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  4. Recombinant Human Tumor necrosis factor-inducible gene 6 protein (TNFAIP6)

Recombinant Human Tumor necrosis factor-inducible gene 6 protein (TNFAIP6)

  • 货号：
    CSB-YP023959HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP023959HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-MP023959HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TNFAIP6
  • Uniprot No.：
  • 别名：
    Hyaluronate binding protein; Hyaluronate-binding protein; TNF alpha induced protein 6; TNF alpha-induced protein 6; TNF stimulated gene 6 protein; TNF-stimulated gene 6 protein; TNFAIP 6; Tnfaip6; TSG 6; TSG-6; TSG6_HUMAN; Tumor necrosis factor alpha induced protein 6; Tumor necrosis factor alpha inducible protein 6; Tumor necrosis factor alpha-induced protein 6; Tumor necrosis factor inducible gene 6 protein; Tumor necrosis factor stimulated gene 6 protein; Tumor necrosis factor-inducible gene 6 protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    18-277
  • 氨基酸序列
    WGF KDGIFHNSIW LERAAGVYHR EARSGKYKLT YAEAKAVCEF EGGHLATYKQ LEAARKIGFH VCAAGWMAKG RVGYPIVKPG PNCGFGKTGI IDYGIRLNRS ERWDAYCYNP HAKECGGVFT DPKQIFKSPG FPNEYEDNQI CYWHIRLKYG QRIHLSFLDF DLEDDPGCLA DYVEIYDSYD DVHGFVGRYC GDELPDDIIS TGNVMTLKFL SDASVTAGGF QIKYVAMDPV SKSSQGKNTS TTSTGNKNFL AGRFSHL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Accession no. : P98066
Tag : Fluorescent tag​ (GFP or FITC etc, inform me of possible Tag you can provide)
Desired size : minimum scale

A:
Thanks for your inquiry.
Recombinant Human Tumor necrosis factor-inducible gene 6 protein(TNFAIP6)
CSB-YP023959HU >> Yeast
CSB-EP023959HU >> E.coli
CSB-BP023959HU >> Baculovirus
CSB-MP023959HU >> Mammalian cell
Expression Region: 18-277aa; Full length of the mature protein.
Tag information:EP: N-terminal 6xHis-tagged; YP, BP, MP: Tag type will be determined during the manufacturing process.
We can try your desired tag preferentially if you have, but as we can't 100% guaranteed, if it's failed, we will provide the protein with other tag.
The success rate of expressing the protein with your desired tag is 95.25%.
Target protein sequence: (The whole complete sequence will include tag sequene, target protein sequence and any linker sequence)

WGFKDGIFHNSIWLERAAGVYHREARSGKYKLTYAEAKAVCEFEGGHLATYKQLEAARKIGFHVCAAGWMAKGRVGYPIVKPGPNCGFGKTGIIDYGIRLNRSERWDAYCYNPHAKECGGVFTDPKQIFKSPGFPNEYEDNQICYWHIRLKYGQRIHLSFLDFDLEDDPGCLADYVEIYDSYDDVHGFVGRYCGDELPDDIISTGNVMTLKFLSDASVTAGGFQIKYVAMDPVSKSSQGKNTSTTSTGNKNFLAGRFSHL


We can provide the FITC labeling service, but the it is randomly labeled in vitro, the label is added at the free amino terminal of all lysine (primaquine),
it is not limited to the N-terminal of the protein, all the lysine residues will all be labeled. So this means more than one label is added.
There will be extra charges for labelling, lead time will be extended for 2-3 working days.
In addition, if you have some special demand for the tag, pls also keep us informed.
Q:

We are going to apply the protein to the in vitro cell and bind it to the receptor. Is it possible to guarantee?
At FITC labeling, you mentioned that there will be more than one labeling. Does it affect the protein structure?

A:
Thanks for your email. Sorry, we haven't validated its binding activity with the receptor, so it's not 100% guaranteed.
However, as what we provide is the full length of the mature protein and it's purified under very mild condition, in theory, it should be active.
In theory, the FITC labelling doesn't have much influence on the protein structure, but as we don't provide structure validation for all proteins,
we also haven't done FITC labelling for this protein before, so we can't 100% guarantee its structure will be correct. Probably the customer can purchase a small size to have a try.

