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  4. Recombinant Mouse Mitogen-activated protein kinase 3 (Mapk3)

Recombinant Mouse Mitogen-activated protein kinase 3 (Mapk3)

  • 货号：
    CSB-YP013456MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013456MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP013456MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013456MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013456MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mapk3; Erk1; Prkm3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; EC 2.7.11.24; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; MNK1; Microtubule-associated protein 2 kinase; p44-ERK1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-380
  • 氨基酸序列
    AAAAAAPGG GGGEPRGTAG VVPVVPGEVE VVKGQPFDVG PRYTQLQYIG EGAYGMVSSA YDHVRKTRVA IKKISPFEHQ TYCQRTLREI QILLRFRHEN VIGIRDILRA PTLEAMRDVY IVQDLMETDL YKLLKSQQLS NDHICYFLYQ ILRGLKYIHS ANVLHRDLKP SNLLINTTCD LKICDFGLAR IADPEHDHTG FLTEYVATRW YRAPEIMLNS KGYTKSIDIW SVGCILAEML SNRPIFPGKH YLDQLNHILG ILGSPSQEDL NCIINMKARN YLQSLPSKTK VAWAKLFPKS DSKALDLLDR MLTFNPNKRI TVEEALAHPY LEQYYDPTDE PVAEEPFTFD MELDDLPKER LKELIFQETA RFQPGAPEGP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
  • 基因功能参考文献：
    1. Low ERK1 Phosphorylation is associated with low liver regeneration. PMID: 30142376
    2. A. fumigatus increased PAR-2 expression and elevated disease, PMN infiltration, and proinflammatory cytokine expreERK1 Kinasession through PAR-2, which may be modified by p-ERK1/2. PMID: 29332129
    3. ERK1 and ERK2 play specific roles in beta cells. ERK2 cannot always compensate for the lack of ERK1 but the absence of a clear-cut phenotype in Erk1 (-/-) mice shows that ERK1 is dispensable in normal conditions. PMID: 28721437
    4. The Macrophage Activation Induced by Bacillus thuringiensis Cry1Ac Protoxin Involves ERK1/2 and p38 Pathways and the Interaction with Cell-Surface-HSP70. PMID: 28608568
    5. hBD-1 potentiates the induction of in vitro osteoclastogenesis by RANKL via enhanced phosphorylation of the p44/42 MAPKs. PMID: 28709835
    6. ERK1 role in the osteoclast differentiation.Insulin induces RANK expression via ERK1/2, which contributes to the enhancement of osteoclast differentiation. PMID: 28535663
    7. ERK1 underexpression is associated with obesity. PMID: 28302472
    8. We also demonstrated that ERK1 deletion potentiates DC vaccination and provides a survival advantage in high-grade gliomas. PMID: 27256374
    9. Consistent with the essential role of ERK1/2 during macrophage differentiation and defects of macrophagic differentiation in native ERK1(-/-) progenitors, ERK signaling is strongly activated in Eger/Fms cells upon M-CSF-induced macrophage differentiation. PMID: 26336156
    10. T1R1/T1R3 have roles in regulating ERK1/2 and mTORC1 in MIN6 cells PMID: 26168033
    11. ERK/GSK3beta signaling was involved in the apoptotic and G1 phase arrest effects of AT-I in melanoma cells. PMID: 26151480
    12. RET coupling to ERK and Akt depends strongly on artemin concentration, and it is highest at the low (~100 pM) artemin levels required for neurite outgrowth. PMID: 25635057
    13. ablates negative regulation by AMPK and contributes to FcepsilonRI-mediated activation of the mast cells PMID: 24948367
    14. our data implicate MNK1 as a key component of BDNF-mediated translational regulation in neurons. PMID: 25609615
    15. The rapidly changing ERK1 phosphorylation was visualized in the intestinal epithelial stem cells of mouse small intestine. PMID: 23793953
    16. Data indicate that caspase-8 activity is lost upon deletion of c-FLIPL, and p43FLIP rescues caspase-8 activity through Raf1, TRAF2, and RIPK1 association, augmenting ERK and NF-kappaB pathways. PMID: 24275659
    17. The observed activation ERK1/2 and GFAP, both markers of Muller cell gliosis, in the absence of Muller cell proliferation, is consistent with the activation of atypical gliosis occurring during the slow process of degeneration in Rpe65(-/-) mice. PMID: 24012986
    18. The findings of this study suggested a model where ERK1/2 MAP kinase signaling acts as a myelin thickness rheostat that instructs oligodendrocytes to generate axon-appropriate quantities of myelin. PMID: 24259565
    19. As the retina matures, ERK1 is activated and plays a role in retinal degeneration. PMID: 20950336
    20. desipramine-increased HO-1 expression is mediated by Nrf2 activation through the ERK and JNK signaling pathways PMID: 23209658
    21. Data indicate that thiazolidinediones (TZDs) rapidly activated Erk1/2 and p38 through G protein-coupled receptor 40 (GPR40), a receptor expressed on the surface of osteocytes. PMID: 22593579
    22. Development of bladder hyperalgesia was associated with robust enhancement of ERK1/2 activation in the dorsal horn and deeper laminae bilaterally in the L6-S1 spinal cord. PMID: 21705143
