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Rat Glypican-3(GPC3) ELISA kit

  • 中文名称：
    大鼠磷脂酰肌醇蛋白聚糖3(GPC3)酶联免疫试剂盒
  • 货号：
    CSB-EL009705RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat GPC3 ELISA Kit was designed for the quantitative measurement of Rat GPC3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Gpc3 ELISA Kit; Glypican-3 ELISA Kit; Intestinal protein OCI-5) [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GPC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %85-99
    1:2Average %107
    Range %97-110
    1:4Average %89
    Range %83-93
    1:8Average %99
    Range %91-104
  • 回收率：
    The recovery of rat GPC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9484-99
    EDTA plasma (n=4)10094-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.506 2.415 2.461 2.280
    5002.091 1.974 2.033 1.852
    2501.353 1.332 1.343 1.162
    1250.813 0.798 0.806 0.625
    62.50.484 0.456 0.470 0.289
    31.20.408 0.392 0.400 0.219
    15.60.274 0.267 0.271 0.090
    00.183 0.179 0.181
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cell surface proteoglycan that bears heparan sulfate. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. Inhibits the dipeptidyl peptidase activity of DPP4. Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4. Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis. Required for coronary vascular development. Plays a role in regulating cell movements during gastrulation.
  • 基因功能参考文献：
    1. Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of hepatocellular carcinoma. PMID: 21669573
    2. Gpc3 is a marker of hepatic progenitor cells and of early liver lesions. PMID: 17117158
    3. GPC3 may be a negative regulator of liver regeneration and hepatocyte proliferation, and that this regulation may involve CD81. PMID: 19574424
  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
  • 蛋白家族：
    Glypican family
  • 数据库链接：

    KEGG: rno:25236

    STRING: 10116.ENSRNOP00000043344

    UniGene: Rn.9717