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SNAP23 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA827145A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA827145A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    SNAP23,  a soluble N-ethyl-maleimide sensitive fusion protein attachment protein receptor (SNARE) molecule, is essential for secretory granule fusion in several cell lines. SNAP23 is involved in exocytotic events in diverse nonneuronal cells, such as surfactant release from alveolar epithelial cells, glucose transporter GLUT4 translocation in adipocytes, and Ig release from plasma cells. In pancreatic β cells, SNAP23 also promotes the fusion of insulin granules to the plasma membrane, whereas in pancreatic acinar cells, SNAP23 binds VAMP2 or VAMP8 for the fusion of amylase granules to the plasma membrane.

    The production of this recombinant SNAP23 antibody started with identifying and cloning the genes for antibody expression. After the SNAP23 antibody was cloned into an expression plasmid, the plasmid could be introduced into the mammalian cell to produce the target recombinant antibody. This recombinant SNAP23 antibody has been validated in ELISA, IHC.

  • Uniprot No.：
    O00161
  • 基因名：
    SNAP23
  • 别名：
    Synaptosomal-associated protein 23 (SNAP-23) (Vesicle-membrane fusion protein SNAP-23), SNAP23
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human SNAP23
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    2D8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
  • 基因功能参考文献：
    1. PKM2 promotes tumor cell exosome release via phosphorylating protein SNAP23. PMID: 28067230
    2. SNAP23 suppressed progression of cervical cancer and induced cell cycle G2/M arrest via upregulating p21(cip1) and downregulating CyclinB1 PMID: 29908998
    3. Knockdown of VAMP3 and SNAP23 reduces endothelial secretion of miR-126-3p and miR-200a-3p, as well as the proliferation, migration, and suppression of contractile markers in smooth muscle cells caused by vascular endothelial cell-coculture. PMID: 28716920
    4. Study showed that SNAP23 was recruited to the close sites of lipid droplet (LD) in HCV-infected cells, implying that SNAP23 was required for HCV-induced LD enlargement and, HCV production. PMID: 27550144
    5. A novel regulatory mechanism for SNAP23-dependent mast cell activation of T. vaginalis-secreted LTB4 involving surface trafficking of BLT1. PMID: 27795355
    6. Study identified SNAP23 as a novel oncogene in Ovarian Cancer (OC). It is over-expressed in OC and could promote the proliferation, migration and invasion of OC in vitro. PMID: 27855700
    7. Localization of SNAP23 was found in plasma membrane, lipid droplets and mitochondria of skeletal muscle. PMID: 26733245
    8. these data suggest that SNAP23 is a key component of the endothelial SNARE machinery that mediates endothelial exocytosis. PMID: 26266817
    9. Increased level of SNAP23-Syntaxin4-VAMP7 interaction correlates with decreased Syntaxin4 phosphorylation and trafficking of MT1-MMP to invadopodia during cellular invasion. PMID: 24807903
    10. The association of Src, EGFR and beta1 integrin is dependent upon membrane traffic that is mediated by syntaxin13 (officially known as STX12) and SNAP23. PMID: 24496451
    11. Data suggest SNAP23 and VAMP3 (vesicle-associated membrane protein 3) participate in interleukin-1beta-, interleukin-1 receptor-, calcium signaling-dependent secretion/exocytosis of interleukin-6 and tumor necrosis factor alpha from synoviocytes. PMID: 24373201
    12. Results suggest that phagosomal SNAP-23 is one of the key players regulating the phagosomal environment in macrophages. PMID: 23087210
    13. STX-3 and SNAP-23 are crucial for the release of all chemokines in mature human mast cells PMID: 21981832
    14. Data show that knockdown of SNAP-23 inhibited the production of virus. PMID: 21768361
    15. Introduction of the SNARE domain of SNAP-23 into neutrophils as an HIV transactivator of transcription (TAT) fusion protein significantly inhibits exocytosis of neutrophil granule subsets without altering signal transduction pathway activation. PMID: 21642540
    16. SNAP23 and SNAP25 palmitoylation is regulated by DHHC palmitoyl transferases PMID: 20519516
    17. the importance of SNAP23 in the trafficking of matrix metalloproteinases during degradation of extracellular matrix substrates and subsequent cellular invasion PMID: 19910495
    18. SNAP-23 and syntaxin-4 are expressed in human eosinophils and are likely candidates for association with VAMP-2 during docking, which is followed by exocytosis. PMID: 11842301
    19. cleaved by calpain in activated platelets PMID: 12121992
    20. CFTR-mediated chloride currents are inhibited by introducing excess SNAP-23 into HT29-Cl.19A epithelial cells. PMID: 12209004
    21. A synaptosome-associated protein of 23 kDa(SNAP23), the ubiqitously expressed homologue of SNAP25, has been shown to interact directly with ubiquitous kinesin heavy chain (uKHC). PMID: 12475239
    22. Palmitoylated peptides from the cysteine-rich domain of this protein cause membrane fusion depending on peptide length, position of cysteines, and extent of palmitoylation. PMID: 12551899
    23. an examination of the homotetrameric structure of the N-terminal domain of this protein PMID: 12556468
    24. studies show that synaptosomal-associated protein-23 is phosphorylated in platelets during cell activation through a protein kinase C-related mechanism at two or more sites PMID: 12930825
    25. Because SNAP-23 plays a central role in SNAREs complex formation, it was chosen to examine possible functional implications of the SNARE system in plasma cell Ig secretion. PMID: 16272324
    26. expression of SNAP-23 and syntaxin-2 on the extracellular surface of the platelet plasma membrane. PMID: 17485553
    27. SNAP-23 is not essential for constitutive exocytosis of secreted alkaline phosphatase PMID: 17825825
    28. The expression of the SNARE protein SNAP-25 and its cellular homologue SNAP-23, as well as syntaxin1 and VAMP (vesicle-associated membrane protein) in samples of normal parathyroid tissue, chief cell adenoma, and parathyroid carcinoma, was examined. PMID: 18457912
    29. Overexpression of a dominant negative SNAP-23 mutant suppressed expression of P-selectin, CD40L, CD41, CD61, release from dense granules and platelet aggregation PMID: 18665925

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Note=Mainly localized to the plasma membrane.
  • 蛋白家族：
    SNAP-25 family
  • 组织特异性：
    Ubiquitous. Highest levels where found in placenta.
  • 数据库链接：

    HGNC: 11131

    OMIM: 602534

    KEGG: hsa:8773

    STRING: 9606.ENSP00000249647

    UniGene: Hs.511149