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  4. Recombinant Human Cytotoxic T-lymphocyte protein 4 (CTLA4), partial (Active)

Recombinant Human Cytotoxic T-lymphocyte protein 4 (CTLA4), partial (Active)

In Stock
  • 货号：
    CSB-MP006163HU1
  • 规格：
    ￥1368
  • 图片：

    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized CD152 at 2 μg/ml can bind Anti-CD152 rabbit monoclonal antibody(CSB-RA213310A0HU), the EC50 of human CD152 protein is 27.14-34.82 ng/ml. Biological Activity Assay

    • The purity of CTLA4 was greater than 90% as determined by SEC-HPLC.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
    Greater than 90% as determined by SEC-HPLC.
  • 内毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    Measured by its binding ability in a functional ELISA. Immobilized CD152 at 2 μg/ml can bind Anti-CD152 rabbit monoclonal antibody(CSB-RA213310A0HU), the EC50 of human CD152 protein is 27.14-34.82 ng/ml.
  • 基因名：
  • Uniprot No.：
  • 别名：
    CTLA4; CD152; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD antigen CD152
  • 分子结构：
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    Mammalian cell
  • 分子量：
    42.5 kDa
  • 表达区域：
    37-162aa
  • 氨基酸序列
    AMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSDF
  • 蛋白标签：
    C-terminal hFc-tagged
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    Human CTLA4 (CD152) amino acid Ala37-Phe162 with an N-terminal linker, a TEV+linker, and a C-terminal human IgG1 Fc tag (Pro100-Lys330), was expressed in mammalian cells. The product is the recombinant human CTLA4 protein. Its bioactivity was measured in a functional ELISA. When the recombinant human CTLA4 is immobilized at 2 μg/ml, the anti-CTLA4 rabbit monoclonal antibody can bind to it with an EC50 of 27.14-34.82 ng/ml. It reaches up to 90% in purity determined by SDS-PAGE. On the gel, this CTLA4 protein ran to the molecular weight band of approximately 45 kDa. Its endotoxin content is less than 1.0 EU/ug determined by the LAL method. aND IT IS available now.

    CTLA4 is an inhibitory receptor inducibly expressed following T cell activation. CTLA4 either binds to CD80 or CD86, leading to a costimulatory or a co-inhibitory response, respectively. Due to the dampening effect of CTLA4, it plays a crucial role in the regulation of T-cell homeostasis and self-tolerance.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

