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LTA4H Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA198749A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, 293T whole cell lysate, PC-3 whole cell lysate, Mouse Brain whole cell lysate
      All lanes: LTA4H antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 70, 60, 58, 67 kDa
      Observed band size: 70 kDa
  • 其他：

产品详情

  • 产品描述：

    LTA4H, an enzyme that converts LTA4 to LTB4, regulates the balance between the anti-inflammatory lipoxins and pro-inflammatory LTB4, with direct implications for TB-driven inflammation. In addition to its epoxide hydrolase activity, LTA4H also possesses anion-dependent aminopeptidase activity. As an aminopeptidase, LTA4H degrades the N-terminus of peptides. The aminopeptidase activity of LTA4H is generally assumed to be involved in the processing of peptides related to inflammation and host defense and may be involved in human cancer. Overexpression of LTA4H has been found in several human cancers including skin cancer.

    The vectors expressing anti-LTA4H antibody were constructed as follows: immunizing an animal with A synthesized peptide derived from human LTA4H, isolating the positive splenocyte and extracting RNA, obtaining DNA by reverse transcription, sequencing and screening LTA4H antibody gene, and amplifying heavy and light chain sequence by PCR and cloning them into plasma vectors. After that, the vector clones were transfected into the mammalian cells for production. The product is the recombinant LTA4H antibody. Recombinant LTA4H antibody in the culture medium was purified using Affinity-chromatography. It can react with LTA4H protein from Human, Mouse and is used in the ELISA, WB.

  • Uniprot No.：
    P09960
  • 基因名：
    LTA4H
  • 别名：
    Leukotriene A-4 hydrolase (LTA-4 hydrolase) (EC 3.3.2.6) (Leukotriene A(4) hydrolase), LTA4H, LTA4
  • 反应种属：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from human LTA4H
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    6G7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the proinflammatory mediator leukotriene B4 (LTB4). Has also aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides. In addition to its proinflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL). Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions.
  • 基因功能参考文献：
    1. The structures reveal that a single catalytic water is involved in both catalytic activities of LTA4H, alternating between epoxide ring opening and peptide bond hydrolysis, assisted by E271 and E296, respectively. Moreover, we have found two conformations of LTA4H, uncovering significant domain movements. PMID: 28827365
    2. A higher incidence of severe IRIS among patients with mutant LTA4H genotypes (CT and TT) was observed compared to the wild type, despite similar IRIS incidence and immune restoration in both groups. Steroids were effective in alleviating IRIS in all the genotypes PMID: 27643598
    3. The LTA4H promoter polymorphism correlated with CSF mononuclear cell count but not with mortality in tuberculous meningitis PMID: 28419315
    4. LTA4H genotype predicted survival of HIV-uninfected patients, with TT-genotype patients significantly more likely to survive tuberculous meningitis than CC-genotype patients. LTA4H genotype and HIV infection influence pretreatment inflammatory phenotype and survival from tuberculous meningitis. LTA4H genotype may predict adjunctive corticosteroid responsiveness in HIV-uninfected individuals. PMID: 28419368
    5. LTA4H promoter region SNP was associated with susceptibility to bacteriologically confirmed bacterial meningitis but did not influence clinical presentation, disease severity or survival following dexamethasone treatment. PMID: 25799317
    6. Report no association between LTA4H SNPs and atherosclerotic plaque phenotypes. PMID: 25721704
    7. The best proteinogenic amino acid recognized by LTA4H is arginine. PMID: 24573245
    8. The results of our study failed to confirm whether the selected variants in LTA4H gene within the LT metabolism pathway contribute to platelet reactivity in a diabetic population treated with ASA. PMID: 23828562
    9. The LTA4H A-9188>G polymorphism has a strong association with severe asthma in children. PMID: 23573270
    10. Data indicate that 13S,14S-epoxy-docosahexaenoic acid (DHA) inhibits leukotriene B4 (LTB4) biosynthesis by leukotriene A4 hydrolase (LTA4H). PMID: 23504711
    11. We identified a novel leukotriene haplotype that appears to be protective toward subclinical atherosclerosis. This association is modified by dietary intake of polyunsaturated fatty acids PMID: 23153620
    12. genetic association studies in population of Han Chinese in Eastern China: Using a recessive genetic model, an SNP in LTA4H (rs6538697) is associated with increased risk of ischemic stroke. PMID: 23079278
    13. Structural origins for the loss of catalytic activities of bifunctional human LTA4H revealed through molecular dynamics simulations. PMID: 22848428
    14. It was found modest association with LTA4H rs1978331C (intron 11) with increased FEV1 (p = 0.029) and with increased FEV1/FVC ratio (p = 0.020). PMID: 22206291
    15. common polymorphisms in the LTA4H gene do not play any major role in susceptibility to clinical pulmonary tuberculosis PMID: 21112816
    16. LTA4H and ALOX5AP gene polymorphisms modify the augmentation of bronchodilator responsiveness by leukotriene modifiers in Puerto Ricans but not Mexicans with asthma. PMID: 20810156
    17. Our results support the role of LTA4H and ALOX5AP variants as risk factors for asthma in Latino populations. PMID: 20067482
    18. Several point mutants of LTA4H with altered substrate specificities were designed. PMID: 20432426
    19. In humans, protection from both tuberculosis and multibacillary leprosy is associated with heterozygosity for LTA4H polymorphisms that have previously been correlated with differential LTB(4) production. PMID: 20211140
    20. The 5-lipoxygenase-leukotriene A4 pathway might play roles in the proliferation of human glioma cells. PMID: 19919819
    21. 5-lipoxygenase and leukotriene A4 hydrolase expression in atherosclerotic lesions correlates with symptoms of plaque instability PMID: 16698924
    22. leukotriene A4 hydrolase These data provide evidence for the role of SNPs spanning the ALOX5AP and LTA4H genes in asthma and atopy susceptibility PMID: 18547289
    23. Analysis of the thermodynamic parameters of irreversible thermal unfolding suggests that entropy-driven factors are responsible for the fast unfolding rate of the cold-adapted aminopeptidase. PMID: 18599387
    24. Observational study and clinical trial of gene-environment interaction and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 15886380

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase M1 family
  • 组织特异性：
    Isoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts.
  • 数据库链接：

    HGNC: 6710

    OMIM: 151570

    KEGG: hsa:4048

    STRING: 9606.ENSP00000228740

    UniGene: Hs.524648