Gpc3

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human GPC3 (G537R) at 5 μg/ml can bind Anti-GPC3 recombinant antibody, the EC₅₀ is 4.739-7.092 ng/ml.

Western Blot
Positive WB detected in: 293 whole cell lysate, K562 whole cell lysate All lanes: GPC3 antibody at 0.9μg/ml. Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 66, 60, 69 KDa Observed band size: 66 KDa

Immunofluorescence staining of HepG2 cells with CSB-RA009705A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).