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  4. Recombinant Human Indian hedgehog protein (IHH), partial (Active)

Recombinant Human Indian hedgehog protein (IHH), partial (Active)

In Stock
  • 货号：
    CSB-AP000421HU
  • 规格：
    ￥852
  • 图片：
  • 其他：

产品详情

  • 纯度：
    >96% as determined by SDS-PAGE.
  • 内毒素：
    Less than 1.0 EU/μg as determined by LAL method.
  • 生物活性：
    Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 μg/ml.
  • 基因名：
  • Uniprot No.：
  • 别名：
    BDA1; HHG-2; HHG2; IHH; IHH_HUMAN; Indian Hedgehog; Indian hedgehog homolog; Indian hedgehog protein; Indian hedgehog protein C-product; Indian hedgehog protein N-product
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    E.Coli
  • 分子量：
    19.8 kDa
  • 表达区域：
    29-202aa
  • 氨基酸序列
    II+GPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG
  • 蛋白标签：
    Tag-Free
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from a 0.2 μm filtered 1 × PBS with 0.02% Tween-20, pH 7.0.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    5-10 business days
  • 产品描述：
    Recombinant Human Indian hedgehog protein (IHH) (Active) is a partial length protein expressed without tag in E.coli. Its expression region corresponds to 29-202aa of human IHH protein. Its purity was determined by SDS-PAGE and HPLC and reached up to 96%. It is presented a molecular mass band of 19.8 kDa on the gel. The endotoxin of this IHH protein is less than 1.0 EU/ug measured by the LAL method. It was also identified as an active protein through a functional ELISA. This recombinant IHH protein may be used to synthesize antibodies against IHH or on the studies of IHH-associated signal transduction. IHH proteins in-stock are available.
    IHH is a member of the hedgehog family of proteins which are essential secreted signaling molecules. These signaling molecules regulate a variety of developmental processes including growth, patterning and morphogenesis. And IHH specifically plays a role in bone growth and differentiation. IHH mutations are the cause of brachydactyly type A1, which is characterized by shortening or malformation of the fingers and toes.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).
  • 基因功能参考文献：
    1. TL1A modulated Rheumatoid arthritis-fibroblast-like synoviocytes migration and Indian hedgehog signaling pathway using TNFR2. PMID: 29748156
    2. Association of pathogenic variants in IHH with short stature with nonspecific skeletal abnormalities and a frequent cause of growth disorder, with a preliminary good response to rhGH. PMID: 29155992
    3. Studies suggest significance of other signaling aside from hedgehog in the pathogenesis of basal cell carcinoma (BCC) of the skin. PMID: 28574612
    4. Gorlin syndrome-derived induced pluripotent stem cells (iPSCs) expressed lower basal levels than control iPSCs of the genes encoding the Hh ligands Indian Hedgehog (IHH) and Sonic Hedgehog (SHH). PMID: 29088246
    5. Hedgehog pathway activation in T-cell acute lymphoblastic leukemia predicts response to SMO and GLI1 inhibitors. PMID: 27694322
    6. CLIC4 and Ihh could serve as biological markers for the progression, metastasis and/or invasiveness of pancreatic ductal adenocarcinoma. PMID: 28205343
    7. The serum levels of IHH and SHH were significantly higher in autistic subjects than those of control subjects. The findings support a correlation between SHH, IHH and BDNF in autistic children, suggesting their pathological role in autism. PMID: 26691363
    8. Our results show for the first time that Indian hedgehog does not cause extracellular matrix degradation in healthy ex vivo cartilage or in the presence of IL-1beta PMID: 26705100
    9. The Annexin a2 Promotes Development in Arthritis through Neovascularization by Amplification Hedgehog Pathway. PMID: 26963384
    10. Studies indicate that the hedgehog (Hh) signaling pathway has become one of the most studied potential therapeutic targets in hematological malignancies. PMID: 24991722
    11. Findings demonstrated that Ihh promotes human cartilage endplate degeneration. PMID: 25958162
    12. endogenously produced IHH is playing a critical role in regulating hBMSC chondrogenesis. PMID: 25519748
    13. Duplication of the upstream IHH regulatory region and a correlation between the phenotype and the implicated regulatory regions in a family with craniosynostosis Philadelphia type. PMID: 25692887
    14. Data show that the Hh (Hedgehog) signalling pathway mediates chemoresistance of BCSCs (breast cancer stem cells) and contributes to the outcome in breast cancer patients. PMID: 26201092
    15. Data suggest that hedgehog (HH) signaling through transcription factors GLI1 and GLI2 could be required for the maintenance of pregnancy. PMID: 25888497
    16. Studies indicate that activation of the hedgehog (Hh) signaling pathway has been observed in several cancer types, including ovarian cancer. PMID: 23303278
    17. Gene transfer of indian hedgehog protein results in chondrogenic induction of mesenchymal stem cells. PMID: 22817660
    18. Data indicate that neutralizing hedgehog antibody MEDI-5304 represents a potent dual hedgehog inhibitor suitable for patients with tumors harboring elevated levels of sonic hedgehog (SHH) or indian hedgehog (IHH). PMID: 24344235
    19. Indian Hedgehog, a critical modulator in osteoarthritis, could be a potential therapeutic target for attenuating cartilage degeneration disease. PMID: 24844414
