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  4. Recombinant Rat Thioredoxin (Txn)

Recombinant Rat Thioredoxin (Txn)

  • 货号：
    CSB-YP025365RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025365RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025365RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025365RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025365RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Txn; Txn1; Thioredoxin; Trx
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-105
  • 氨基酸序列
    VKLIESKEA FQEALAAAGD KLVVVDFSAT WCGPCKMIKP FFHSLCDKYS NVVFLEVDVD DCQDVAADCE VKCMPTFQFY KKGQKVGEFS GANKEKLEAT ITEFA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

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靶点详情

  • 功能：
    Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
  • 基因功能参考文献：
    1. A delay in tumor formation and a lower cancerization rate was observed following the inhibition of Trx-1 with an irreversible inhibitor in the rat squamous cell carcinoma model. PMID: 29328386
    2. Data suggest that thioredoxin-1 (Trx1) modulates activity of soluble nitric oxide-sensitive guanylyl cyclase (GC1, subunits alpha and beta) through an association between Trx1 and GC1 via a mixed disulfide; Trx1 protects GC1 from desensitization. These studies were conducted, in part, using rat neonatal cardiomyocytes in primary culture. PMID: 28659344
    3. Trx1 exerts its neuroprotective effects by preventing oxidative stress in astrocytes via maintaining Prdx expression. PMID: 26846911
    4. Ginsenoside Rb1 shows a potent anti-oxidative effect on cultured PC12 cells by inducing Trx-1 expression. PMID: 26346161
    5. expression increased in cold adaptation PMID: 26327811
    6. Results suggest that Trx1 siRNA increases apoptotic stress-induced ASK1 activation and this represents further evidence that Trx1 is an endogenous anti-apoptotic molecule that diminishes focal cerebral ischemia/reperfusion injury PMID: 25541364
    7. both type 1 and type 2 diabetic rats demonstrated a significant increase in TxnIP mRNA and reduction in thioredoxin activity when compared to non-diabetic animals. PMID: 24925443
    8. Reduced form of Trx is required for cerebellar granule cell survival. PMID: 25055978
    9. Mycophenolic acid reduces the expression of Trx1 at the protein and gene levels, leading to the apoptosis of insulin-secreting cells via the MKK4/7-JNK pathway. PMID: 23846223
    10. Data indicate that Trx-1 is a therapeutic candidate for protecting against formaldehyde-induced neurotoxicity. PMID: 22342837
    11. the wave-like oscillations of Trx-1 expression during hypobaric hypoxia preconditioning was described in hypothalamus. PMID: 22165200
    12. Data suggest that Trx1 decreases in optic nerves and isolated retinal ganglion cells (RGC) after exposure to tumor necrosis factor-alpha in models of optic nerve degeneration with subsequent loss of RGC; 17beta-estradiol regulates expression of Trx1. PMID: 21586560
    13. Data provide implication of the Trx1/Txnip system in hepatic I/R injuries. PMID: 20709139
    14. respiration- and thioredoxin-dependent ROS removal as a potentially important mitochondrial function that may contribute to physiological and pathological processes in the brain PMID: 20558743
    15. Thioredoxin may mediate the neuroprotection conferred by estradiol against iron-induced brain injury in vivo. PMID: 19940280
    16. vitamin D(3)-up-regulated protein-1/thioredoxin axis has an important role in the preservation of cellular viability PMID: 12011048
    17. Activation of thioredoxin participates in the development of pressure-overload cardiac hypertrophy, demonstrating the dual function of thioredoxin as both an antioxidant and a signaling protein. PMID: 15123525
    18. reduced thioredoxin activity through interaction with Txnip is an important mechanism for vascular oxidative stress in diabetes mellitus PMID: 15128745
    19. AlphaAR-stimulated hypertrophic signaling in adult rat ventricular myocytes is mediated via a TRX1-sensitive posttranslational oxidative modification of thiols on Ras. PMID: 15723974
    20. during thioredoxin-dependent enzymatic activation of mercaptopyruvate sulfurtransferase, an intersubunit disulfide bond serves as a redox switch for activation PMID: 17130129
    21. Our findings indicate that cholestasis is associated with significant mutual and interrelated changes between circulating and hepatic thioredoxin and nitrosothiols. PMID: 17256724
    22. AngII induces thioredoxin in neuronal cultures. Thioredoxin then negatively regulates AngII stimulated neuronal activity via modulation of delayed rectifier K+ currents. PMID: 17360810
    23. Diabetes impairs exercise training-associated thioredoxin response and glutathione status in rat brain. PMID: 19074570

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Secreted.
  • 蛋白家族：
    Thioredoxin family
  • 数据库链接：

    KEGG: rno:116484

    STRING: 10116.ENSRNOP00000016447

    UniGene: Rn.29777