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  4. Recombinant Human Leukotriene A-4 hydrolase (LTA4H)

Recombinant Human Leukotriene A-4 hydrolase (LTA4H)

  • 中文名称：
    人LTA4H重组蛋白
  • 货号：
    CSB-YP013219HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人LTA4H重组蛋白
  • 货号：
    CSB-EP013219HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人LTA4H重组蛋白
  • 货号：
    CSB-EP013219HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人LTA4H重组蛋白
  • 货号：
    CSB-BP013219HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人LTA4H重组蛋白
  • 货号：
    CSB-MP013219HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    LTA4H
  • Uniprot No.：
  • 别名：
    FLJ17564; Leukotriene A(4) hydrolase; Leukotriene A-4 hydrolase; Leukotriene A4 hydrolase; LKHA4_HUMAN; LTA-4 hydrolase; LTA4; LTA4 hydrolase; LTA4H
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-611
  • 氨基酸序列
    PEIVDTCSL ASPASVCRTK HLHLRCSVDF TRRTLTGTAA LTVQSQEDNL RSLVLDTKDL TIEKVVINGQ EVKYALGERQ SYKGSPMEIS LPIALSKNQE IVIEISFETS PKSSALQWLT PEQTSGKEHP YLFSQCQAIH CRAILPCQDT PSVKLTYTAE VSVPKELVAL MSAIRDGETP DPEDPSRKIY KFIQKVPIPC YLIALVVGAL ESRQIGPRTL VWSEKEQVEK SAYEFSETES MLKIAEDLGG PYVWGQYDLL VLPPSFPYGG MENPCLTFVT PTLLAGDKSL SNVIAHEISH SWTGNLVTNK TWDHFWLNEG HTVYLERHIC GRLFGEKFRH FNALGGWGEL QNSVKTFGET HPFTKLVVDL TDIDPDVAYS SVPYEKGFAL LFYLEQLLGG PEIFLGFLKA YVEKFSYKSI TTDDWKDFLY SYFKDKVDVL NQVDWNAWLY SPGLPPIKPN YDMTLTNACI ALSQRWITAK EDDLNSFNAT DLKDLSSHQL NEFLAQTLQR APLPLGHIKR MQEVYNFNAI NNSEIRFRWL RLCIQSKWED AIPLALKMAT EQGRMKFTRP LFKDLAAFDK SHDQAVRTYQ EHKASMHPVT AMLVGKDLKV D
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the proinflammatory mediator leukotriene B4 (LTB4). Has also aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides. In addition to its proinflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL). Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions.
  • 基因功能参考文献：
    1. The structures reveal that a single catalytic water is involved in both catalytic activities of LTA4H, alternating between epoxide ring opening and peptide bond hydrolysis, assisted by E271 and E296, respectively. Moreover, we have found two conformations of LTA4H, uncovering significant domain movements. PMID: 28827365
    2. A higher incidence of severe IRIS among patients with mutant LTA4H genotypes (CT and TT) was observed compared to the wild type, despite similar IRIS incidence and immune restoration in both groups. Steroids were effective in alleviating IRIS in all the genotypes PMID: 27643598
    3. The LTA4H promoter polymorphism correlated with CSF mononuclear cell count but not with mortality in tuberculous meningitis PMID: 28419315
    4. LTA4H genotype predicted survival of HIV-uninfected patients, with TT-genotype patients significantly more likely to survive tuberculous meningitis than CC-genotype patients. LTA4H genotype and HIV infection influence pretreatment inflammatory phenotype and survival from tuberculous meningitis. LTA4H genotype may predict adjunctive corticosteroid responsiveness in HIV-uninfected individuals. PMID: 28419368
    5. LTA4H promoter region SNP was associated with susceptibility to bacteriologically confirmed bacterial meningitis but did not influence clinical presentation, disease severity or survival following dexamethasone treatment. PMID: 25799317
    6. Report no association between LTA4H SNPs and atherosclerotic plaque phenotypes. PMID: 25721704
    7. The best proteinogenic amino acid recognized by LTA4H is arginine. PMID: 24573245
    8. The results of our study failed to confirm whether the selected variants in LTA4H gene within the LT metabolism pathway contribute to platelet reactivity in a diabetic population treated with ASA. PMID: 23828562
    9. The LTA4H A-9188>G polymorphism has a strong association with severe asthma in children. PMID: 23573270
    10. Data indicate that 13S,14S-epoxy-docosahexaenoic acid (DHA) inhibits leukotriene B4 (LTB4) biosynthesis by leukotriene A4 hydrolase (LTA4H). PMID: 23504711
    11. We identified a novel leukotriene haplotype that appears to be protective toward subclinical atherosclerosis. This association is modified by dietary intake of polyunsaturated fatty acids PMID: 23153620
    12. genetic association studies in population of Han Chinese in Eastern China: Using a recessive genetic model, an SNP in LTA4H (rs6538697) is associated with increased risk of ischemic stroke. PMID: 23079278
    13. Structural origins for the loss of catalytic activities of bifunctional human LTA4H revealed through molecular dynamics simulations. PMID: 22848428
    14. It was found modest association with LTA4H rs1978331C (intron 11) with increased FEV1 (p = 0.029) and with increased FEV1/FVC ratio (p = 0.020). PMID: 22206291
    15. common polymorphisms in the LTA4H gene do not play any major role in susceptibility to clinical pulmonary tuberculosis PMID: 21112816
    16. LTA4H and ALOX5AP gene polymorphisms modify the augmentation of bronchodilator responsiveness by leukotriene modifiers in Puerto Ricans but not Mexicans with asthma. PMID: 20810156
    17. Our results support the role of LTA4H and ALOX5AP variants as risk factors for asthma in Latino populations. PMID: 20067482
    18. Several point mutants of LTA4H with altered substrate specificities were designed. PMID: 20432426
    19. In humans, protection from both tuberculosis and multibacillary leprosy is associated with heterozygosity for LTA4H polymorphisms that have previously been correlated with differential LTB(4) production. PMID: 20211140
    20. The 5-lipoxygenase-leukotriene A4 pathway might play roles in the proliferation of human glioma cells. PMID: 19919819
    21. 5-lipoxygenase and leukotriene A4 hydrolase expression in atherosclerotic lesions correlates with symptoms of plaque instability PMID: 16698924
    22. leukotriene A4 hydrolase These data provide evidence for the role of SNPs spanning the ALOX5AP and LTA4H genes in asthma and atopy susceptibility PMID: 18547289
    23. Analysis of the thermodynamic parameters of irreversible thermal unfolding suggests that entropy-driven factors are responsible for the fast unfolding rate of the cold-adapted aminopeptidase. PMID: 18599387
    24. Observational study and clinical trial of gene-environment interaction and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 15886380

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase M1 family
  • 组织特异性：
    Isoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts.
  • 数据库链接：

    HGNC: 6710

    OMIM: 151570

    KEGG: hsa:4048

    STRING: 9606.ENSP00000228740

    UniGene: Hs.524648