靶点详情

  • 功能：
    Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.
  • 基因功能参考文献：
    1. TSG-6 up-regulation counteracts the progression of atherosclerosis. (Review) PMID: 29401724
    2. TSG-6 from MSCs attenuated severe burn-induced excessive inflammation via inhibiting activation of P38 and JNK signaling. PMID: 27444207
    3. TSG-6 secreted by human mesenchymal stem cells suppresses inflammatory reactions through p38 and ERK in the mitogen-activated protein kinase pathway PMID: 28247086
    4. This review will focus on the potential of mesenchymal stem/stromal cells for treatment of fibrotic diseases, with emphasis on the role of TSG-6 as a mediator of anti-inflammatory effects PMID: 26807758
    5. abnormally elevated in both the plasma and aortic wall of patients with abdominal aortic aneurysms PMID: 29180197
    6. Single nucleotide polymorphism in TNFAIP6 gene is associated with Systemic lupus erythematosus. PMID: 28246883
    7. TNFAIP6 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    8. Data show that TNF-stimulated gene-6 (TSG-6) interacts with chemokines through their glycosaminoglycans (GAGs)-binding sites and inhibits their binding to GAGs and endothelial cell surfaces. PMID: 27044744
    9. Data postulate that the molecular cross-linking enhanced by the multiple binding modes of the Link module might be critical for remodeling the ECM during inflammation/ovulation and might contribute to other functions of TSG-6. PMID: 26685054
    10. the well characterized hyaluronan-binding site in the TSG-6 Link module is not used for recognition during transfer of HCs onto HA. PMID: 26468290
    11. TSG-6 exhibited anti-inflammatory effects during the wound healing process and cicatrization and significantly diminished hypertrophic scar formation in a rabbit ear model PMID: 25661977
    12. results suggest that systemic administration of hASC or TSG-6 may be novel approaches to reverse CS-induced myelosuppression. PMID: 25329668
    13. TSG-6 activity in tissues, was examined. PMID: 25325979
    14. TSG-6 expression by hMSCs was induced 12 h after oropharyngeal delivery to LPS-exposed lungs. Knockdown of TSG-6 expression in hMSCs by RNA interference abrogated most of their anti-inflammatory effects. PMID: 21569482
    15. Heavy chain transfer by tumor necrosis factor-stimulated gene 6 to the bikunin proteoglycan. PMID: 25561734
    16. data suggest that TSG-6 expression might be essential in endometrial matrix organization and feto-maternal communication during the implantation process. PMID: 24213015
    17. the HA-binding site defined here may not play a role in TSG-6-mediated transfer of heavy chains from inter-alpha-inhibitor onto HA, a process known to be essential for ovulation. PMID: 24403066
    18. TSG-6 suppresses CXCL8-mediated chemotaxis of neutrophils; this lower potency effect might be important at sites where there is high local expression of TSG-6. PMID: 24501198
    19. This study provides insight into what we believe to be a previously undescribed multifaceted role of mesenchymal stem cell-released TSG-6 in wound healing. PMID: 23921952
    20. Data indicate that TSG-6-mediated cross-linking of hyaluronan (HA) films is impaired in the presence of inter-alpha-inhibitor (IalphaI) and that this effect suppresses the TSG-6-mediated enhancement of HA binding to CD44-positive cells. PMID: 24005673
    21. The protein product of tumor necrosis factor-alpha stimulated gene-6 strongly correlated with ossification markers and hyaluronidase in calcified human aortic valves. PMID: 23017666
    22. This review discusses TSG-6 as a potential molecular marker of oocyte maturation. PMID: 22922154
    23. TSG-6 increases the accumulation of HA in the cell-associated matrix, partially by preventing its dissolution from the cell-associated matrix into the conditioned medium, but primarily by inducing HA synthesis PMID: 23129777
    24. analysis of irreversible heavy chain transfer to hyaluronan oligosaccharides by tumor necrosis factor-stimulated gene-6 PMID: 23166324
    25. TSG-6 reverts the inhibitory effects exerted by PTX3 on FGF2-mediated angiogenesis through competition of FGF2/PTX3 interaction. PMID: 22267482
    26. TSG-6 was central to EMT through effects on HA macromolecular structure and through CD44-dependent triggering of cell responses. PMID: 21864707