    20. Increased Ihh is associated with the severity of osteoarthritis cartilage damage. Elevated Ihh content in human knee joint synovial fluid correlates with early cartilage lesions. PMID: 24786088
    21. Data indicate that overexpression of Indian hedgehog (Ihh) signaling promotes abnormal chondrocyte differentiation in enchondral ossification and enhances bone formation in cervical ossification of the posterior longitudinal ligament (OPLL). PMID: 23883825
    22. We further indicated that SHH and IHH were highly expressed in metastatic tumors PMID: 23468532
    23. Studies indicate that upregulation of Hedgehog protein has been found in tumor-associated stroma and associated with vascularization and excessive stromal expansion can limit intratumoral drug delivery. PMID: 23575478
    24. Hedgehog signaling regulates hypoxia induced epithelial to mesenchymal transition and invasion in pancreatic cancer cells via a ligand-independent manner. PMID: 23786654
    25. Data indicate that compounds 7 and 9 (diterpene) showed cytotoxicity against cancer cell lines in which hedgehog (Hh) signaling was aberrantly activated. PMID: 23403897
    26. Data indicate that analysis of public microarray data suggests that PBOV1 activation in tumors could be dependent on the Hedgehog signaling pathway. PMID: 23418531
    27. Results suggest that hypoxia-induced increase of Smo directly contributes to the proliferation of pancreatic ductal adenocarcinoma (PDAC) cells through a hedgehog/Gli1-independent pathway. PMID: 22486854
    28. Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that enhances expression of type II collagen. PMID: 22750747
    29. A missense mutation in IHH gene is observed in an individual affected by brachydactyly type A1. PMID: 22406540
    30. A large duplication involving the IHH locus mimics acrocallosal syndrome PMID: 22234151
    31. Activation of Indian hedgehog promotes chondrocyte hypertrophy and upregulation of MMP-13 in human osteoarthritic cartilage. PMID: 22469853
    32. Ihh-induced Nkx3.2 degradation requires Wnt5a, which is capable of triggering Nkx3.2 degradation PMID: 22507129
    33. Studies indicate that pathways of Hedgehog (Hh), Wnt and Notch, which regulate development during embryonic life and somatic stem cells (SCs) in the adult organism, can be reactivated in malignancies and support tumor-initiating cells (TIC) scompartment. PMID: 22123293
    34. Studies indicate that IHH is required for normal chondrocyte proliferation during both embryonic and postnatal growth. PMID: 21642379
    35. Nuclear and cytoplasmic endometrial expression of Indian hedgehog is decreased in women with endometriosis. PMID: 21640343
    36. VLDL carries Ihh throughout the body in mammals PMID: 20839884
    37. the observed duplications lead to a misexpression and/or overexpression of IHH and by this affect the complex regulatory signaling network during digit and skull development. PMID: 21167467
    38. This data confirms genetic heterogeneity in Brachydactyly A1 (BDA1) and demonstrates that mutations upstream of IHH can result in BDA1. PMID: 20683927
    39. The temporal increase in endometrial IHH during the secretory phase, and their modulation by CDB-2914 suggests progestin role in endometrial differentiation and implantation. PMID: 20881264
    40. down-regulation of indian hedgehog expression as a result of hypermethylation, may be an early event in colorectal carcinogenesis PMID: 20854074
    41. Methylation analysis revealed that IHH promoter was hypermethylated in colon cancer cell lines. PMID: 19856079
    42. Indian hedgehog protein was detected in cystic tubules within human dysplastic kidneys; these molecules modify severity of this congenital malformation. Hedgehog signaling may be important stimulus for renal cyst formation. PMID: 20007344
    43. describes cloning of human IHH PMID: 7720571
    44. Data show that all of the BDA1 mutations occur in a restricted area of the N-terminal active fragment of the IHH. PMID: 19277064
    45. expression is preserved in the growth plate of human fetuses affected with fibroblast growth factor receptor type 3 activating mutations PMID: 12368206
    46. novel mutation causes brachydactyly type A1 PMID: 12384778
    47. A novel missense mutation in the IHH gene mapping to chromosome 5 has been identified identified in all patients of a brachydactyly type A1 family. PMID: 12525541
    48. Homozygous mutations in this gene cause acrocapitofemoral dysplasia, an autosomal recessive disorder with cone-shaped epiphyses in hands and hips. PMID: 12632327
    49. model of the interactions between beta-catenin and hedgehog signaling in the epidermis in which SHH promotes proliferation of progenitors of the hair lineages whereas IHH stimulates proliferation of sebocyte precursors PMID: 12917489
    50. a wide range of digestive tract tumours, including most of those originating in the oesophagus, stomach, biliary tract and pancreas, but not in the colon, display increased Hh pathway activity, which is suppressible by cyclopamine, a Hh pathway antagonist PMID: 14520411

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  • 相关疾病：
    Brachydactyly A1 (BDA1); Acrocapitofemoral dysplasia (ACFD)
  • 亚细胞定位：
    [Indian hedgehog protein N-product]: Cell membrane; Lipid-anchor; Extracellular side.; [Indian hedgehog protein C-product]: Secreted, extracellular space.; Cell membrane.
  • 蛋白家族：
    Hedgehog family
  • 组织特异性：
    Expressed in embryonic lung, and in adult kidney and liver.
  • 数据库链接：

    HGNC: 5956

    OMIM: 112500

    KEGG: hsa:3549

    STRING: 9606.ENSP00000295731

    UniGene: Hs.654504