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Acetyl-Histone H2A type 1-B/E (K9) Recombinant Monoclonal Antibody

  • 货号:
    CSB-RA010385A09acHU
  • 规格:
    ¥1320
  • 图片:
    • Immunocytochemistry analysis of CSB-RA010385A09acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010385A09acHU at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他:

产品详情

  • Uniprot No.:
    P04908
  • 基因名:
    HIST1H2AB
  • 别名:
    H2a 615 antibody; H2A antibody; H2A GL101 antibody; H2A histone family member A antibody; H2A.1 antibody; H2A.2 antibody; H2A/a antibody; H2A/m antibody; H2A/O antibody; H2A/q antibody; H2A1B_HUMAN antibody; H2AFA antibody; H2AFE antibody; H2AFL antibody; H2AFM antibody; H2AFO antibody; H2AFQ antibody; HIST1H2AE antibody; HIST1H2AJ antibody; HIST2H2AA antibody; HIST2H2AA3 antibody; HIST2H2AB antibody; HIST2H2AC antibody; Histone 1 H2ae antibody; Histone 2 H2aa3 antibody; Histone 2 H2ab antibody; Histone 2 H2ac antibody; Histone H2A type 1 B antibody; Histone H2A type 1 C antibody; Histone H2A type 1 E antibody; Histone H2A type 1 J antibody; Histone H2A type 1-B/E antibody; Histone H2A.2 antibody; Histone H2A/a antibody; Histone H2A/m antibody; MGC74460 antibody
  • 反应种属:
    Human
  • 免疫原:
    A synthesized peptide
  • 免疫原种属:
    Homo sapiens (Human)
  • 标记方式:
    Non-conjugated
  • 克隆类型:
    Monoclonal
  • 抗体亚型:
    Rabbit IgG
  • 纯化方式:
    Affinity-chromatography
  • 克隆号:
    3H5
  • 浓度:
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液:
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式:
    Liquid
  • 应用范围:
    ELISA, ICC, IF
  • 推荐稀释比:
    Application Recommended Dilution
    ICC 1:50-1:500
    IF 1:30-1:200
  • Protocols:
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能:
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 亚细胞定位:
    Nucleus. Chromosome.
  • 蛋白家族:
    Histone H2A family
  • 数据库链接:

    HGNC: 4734

    OMIM: 602786

    KEGG: hsa:3012

    STRING: 9606.ENSP00000303373

    UniGene: Hs.121017