
  • 灵敏度：
    0.012 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average Corrected  
    50 2.207 2.320 2.264 2.193  
    12.5 2.104 2.234 2.169 2.098  
    3.125 1.855 1.846 1.851 1.780  
    0.781 1.539 1.551 1.545 1.474  
    0.195 0.775 0.801 0.788 0.717  
    0.049 0.416 0.401 0.409 0.338  
    0.012 0.192 0.196 0.194 0.123  
    0 0.069 0.072 0.071 　  
  • 本试剂盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human SIgA antibody.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial HRP-conjugated SIgA antibody (100 x concentrate) (120 μl/bottle) ---Bind to the CEA in the samples or standards and react with the substrate to make the solution chromogenic.
    • Two vials Sample Diluent (20 ml/bottle)---Dilute the sample.
    • One vial Wash Buffer (25x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB substrate (10ml/bottle) ---Interact with TMB to elicit a chromogenic reaction.
    • One vial Stop Solution (10ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

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