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  4. Mouse secretory immunoglobulin A,sIgA ELISA Kit

Mouse secretory immunoglobulin A,sIgA ELISA Kit

  • 中文名称：
    小鼠分泌型免疫球蛋白A(sIgA)酶联免疫试剂盒
  • 货号：
    CSB-E08413m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse sIgA ELISA Kit was designed for the quantitative measurement of Mouse sIgA protein in serum, plasma, tissue homogenates, bronchoalveolar lavage fluid. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL .

  • 缩写：
    sIgA
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, bronchoalveolar lavage fluid
  • 检测范围：
    15.6 ng/mL-1000 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:20Average %92
    Range %87-95
    1:40Average %101
    Range %97-104
    1:80Average %93
    Range %87-97
    1:160Average %98
    Range %92-102
  • 回收率：
    The recovery of mouse sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-97
    EDTA plasma (n=4)9692-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    10002.486 2.466 2.476 2.312
    5001.772 1.841 1.807 1.643
    2501.232 1.222 1.227 1.063
    1250.760 0.748 0.754 0.590
    62.50.464 0.481 0.473 0.309
    31.20.325 0.312 0.319 0.155
    15.60.239 0.229 0.234 0.070
    00.166 0.162 0.164 　
  • 数据处理：
  • 货期：
    3-5 working days

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