平均分:
5.0分 - 1 个评价

实验应用：结合实验/蛋白间互作

产品评价： 我使用CSB-MP006163HU1产品进行抗体筛选，筛选的阳性细胞株特异性好，灵敏度高，空白对照背景低，实验很顺利，极大地节省了时间。

By 李老师

靶点详情

  • 功能：
    Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
  • 基因功能参考文献：
    1. PTPN22 and CTLA-4 polymorphisms are associated with Autoimmune polyglandular syndromes and differentiate between polyglandular and monoglandular autoimmunity. PMID: 29409002
    2. CTLA4 expression levels was found significantly lower in the alopecia areata patients in Iranian cohort; no association between CTLA4 genetic polymorphism and susceptibility to alopecia areata PMID: 29979892
    3. The CTLA-4 gene +49 A/G polymorphism and the NOD2/CARD15 gene N852S polymorphism were not associated with CD or UC in a Turkish population PMID: 30213296
    4. The CTLA4 gene is suggested to correlate with immune thrombocytopenia through its abnormal expression level instead of gene site mutation. PMID: 30319055
    5. Paget disease is characterized by an intense lymphocytic response, devoid of the immune-suppressive impact of the PD-L1 pathway, but with occasional CTLA-4 expression PMID: 29943071
    6. Depending on the environmental conditions, Mesenchymal stem/stromal cells express different isoforms of CTLA-4 with the secreted isoform (sCTLA-4) being the most abundant under hypoxic conditions. Furthermore, the immunosuppressive function of Mesenchymal stem/stromal cells is mediated mainly by the secretion of CTLA-4. PMID: 30087255
    7. Increased frequency and CTLA-4-expression of Varicella Zoster Virus-specific T cells from cerebrospinal fluid or blood are specifically found in patients with Varicella Zoster Virus-related Central Nervous System-infection. PMID: 28845512
    8. Rs56102377 in the 3'-UTR of CTLA4 may act as a protective factor by disrupting the regulatory role of miR-105 in CTLA4 expression. PMID: 30355938
    9. The present work showed in West Algerian population that the HLA-B27 antigen and the variation in the CTLA4 3'UTR region played an important role in the ankylosing spondylitis susceptibility. The heterogeneity of this disease is deduced by genetic difference found between B27+ and B27- groups. PMID: 29675891
    10. High CTLA4 expression is associated with Melanoma. PMID: 29150430
    11. CTLA4 protein had significantly higher serum level in recurrent spontaneous abortion patients than in healthy controls. In recurrent spontaneous abortion patients, AA genotype carriers had higher CTLA4 serum level than that GG genotype carriers. Minor alleles of CTLA4 polymorphisms might inhibit the recurrent spontaneous abortion susceptibility via upregulated the protein expression level. PMID: 30334961
    12. The results in our meta-analysis indicated that CTLA4 +49A/GG allele/AA genotype was associated with the risk of colorectal cancer in the Asian population and overall populations PMID: 29970719
    13. The CTLA4 -318/C/T SNP was associated with an increased risk to develop IgAN, while the CT60 G/A genotype significantly associated with the risk for higher proteinuria PMID: 29539619
    14. This review summarizes the current literature relevant to T cell exhaustion in patients with Hepatitis B virus (HBV)related chronic hepatitis, and discusses the roles of CTLA4 in T cell exhaustion. [review] PMID: 29786112
    15. Study provides evidence that CTLA4 +49 A/G (Thr/Ala) polymorphism was strongly associated with T1diabetes in south India. PMID: 29603038
    16. gene polymorphism is associated with psoriasis in Turkish population PMID: 29850619
    17. The mRNA expression of FAS was lower in patients with TP53 mutation than TP53 wild-type. Our findings suggest that TP53 mutation is a potential negative predictor of metastatic melanoma treated with CTLA-4 blockade. PMID: 29793878
    18. TSA results indicated that CTLA-4 +49A/G should be considered as a biomarker for HT, whereas both the CT60 and -318C/T SNPs warrant confirmation by further studies PMID: 29461867
    19. susceptibility to RSA was subject to the synthetic regulation of chromosomal aberrations and genetic mutations within CLTA-4 and Foxp3, suggesting that the conduction of karyotype analysis and genetic detection for RSA patients could effectively guide effective RSA counseling and sound child rearing. PMID: 29476189
    20. CTLA4 missense variant significantly associates with inhibitor development in Argentine patients with severe haemophilia A PMID: 28220572
    21. Study suggests that miR-487a-3p might repress CTLA4 and FOXO3 by binding to their 3'UTRs and contribute to the development of T1D. PMID: 29859273
    22. the expression of mCTLA-4 in skin lesion inversely correlated with the severity of psoriasis and CTLA-4 might play a critical role in the disease severity of psoriasis. PMID: 29305257
    23. Hematopoietic stem cell transplantation for CTLA4 deficiency with pathogenic mutations resulting in complex immune dysregulation syndromes. PMID: 27102614
    24. Our results suggest that CTLA-4 may be involved in lipid metabolism and affect Type 2 diabetes mellitus (T2DM)disease progression and/or the development of diabetic complications although this gene does not represent a major risk factor for T2DM. PMID: 29511375
    25. rs231775, rs4553808 and rs5742909 but not rs3087243 and rs733618 were significantly related to cancer risk. In analyses stratified by ethnicity, both rs231775 and rs4553808 were significant susceptibility polymorphisms in an Asian population but not in a Caucasian population. PMID: 29794444
    26. Paper analyses results of serum cytokines and lymphocyte apoptosis study in nodular goiter against the background of autoimmune thyroiditis and thyroid adenoma based on the cell preparedness to apoptosis, the number of apoptotic lymphocytes and the content of proapoptotic tumor necrosis factor-alpha, interleukins in serum, considering the polymorphism of BCL-2, CTLA-4 and APO-1 genes. PMID: 29250672
    27. -318C/T polymorphism of CTLA-4 gene might play a significant role in the development of SLE in the Iranian patients. PMID: 24400885
