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  4. Rat Thioredoxin,Trx ELISA Kit

Rat Thioredoxin,Trx ELISA Kit

  • 中文名称：
    大鼠硫氧化还原蛋白(Trx)酶联免疫试剂盒
  • 货号：
    CSB-E09729r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat Thioredoxin (Trx) ELISA Kit is a highly sensitive and specific assay for the quantitative detection of Trx in serum, plasma, and tissue homogenates from the Rat species.

    Trx is a multifunctional protein that plays a vital role in maintaining cellular redox balance by acting as a reducing agent. Abnormal Trx levels have been linked to a variety of pathological conditions, including oxidative stresses，cancer, neurodegenerative diseases and cardiovascular disorders. This Trx ELISA Kit offers a reliable and efficient means of measuring Trx levels in biological samples, with a detection range of 3.12 ng/mL to 200 ng/mL and a sensitivity of 0.78 ng/mL.

    The assay principle of our Trx ELISA Kit is based on the sandwich method. During the assay process, Trx in the sample is sandwiched between two Trx-specific antibodies, which are coated on the plate. The bound Trx is then detected using a detection antibody conjugated with HRP and a chromogenic substrate, producing a colorimetric signal that is directly proportional to the Trx concentration.

    Our Trx ELISA Kit offers a quick assay time of 1-5 hours, with a sample volume of 50-100ul and a detection wavelength of 450 nm. It is suitable for use in metabolism and oxidative stresses research,has been optimized for use with Rat samples. Its quantitative assay principle, coupled with its wide detection range and high sensitivity, make it an invaluable tool for researchers studying the physiological and pathological roles of Trx in cellular metabolism.

  • 别名：
    Txn ELISA Kit; Txn1 ELISA Kit; Thioredoxin ELISA Kit; Trx ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Trx in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %98
    Range %94-102
    1:2Average %91
    Range %86-97
    1:4Average %90
    Range %85-94
    1:8Average %94
    Range %90-100
  • 回收率：
    The recovery of rat Trx spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10297-109
    EDTA plasma (n=4)9691-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1OD2AverageCorrected
    2002.412 2.312 2.362 2.242
    1001.722 1.632 1.677 1.557
    501.168 1.193 1.181 1.061
    250.726 0.749 0.738 0.618
    12.50.480 0.451 0.466 0.346
    6.250.286 0.275 0.281 0.161
    3.120.229 0.214 0.222 0.102
    00.122 0.118 0.120
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
  • 基因功能参考文献：
    1. A delay in tumor formation and a lower cancerization rate was observed following the inhibition of Trx-1 with an irreversible inhibitor in the rat squamous cell carcinoma model. PMID: 29328386
    2. Data suggest that thioredoxin-1 (Trx1) modulates activity of soluble nitric oxide-sensitive guanylyl cyclase (GC1, subunits alpha and beta) through an association between Trx1 and GC1 via a mixed disulfide; Trx1 protects GC1 from desensitization. These studies were conducted, in part, using rat neonatal cardiomyocytes in primary culture. PMID: 28659344
    3. Trx1 exerts its neuroprotective effects by preventing oxidative stress in astrocytes via maintaining Prdx expression. PMID: 26846911
    4. Ginsenoside Rb1 shows a potent anti-oxidative effect on cultured PC12 cells by inducing Trx-1 expression. PMID: 26346161
    5. expression increased in cold adaptation PMID: 26327811
    6. Results suggest that Trx1 siRNA increases apoptotic stress-induced ASK1 activation and this represents further evidence that Trx1 is an endogenous anti-apoptotic molecule that diminishes focal cerebral ischemia/reperfusion injury PMID: 25541364
    7. both type 1 and type 2 diabetic rats demonstrated a significant increase in TxnIP mRNA and reduction in thioredoxin activity when compared to non-diabetic animals. PMID: 24925443
    8. Reduced form of Trx is required for cerebellar granule cell survival. PMID: 25055978
    9. Mycophenolic acid reduces the expression of Trx1 at the protein and gene levels, leading to the apoptosis of insulin-secreting cells via the MKK4/7-JNK pathway. PMID: 23846223
    10. Data indicate that Trx-1 is a therapeutic candidate for protecting against formaldehyde-induced neurotoxicity. PMID: 22342837
    11. the wave-like oscillations of Trx-1 expression during hypobaric hypoxia preconditioning was described in hypothalamus. PMID: 22165200
    12. Data suggest that Trx1 decreases in optic nerves and isolated retinal ganglion cells (RGC) after exposure to tumor necrosis factor-alpha in models of optic nerve degeneration with subsequent loss of RGC; 17beta-estradiol regulates expression of Trx1. PMID: 21586560
    13. Data provide implication of the Trx1/Txnip system in hepatic I/R injuries. PMID: 20709139
    14. respiration- and thioredoxin-dependent ROS removal as a potentially important mitochondrial function that may contribute to physiological and pathological processes in the brain PMID: 20558743
    15. Thioredoxin may mediate the neuroprotection conferred by estradiol against iron-induced brain injury in vivo. PMID: 19940280
    16. vitamin D(3)-up-regulated protein-1/thioredoxin axis has an important role in the preservation of cellular viability PMID: 12011048
    17. Activation of thioredoxin participates in the development of pressure-overload cardiac hypertrophy, demonstrating the dual function of thioredoxin as both an antioxidant and a signaling protein. PMID: 15123525
    18. reduced thioredoxin activity through interaction with Txnip is an important mechanism for vascular oxidative stress in diabetes mellitus PMID: 15128745
    19. AlphaAR-stimulated hypertrophic signaling in adult rat ventricular myocytes is mediated via a TRX1-sensitive posttranslational oxidative modification of thiols on Ras. PMID: 15723974
    20. during thioredoxin-dependent enzymatic activation of mercaptopyruvate sulfurtransferase, an intersubunit disulfide bond serves as a redox switch for activation PMID: 17130129
    21. Our findings indicate that cholestasis is associated with significant mutual and interrelated changes between circulating and hepatic thioredoxin and nitrosothiols. PMID: 17256724
    22. AngII induces thioredoxin in neuronal cultures. Thioredoxin then negatively regulates AngII stimulated neuronal activity via modulation of delayed rectifier K+ currents. PMID: 17360810
    23. Diabetes impairs exercise training-associated thioredoxin response and glutathione status in rat brain. PMID: 19074570

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Secreted.
  • 蛋白家族：
    Thioredoxin family
  • 数据库链接：