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  4. Recombinant Human Lymphocyte antigen 6 complex locus protein G6d (LY6G6D) (Active)

Recombinant Human Lymphocyte antigen 6 complex locus protein G6d (LY6G6D) (Active)

In Stock
  • 货号：
    CSB-YP013246HU
  • 规格：
    ￥1500
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized Human LY6G6D at 2 μg/mL can bind Anti-LY6G6D recombinant antibody (CSB-RA013246MA1HU), the EC50 is 2.816-3.741 ng/mL.
  • 其他：

产品详情

  • 纯度：
    Greater than 95% as determined by SDS-PAGE.
  • 内毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    Measured by its binding ability in a functional ELISA. Immobilized Human LY6G6D at 2 μg/mL can bind Anti-LY6G6D recombinant antibody (CSB-RA013246MA1HU), the EC50 is 2.816-3.741 ng/mL.
  • 基因名：
  • Uniprot No.：
  • 别名：
    (Protein Ly6-D)(Megakaryocyte-enhanced gene transcript 1 protein)
  • 分子结构：
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    11.1 kDa
  • 表达区域：
    20-104aa
  • 氨基酸序列
    NRMRCYNCGGSPSSSCKEAVTTCGEGRPQPGLEQIKLPGNPPVTLIHQHPACVAAHHCNQVETESVGDVTYPAHRDCYLGDLCNS
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    This Human LY6G6D recombinant protein was produced in Yeast, where the gene sequence encoding Human LY6G6D (20-104aa) was expressed with the N-terminal 6xHis tag. The purity of this LY6G6D protein was greater than 95%. The activity was validated.

    LY6G6D gene encodes a protein of 133 amino acid residues with a molecular weight of 13.7 kDa and an isoelectric point of 6.56. The N-terminal 19 amino acid residues are the signal peptide, and the mature protein is composed of 85 amino acid residues. By bioinformatics analysis, the protein has a Ly-6/UPAR-like domain, which starts with leucine or isoleucine as the N-terminal and has 8-10 conserved cysteines. It is a member of the Ly-6 superfamily of glycosylphosphatidylinositol (GPI)-anchored cell surface proteins, and the 104th amino acid is the GPI anchoring site.
    LY6G6D may be related to the occurrence of tumors, the characteristics of tumor stem cells, and the metastasis of tumor cells. However, the signaling pathways and mechanisms of LY6G6D in tumors need further research and exploration. It is expected that LY6G6D can be used as a potential new biological therapeutic target to provide new theoretical basis and methods for tumor treatment research.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

平均分:
5.0分 - 1 个评价

实验应用：药物相关研究, 体外酶活性检测

产品评价： 我们使用CSB-YP013246HU产品，与抗LY6G6D的抗体进行ELISA，EC50 为4.162-5.164 ng/ml，有较好的结合活性。后续用于药物高通量筛选，有较好的亲和力。产品活性不错，实验进展很顺利！会推荐给同实验组的师弟师妹们，强烈安利！

By 张老师

问答及客户评论

■ 常见问题解答
Q:

Our questions are the following:
1)Both baculovirus and yeast expressed proteins are N-terminal His tagged. However we can only detect the His tag from the baculovirus expressed but not the yeast expressed. Were there any attempt try to remove the his tag?
2)My second question is that are these protein constructs designed as secretory soluble proteins? If so, what was the signal peptides used since the native signal peptides were removed?
3)Have there been any validation attempts to look at if the proteins are folded correctly, e.g. using LY6G6D antibodies? Was there any other product validation steps performed?

A:
Thanks for your inquiry.
1. We haven't tried to remove the tag at present. CSB-YP013246HU indeed is N-terminal His tagged. We are also very surprised that you can not detect the tag.
2. Yes. These protein constructs designed as secretory soluble proteins, but we use the signal peptide that comes with the vectors. The vectors contain signal peptide restriction sites.
We're worry but we can't provide further information because info are confidential.
3. Eukaryotic expression has a post-translational modification which can be correctly folded in theory, but we haven't tested the protein structure yet.
For CSB-YP013246HU, we will test it using His-tag-antibody. It will take 3-5 working days to finish the experiment. We will share the result with you once we finish it.

靶点详情

  • 基因功能参考文献：
    1. Frequencies of the DRB1, DQA1, DQB1 and TNFA alleles in immigrant population of West Siberia PMID: 19807019
  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor. Cell projection, filopodium.
  • 组织特异性：
    Expressed in the adult lung, and in fetal liver, lung, kidney, brain and spleen.
  • 数据库链接：

    HGNC: 13935

    OMIM: 606038

    KEGG: hsa:58530

    STRING: 9606.ENSP00000364985

    UniGene: Hs.591792