    28. the immune response to specific miHA mismatches is modulated by the CTLA-4 genotype of the donor PMID: 28827064
    29. It was concluded that the abnormal expression of endometrial E2A existed in mid-secretory endometrium of women with recurrent miscarriage, and there was a positive correlation between E2A and FOXP3, and E2A and CTLA-4, suggesting the possible regulatory role of E2A in endometrium receptivity. PMID: 29270752
    30. This study shows a significant overexpression of CTLA-4 in >50% of breast carcinomas with no such overexpression of CTLA-4 in benign breast tissues. PDL-1 staining is seen in only a small number of invasive ductal carcinomas (4.1%). PMID: 29672601
    31. CTLA4 gene is suggested to correlated with polycyctic ovary syndrome, and influence polycycstic ovary syndrome through regulating obesity and the homeostatic model assessment for insulin resistance in a novel way. PMID: 30024513
    32. We describe three cases of patients with mRCC treated with anti-PD-1 antibody therapy in combination with targeted therapy (bevacizumab), anti-cytotoxic T lymphocyte antigen 4 therapy (ipilimumab), or radiotherapy. PMID: 29146617
    33. The CTLA-4c.49A>G and CTLA-4g.319C>T single nucleotide polymorphisms might be considered as low risk susceptibility locus for prostate cancer PMID: 28101800
    34. anti-CTLA4/anti-PD-1/PD-L1 combinations versus anti-PD-1/PD-L1 monotherapy was selected as a factor independent of TMB for predicting better RR (77% vs. 21%; P = 0.004) and PFS (P = 0.024). Higher TMB predicts favorable outcome to PD-1/PD-L1 blockade across diverse tumors. PMID: 28835386
    35. Polymorphisms at IL10 (-1082 G>A), IL4 (-589 C>T), CTLA4 (+49A>G), and DAO (+8956 C>G) genes were studied in 55 cases. PMID: 28750137
    36. this study showed that CTLA-4 + 49A/G polymorphism was not correlated with greater genetic risk for leprosy. However, GG genotype was associated with older age, older age of onset and over-representation in male in an Iranian Azeri population. PMID: 29104093
    37. Our meta-analysis suggested that the +49 A/G polymorphism in CTLA4 might be a risk factor for asthma susceptibility, especially in Asian individuals, children, and patients with atopy PMID: 29995780
    38. Genetic polymorphisms of CTLA-4 gene on the nucleotide 49 at codon 17 of exon 1, TSHR gene SNP rs2268458 of intron 1, number of regulatory T cells and TRAb levels play a role as risk factors for relapse in patients with Graves' disease. PMID: 29093229
    39. These results demonstrate that POSTN promotes the osteogenic differentiation of mesenchymal stem cells (MSCs) and that CTLA4 enhances the ectopic osteogenesis of MSCs-CTLA4-based tissue-engineered bone. PMID: 28687929
    40. the polymorphism -318C/T of CTLA-4 gene is associated with RBC alloimmunization among sickle cell disease patients. This highlights the role played by CTLA-4 on post-transfusion alloantibody development PMID: 28815969
    41. Meta-analysis found that CTLA4 -318C/T gene polymorphism is not associated with the risk of acute rejection in renal transplantation in overall populations. PMID: 28449371
    42. Our goal was to stimulate antitumor immunity by combining SS1P or LMB-100 with anti-CTLA-4. We constructed a BALB/c breast cancer cell line expressing human mesothelin (66C14-M), which was implanted in one or two locations. SS1P or LMB-100 was injected directly into established tumors and anti-CTLA-4 administered i.p. In mice with two tumors, one tumor was injected with immunotoxin and the other was not. PMID: 28674083
    43. Taken together, we found that Id3+ and CTLA-4+ endometrial cells were significantly higher in women with repeated implantation failure and recurrent miscarriage, suggesting the negative roles of these angiogenesis and immune tolerance markers involving in regulating endometrium receptivity. PMID: 28224680
    44. study indicated that the polymorphisms of rs231775 and rs231725 would be the risk factors of Primary Biliary Cholangitis [meta-analysis] PMID: 28642883
    45. Suggest that genetic polymorphisms of CTLA-4 function as sex-dependent risk factors for development of acute rejection in an Iranian kidney transplant population. PMID: 28031007
    46. A phase Ib study of dasatinib plus ipilimumab in patients with gastrointestinal stromal tumor (GIST) and other sarcomas was performed on the basis of preclinical data demonstrating that combined KIT and CTLA-4 blockade is synergistic. PMID: 28007774
    47. The data we presented here showed that CTLA-4 was highly expressed in regulatory T cells and PD-1 decreased in CD8+ T cells in peripheral blood of SCLC patients, suggesting their unique mechanisms involved in immune regulation. PMID: 29167005
    48. Significant differences in the CpG-methylation patterns between tumor tissues and matched controls were observed for CTLA4 showing a decreased methylation of this gene in non-small cell lung cancer patients. Expression studies confirmed that hypomethylation also resulted in increased expression of CTLA4. PMID: 28503213
    49. The polymorphisms +49 G/A, -1661 A/G and -318 C/T may elevate the susceptibility to BC, but the polymorphism CT60 G/A may offer protection against the cancer. PMID: 28416762
    50. In children with idiopathic nephrotic syndrome (INS), serum CTLA-4 concentration significantly increased at remission compared with onset. Furthermore, a positive significant correlation was observed between Treg number and serum CTLA-4 level. This suggests that Treg and CTLA-4 are involved in the induction of remission in INS. PMID: 28544686

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  • 相关疾病：
    Systemic lupus erythematosus (SLE); Diabetes mellitus, insulin-dependent, 12 (IDDM12); Celiac disease 3 (CELIAC3); Autoimmune lymphoproliferative syndrome 5 (ALPS5)
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalization.
  • 组织特异性：
    Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation.
  • 数据库链接：

    HGNC: 2505

    OMIM: 109100

    KEGG: hsa:1493

    STRING: 9606.ENSP00000303939

    UniGene: Hs.247824