    23. IL-4 may protect mice against BCG/LPS-induced immune liver injury, with ERK and NF-kappaB signal pathways involved in the effects PMID: 21947361
    24. MAPK phosphorylation is a useful marker to study short-term neural activation associated with the expression of specific behaviors. PMID: 21451356
    25. Data demonstrate Erk1 plays an important role in osteoclast functions. PMID: 21961044
    26. ERK1/2 was stimulated in neurons cultured from mucopolysaccharidosis type IIIB mice. ERK1/2 was significantly higher in the cortex of 1-2-month-old affected animals compared with wild-type littermates. PMID: 21618584
    27. For the first time, activation of ERK 1/2 in the spinal cord reveals the time-wise development of an estrogen-dependent chronic hyperalgesic state. PMID: 20971161
    28. An aberrant ERK1/2 signaling mechanism influences various neuronal functions and plays a significant role in cognitive dysfunction of ribosomal s6 kinase (rsk)2 deficient mice. PMID: 21838783
    29. dopamine D1 receptor agonists cause Erk-dependent increases in Zif268 and Arc/Arg3.1 expression in mouse dentate gyrus PMID: 21559295
    30. identify ERK1 as an important regulator of PrP(c) cellular homeostasis and indicate that this kinase exerts a dual control of PrP(c) levels through transcriptional and post-transcriptional mechanisms. PMID: 21586567
    31. Gene expression analysis showed a significant regulation of extracellular signal-regulated kinases (ERK)1, ERK2 and c-Jun N-terminal kinase (JNK)3 genes in JEV infected neuroblastoma cells. PMID: 21320173
    32. Data show that inhibition of MEK broadly affects the cellular network in a multipathway manner. PMID: 21427409
    33. Results demonstrate that the targeting of ERK1 could partially protect obese mice against insulin resistance and liver steatosis by decreasing adipose tissue inflammation and by increasing muscle glucose uptake. PMID: 20953578
    34. ROS generation by J774A.1-MacCM is required for Akt and ERK1/2 signaling to promote 3T3-L1 preadipocyte survival. PMID: 21056559
    35. Results indicate that activated MAPK3 (& MAPK1) promotes cell proliferation and differentiation in the initial segment of the developing epididymis; in the adult, the role if MAPK3 (& MAPK1) switches to maintaining cell survival. PMID: 20650883
    36. Data show that deletion of Erk1/2 in Schwann cell precursors caused disrupted differentiation and marked hypomyelination of axons. PMID: 21220101
    37. AMPK-mediated PLD1 activation is required for (14)C-glucose uptake through ERK stimulation PMID: 20231899
    38. Hypersensitivity to the ERK1/2 pathway activation leads to excessive protein synthesis in the hippocampus of a model of fragile X syndrome. PMID: 21084617
    39. Data suggest that ERK1 phosphorylation is indispensable for the GD1a signal suppressing NOS2. PMID: 20589721
    40. intracellular RHAMM(Delta163) functions as an adaptor protein to control microtubule polymerization during interphase and mitosis as a result of localizing ERK1/2-MEK1 complexes to their tubulin-associated substrates PMID: 20558733
    41. the Gemini vitamin D analog BXL0124 inhibits the growth of ErbB2 overexpressing mammary tumors through regulating the ErbB2/AKT/ERK signaling pathways PMID: 20304052
    42. These results conclude that ERK1 plays a limited role in nociceptive sensitization in an animal model of pain. PMID: 20739576
    43. p62 is a crucial regulator of ERK1 in metabolism PMID: 20154642
    44. MAPK3 is a negative regulator of dendritic cells. MAPK3 is dispensable for growth & development, but it plays an essential role in DC that then maintain a moderate T-cell response during autoimmune reactions. PMID: 20186879
    45. Loss of ERK1 results in an enhanced splenic erythropoiesis. PMID: 20223923
    46. ERK nuclear translocation is dimerization-independent but controlled by the rate of phosphorylation PMID: 19920141
    47. a preformed signaling complex mediates GnRH-activated ERK phosphorylation of paxillin and FAK at focal adhesions in L beta T2 gonadotrope cells PMID: 19628583
    48. In the superficial laminae of the dorsal horn of the spinal cord there was an increase in expression of Erk-1 following surgery under remifentanil anesthesia. PMID: 19917879
    49. Thus we demonstrate a GR-dependent ERK-mediated pathway that modulates auditory function after RS and acoustic trauma. PMID: 19413500
    50. Based on a MEK1-derived peptide, we developed inhibitors of ERK activation in vitro and in vivo. PMID: 11756441

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Membrane, caveola. Cell junction, focal adhesion.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
  • 数据库链接：

    KEGG: mmu:26417

    STRING: 10090.ENSMUSP00000051619

    UniGene: Mm.8385