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重组蛋白

产品名称 货号 规格
Recombinant Deinococcus geothermalis NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP628670DAAD
CSB-EP628670DAAD
CSB-BP628670DAAD
CSB-MP628670DAAD
CSB-EP628670DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis Translation initiation factor IF-2 (infB), partial CSB-YP628671DAAD
CSB-EP628671DAAD
CSB-BP628671DAAD
CSB-MP628671DAAD
CSB-EP628671DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis Phosphoglycerate kinase (pgk) CSB-YP628672DAAD
CSB-EP628672DAAD
CSB-BP628672DAAD
CSB-MP628672DAAD
CSB-EP628672DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP628673DAAD
CSB-EP628673DAAD
CSB-BP628673DAAD
CSB-MP628673DAAD
CSB-EP628673DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis Elongation factor Ts (tsf) CSB-YP628674DAAD
CSB-EP628674DAAD
CSB-BP628674DAAD
CSB-MP628674DAAD
CSB-EP628674DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis Gamma-glutamyl phosphate reductase (proA) CSB-YP628675DAAD
CSB-EP628675DAAD
CSB-BP628675DAAD
CSB-MP628675DAAD
CSB-EP628675DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP628676DAAD
CSB-EP628676DAAD
CSB-BP628676DAAD
CSB-MP628676DAAD
CSB-EP628676DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP628677DAAD
CSB-EP628677DAAD
CSB-BP628677DAAD
CSB-MP628677DAAD
CSB-EP628677DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP628678DAAD
CSB-EP628678DAAD
CSB-BP628678DAAD
CSB-MP628678DAAD
CSB-EP628678DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis DNA ligase (ligA), partial CSB-YP628679DAAD
CSB-EP628679DAAD
CSB-BP628679DAAD
CSB-MP628679DAAD
CSB-EP628679DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis 50S ribosomal protein L7/L12 (rplL) CSB-YP628680DAAD
CSB-EP628680DAAD
CSB-BP628680DAAD
CSB-MP628680DAAD
CSB-EP628680DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP628681DAAD
CSB-EP628681DAAD
CSB-BP628681DAAD
CSB-MP628681DAAD
CSB-EP628681DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP628682DAAD
CSB-EP628682DAAD
CSB-BP628682DAAD
CSB-MP628682DAAD
CSB-EP628682DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis Protoheme IX farnesyltransferase (ctaB), partial CSB-YP628683DAAD
CSB-EP628683DAAD
CSB-BP628683DAAD
CSB-MP628683DAAD
CSB-EP628683DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis S-adenosylmethionine synthase (metK) CSB-YP628684DAAD
CSB-EP628684DAAD
CSB-BP628684DAAD
CSB-MP628684DAAD
CSB-EP628684DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP628685DAAD
CSB-EP628685DAAD
CSB-BP628685DAAD
CSB-MP628685DAAD
CSB-EP628685DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus geothermalis Phosphate acetyltransferase (pta), partial CSB-YP628686DAAD
CSB-EP628686DAAD
CSB-BP628686DAAD
CSB-MP628686DAAD
CSB-EP628686DAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M4 Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP628687SAAW
CSB-EP628687SAAW
CSB-BP628687SAAW
CSB-MP628687SAAW
CSB-EP628687SAAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M4 DNA mismatch repair protein MutS (mutS), partial CSB-YP628688SAAW
CSB-EP628688SAAW
CSB-BP628688SAAW
CSB-MP628688SAAW
CSB-EP628688SAAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M4 30S ribosomal protein S2 (rpsB) CSB-YP628689SAAW
CSB-EP628689SAAW
CSB-BP628689SAAW
CSB-MP628689SAAW
CSB-EP628689SAAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用资源

什么是重组蛋白?

重组蛋白是一种可操纵的蛋白质形式,可以通过多种方式产生大量蛋白质、修改基因序列和制造有用的商业产品。重组蛋白的技术资源在重组蛋白的开发过程中,除了表达系统之外,还面临着一系列令人困惑的选择,如表达载体的选择、表达蛋白的长度(全长或部分)、是否带标签等。在生产重组蛋白时,你必须做出很多决定。如果你选择的明智,你将获得高质量的重组蛋白,后续的实验更有可能成功。但是如果你做了错误的决定,你可能得不到你所需要的重组蛋白或者重组蛋白的质量和纯度不符合要求。在这里,来自CUSABIO的蛋白表达工程师根据他们开发重组蛋白的经验总结出了几个有用的提示,可以帮助你做出明智的决策。

第一条:重组蛋白的生产,在这篇文章中,我们旨在阐明重组蛋白的定义以及如何生产重组蛋白。

第二条:如何选择合适的表达载体?

表达载体是一种DNA分子,它携带特定的基因进入宿主细胞,并利用细胞的蛋白质合成机制来产生由该基因编码的蛋白质。表达载体可使外源基因在宿主细胞中高效表达,经过多年的发展,表达载体已成为一种成熟而受欢迎的生物技术。在重组蛋白生产过程中是一种基本成分。正如标题所示,本文主要总结了几个有用的技巧来选择合适的表达载体。

第三条:如何表达具有生物活性的蛋白质?

根据重组蛋白的不同应用,对其生物活性有不同的要求。但是我们如何确保蛋白质的产生具有生物活性呢?本文将围绕这个主题,并提供一些解决方案。除了以上文章,我们还收集了一些重组蛋白的常见问题。您可以单击以下链接查看。/technology-91.html。此外,我们还为我们的客户提供重组蛋白表达服务,您可以点击这里获取更多信息。注:如果您对CUSABIO的蛋白质有任何疑问,涉及到蛋白质的价格、交货、质量等,您可以点击橙色按钮为我们在线留言。

引用资料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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