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  4. Recombinant Human Transcription factor E2F2 (E2F2)

Recombinant Human Transcription factor E2F2 (E2F2)

  • 货号：
    CSB-YP623909HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP623909HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP623909HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP623909HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP623909HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    dE2F2; E2F transcription factor 2; E2F-2; E2F2; E2F2_HUMAN; Transcription factor E2F2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-437
  • 氨基酸序列
    MLQGPRALAS AAGQTPKVVP AMSPTELWPS GLSSPQLCPA TATYYTPLYP QTAPPAAAPG TCLDATPHGP EGQVVRCLPA GRLPAKRKLD LEGIGRPVVP EFPTPKGKCI RVDGLPSPKT PKSPGEKTRY DTSLGLLTKK FIYLLSESED GVLDLNWAAE VLDVQKRRIY DITNVLEGIQ LIRKKAKNNI QWVGRGMFED PTRPGKQQQL GQELKELMNT EQALDQLIQS CSLSFKHLTE DKANKRLAYV TYQDIRAVGN FKEQTVIAVK APPQTRLEVP DRTEDNLQIY LKSTQGPIEV YLCPEEVQEP DSPSEEPLPS TSTLCPSPDS AQPSSSTDPS IMEPTASSVP APAPTPQQAP PPPSLVPLEA TDSLLELPHP LLQQTEDQFL SPTLACSSPL ISFSPSLDQD DYLWGLEAGE GISDLFDSYD LGDLLIN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.
  • 基因功能参考文献：
    1. miR-214 overexpression inhibits glioma cell growth in vitro and in vivo by inducing cell cycle arrest in G0/G1. Collectively, these data uncover a novel role for a PPARalpha-miR-214-E2F2 pathway in controlling glioma cell proliferation. PMID: 29862267
    2. miR-99a reveals two novel targets E2F2 and EMR2 that play a key role in lung tumourigenesis. By inhibiting E2F2 and EMR2, miR-99a represses in vivo the transition of epithelial cells through an EMT process concomitantly with the inhibition of stemness features and consequently decreasing the CSC population. PMID: 29072692
    3. Low E2F2 expression is associated with invasion in clear cell renal cell carcinoma. PMID: 26967247
    4. Data indicate E2F transcription factor 2 protein (E2F2) as the direct target of miR-31 in gastric cancer cells. PMID: 27174918
    5. These results suggest that miR-125a acts as a tumor suppressor via regulation of E2F2 expression in osteosarcoma progression, and miR-125a may represent a novel therapeutic target for the treatment of osteosarcoma. PMID: 28950256
    6. Accordingly, our results demonstrated that NAMPT is a prognostic marker in melanoma, and the identificationofNAMPT-E2F2-SIRT1 pathway establishes another link between NAMPT and apoptosis events in melanoma, with therapeutic implications for this deadly cancer. PMID: 28919418
    7. Compared with patients with variant genotypes of E2F2-rs2742976 and E2F2-rs3218123, patients with common homozygous genotypes had better disease-free survival (both log-rank, P < 0.001) and lower squamous cell carcinoma of the oropharynx recurrence risk (HR, 0.4, 95% CI, 0.3-0.6 and HR, 0.3, 95% CI, 0.2-0.5, respectively) after multivariable adjustment. PMID: 27864908
    8. Let-7b inhibits the malignant behavior of glioma cells and glioma stem-like cells via downregulation of E2F2. PMID: 27520092
    9. In NSCLC patients, E2F2 expression was found to be significantly correlated with sex and tumor size. E2F1 and E2F2 overexpression showed a significant association with poor prognosis. PMID: 27655285
    10. Data indicate that microRNA miR-214 has tumor-suppressive activity in hepatocellular carcinoma (HCC) through inhibition of E2F2 transcription factor (E2F2), cyclin-dependent kinases CDK3 and CDK6. PMID: 26498144
    11. we validated for the first time that E2F2 acts as a tumor activator in non-small cell lung carcinoma. PMID: 26617764
    12. E2F2 loss results in increased lung metastasis in breast cancer, potentially functioning through a PTPRD dependent mechanism. PMID: 26474282
    13. Elevated E2F2 expression in glioblastoma cells weakens the effect of elevated levels of miR-218. PMID: 26012781
    14. miR218 and miR520a are crucial in the development of hepatocellular carcinoma via the inhibition of cell proliferation and cycle progression by downregulating E2F2. PMID: 25816091
    15. miR-31 regulated the proliferation of colon cancer cells by targeting E2F2. PMID: 25362258
    16. Results show evidence that let-7a expression in osteosarcoma (OS) cells is significantly reduced and inversely correlated with E2F2 expression levels and that let-7a plays an important role in OS cell proliferation and tumorigenesis by targeting E2F2. PMID: 25647078
    17. Overall, miR-155 plays an important role in colorectal carcinoma tumorigenesis by negative regulation of its targets including E2F2. PMID: 24793496
    18. Low expression of E2F2 may reflect functional redundancy between members of the E2F family, in this case between E2F1 and E2F2. PMID: 22688350
    19. these results indicate that LXR ligands target gene networks, including those regulated by E2F family members, are critical for tumor biology and disease progression and merit further consideration as potential agents PMID: 23809258
    20. E2F2 knockdown can inhibit hESC proliferation and tumorigenicity without significantly harming stemness. PMID: 24446828
    21. Data suggest that the uniquely increased expression of CD38 and E2F2 in rheumatoid arthritis (RA) synovial tissues contribute to the immunoactivation of the disease. PMID: 24397353
    22. ALY is a novel E2F2-interacting protein and a relevant modulator of E2F-responsive gene expression. PMID: 23297349
    23. miR-125b plays important roles in regulating the proliferation of cancer stem cells by directly targeting E2F2. PMID: 22999819
    24. Overexpression of E2F transcription factor 2 is associated with nonepithelial ovarian cancer. PMID: 22531302
    25. Findings suggest that the deregulated activity of E2F in cancer cells causes increased activation of the Kpnbeta1 and Kpnalpha2 promoters, leading to elevated levels of these proteins, and ultimately impacting the cancer phenotype. PMID: 22125623
    26. the 3'UTR of E2F2 and CCND2 were directly bound to let-7a and let-7a down-regulated the expression of E2F2 and CCND2, suppressing prostate cancer cell proliferation in culture PMID: 20418948
    27. determination of interaction with RYBP and YY1 PMID: 12411495
    28. E2F2 has a role in blood pressure regulation via a component of the endothelin system PMID: 12566389
    29. Proliferation-promoting E2F transcription factor E2F-2 plays a pivotal role in the tumor biology of ovarian cancer. PMID: 17341621
    30. Up-regulation of E2F2 was detected in a set of 54 astrocytomas of different grades and significantly associated with malignancy. PMID: 17588684
    31. identify a novel E2F-binding site in the survivin promoter and show that mutation of either the p53- or E2F-binding sites is sufficient to increase promoter activity PMID: 17916908
    32. Decreased expression of E2F2 is associated with Increased expression of HER2 in breast tumors. PMID: 19142864
    33. miR-24 Inhibits cell proliferation by targeting E2F2, MYC, and other cell-cycle genes via binding to "seedless" 3'UTR microRNA recognition elements. PMID: 19748357

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    E2F/DP family
  • 组织特异性：
    Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.
  • 数据库链接：

    HGNC: 3114

    OMIM: 600426

    KEGG: hsa:1870

    STRING: 9606.ENSP00000355249

    UniGene: Hs.194333