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  4. Rabbit Growth Hormone(GH) ELISA Kit

Rabbit Growth Hormone(GH) ELISA Kit

  • 中文名称：
    兔子生长激素(GH)酶联免疫试剂盒
  • 货号：
    CSB-E15021Rb
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
    CUSABIO定量检测兔子生长激素（GH）的酶联免疫试剂盒（货号：CSB-E15021Rb），采用竞争法检测原理，适用于血清、血浆及组织匀浆样本中GH含量的精准分析。生长激素是由垂体前叶分泌的多肽类激素，在动物生长发育、代谢调节及组织修复中发挥重要作用，科研中常用于探究营养代谢、生长发育调控及内分泌相关疾病模型的机制研究。该试剂盒检测范围为6.25-100 ng/mL，灵敏度高，可在低浓度样本中稳定检出目标蛋白。试剂盒包含预包被抗体的微孔板、标准品、检测抗体及显色底物等核心组分，操作流程包括样本孵育、竞争结合、洗涤和显色步骤，3-4小时内即可完成检测。其适用于科研领域如动物生理学研究、GH相关基因表达调控实验、药物或营养干预对激素水平影响的评估，以及兔科动物疾病模型（如垂体功能异常）的机制探索，为实验室提供高效、可靠的GH定量分析工具。
  • 别名：
    GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 ng/mL-100 ng/mL
  • 灵敏度：
    6.25 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 89    
    Range % 82-97    
    1:2 Average % 95    
    Range % 89-100    
    1:4 Average % 90    
    Range % 85-100    
    1:8 Average % 92    
    Range % 89-102    
  • 回收率：
    The recovery of rabbit GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 96 90-110    
    EDTA plasma (n=4) 95 89-100    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average 　    
    100 0.169 0.171 0.170 　    
    50 0.270 0.276 0.273 　    
    25 0.496 0.488 0.492 　    
    12.5 1.033 1.051 1.042 　    
    6.25 2.062 2.053 2.058 　    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 数据库链接：