    27. TSG-6 is a potent HA cross-linking agent PMID: 21596748
    28. TSG-6 protein, a negative regulator of inflammatory arthritis, forms a ternary complex with murine mast cell tryptases and heparin. PMID: 21566135
    29. negative correlation between TSG-6 expression levels and severity of capsular contracture (CC) suggests a possible protective role for TSG-6 in the context of CC formation, and this may have a clinically relevant role in prevention of breast CC PMID: 21239672
    30. TSG-6/HC2 link heavy chains randomly on the chondroitin sulfate chain of bikunin, in contrast to the ordered attachment observed during the biosynthesis. PMID: 20463016
    31. polymorphism in osteoarthritis; chromosome location given here (chromosome 2) PMID: 11854277
    32. TSG-6 is a multifunctional protein associated with inflammation [review] PMID: 12692188
    33. Tumor necrosis factor, alpha-induced protein 6 activates an anti-inflammatory agent- inter-alpha-inhibitor[review]. PMID: 14515153
    34. The TSG-6 and I alpha I interaction promotes a transesterification cleaving the protein-glycosaminoglycan-protein (PGP) cross-link. PMID: 15653696
    35. This TSG-6 model reveals the residues most likely to be involved in stabilizing the inter-Link module interface, which are highly conserved across the Link protein/ chondroitin sulfate proteoglycan subfamily. PMID: 15718240
    36. TSG-6 treatment also resulted in a rapid increase in cyclooxygenase-2 (COX-2) mRNA levels, suggesting that TSG-6 up-regulates COX-2 gene expression PMID: 15809059
    37. TSG-6 acts as cofactor and catalyst in the production of IalphaI heavy chain x hyaluronan complexes PMID: 15840581
    38. heparin with the Link module significantly increases the anti-plasmin activity of the TSG-6.IalphaI complex; TSG-6 is likely to contribute to matrix remodeling, at least in part, through down-regulation of the protease network PMID: 15917224
    39. The tumor necrosis factor alpha induced protein 6 is a potential marker that could be used for early diagnosis and prognosis of cancerous or precancerous lesions. PMID: 16467113
    40. hyaluronan synthase 2 and TSG-6 have roles in hyaluronan distribution and function in proximal tubular epithelial cells PMID: 16687630
    41. Evidence is cited in this review that TSG-6 can regulate the expression of various molecules that have important roles in the control of inflammation. PMID: 16709183
    42. TSG-6 is down-regulated during osteoblastic differentiation, and suppressed osteoblastic differentiation induced by both BMP-2 and osteogenic differentiation medium. PMID: 16771708
    43. TSG-6 may play an important protective role in bronchial epithelium by increasing the antiprotease screen on the airway lumen. PMID: 16873769
    44. Crystallography data on the free protein, and (15)N NMR relaxation data for the uncomplexed and HA(8)-bound forms of Link_TSG6, is presented. PMID: 17585936
    45. TSG-6 is a high affinity ligand that can mediate fibronectin interactions with other matrix components and modulate some interactions of fibronectin with cells PMID: 18042364
    46. 3 genes were upregulated in patients with chronic EBV infection: guanylate binding protein 1, tumor necrosis factor-induced protein 6, and guanylate binding protein 5; they may be associated with the inflammatory reaction or with cell proliferation. PMID: 18260761
    47. The transfer of heavy chains from bikunin proteins to hyaluronan requires both TSG-6 and HC2. PMID: 18448433
    48. TSG-6 has dual roles in bone remodeling: it inhibits RANKL-induced bone erosion in inflammatory diseases, and its interactions with BMP-2 and RANKL help to balance mineralization by osteoblasts and bone resorption by osteoclasts PMID: 18586671
    49. TSG-6 transfers proteins between glycosaminoglycans via a Ser28-mediated covalent catalytic mechanism PMID: 18820257
    50. myelomonocytic cells and MoDC are a major source of TSG-6 and that PTX3 and TSG-6 are coregulated PMID: 19389798

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  • 组织特异性：
    Found in the synovial fluid of patients with rheumatoid arthritis.
  • 数据库链接：

    HGNC: 11898

    OMIM: 600410

    KEGG: hsa:7130

    STRING: 9606.ENSP00000243347

    UniGene: Hs.437322