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  4. FIS1 Recombinant Monoclonal Antibody

FIS1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA334524A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, U251 whole cell lysate, A549 whole cell lysate
      All lanes: FIS1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 17 kDa
      Observed band size: 17-22 kDa
    • IHC image of CSB-RA334524A0HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA334524A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • Uniprot No.：
    Q9Y3D6
  • 基因名：
  • 别名：
    Mitochondrial fission 1 protein (FIS1 homolog) (hFis1) (Tetratricopeptide repeat protein 11) (TPR repeat protein 11), FIS1, TTC11
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human FIS1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    27B4
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.
  • 基因功能参考文献：
    1. Fis1 depletion in osteoarthritis impairs chondrocyte survival and peroxisomal and lysosomal function. PMID: 27497958
    2. Results show that Fis1 is significantly overexpressed in malignant oncocytomas. PMID: 25822260
    3. Study revealed that a novel mitochondrial fission pathway composed of miR-483-5p and FIS1 regulates cisplatin sensitivity. PMID: 25843291
    4. Overexpression of FoxM1 and Plk1 in Fis1-depleted cells restores mitotic entry. PMID: 23907611
    5. Fis1 expression is significantly increased in spinal cord in a transgenic mouse model of amyotrophic lateral sclerosis. PMID: 23713734
    6. Fis1 acts as a mitochondrial recruitment factor for TBC1D15 that is involved in regulation of mitochondrial morphology. PMID: 23077178
    7. PEX11 proteins attract both Mff and human Fis1 (hFis1) to their site of action. PMID: 22595523
    8. Fis1 and dynamin-related protein (Drp)1 levels are remarkably increased in peripheral blood lymphocytes of Alzheimer's disease patients. PMID: 22340708
    9. Fis1 activity depends on its ability to interconvert between monomer and dimer species PMID: 22789569
    10. The mitochondrial fission protein Fission 1 homologue (Fis1) conveys an apoptosis signal from the mitochondria to the ER by interacting with Bap31 at the ER and facilitating its cleavage into the pro-apoptotic p20Bap31. PMID: 21183955
    11. we observed interactions between Drp1 and Fis1 in mitochondrial fragmentation induced by increasing mitochondrial calcium PMID: 20428767
    12. hFis1 can bind to multiple amino acid sequences selectively, and the tetratricopeptide repeat constitutes the main binding region of hFis1. PMID: 19864424
    13. hFis1 is part of the mammalian fission machinery and regulation of the fission processes might be involved in apoptotic mechanisms PMID: 12783892
    14. regulates mitochondrial fission in mammalian cells through an interaction with the dynamin-like protein DLP1 PMID: 12861026
    15. Cytosolic domain of fis1 adopts a tetratricopeptide repeat domain fold. PMID: 14705031
    16. the level of Fis1 at the mitochondrial surface influences mitochondrial fission events and hence overall mitochondrial morphology within the cell PMID: 14996942
    17. hFis1 induces mitochondrial fragmentation and perinuclear clustering in a state of calcium homeostasis PMID: 15024001
    18. Fis1 together with dynamin-related protein (Drp1) antogonizes Bcl-2. PMID: 16010987
    19. These findings provide the first evidence for a role of Fis1 in peroxisomal fission and suggest that the fission machinery of mitochondria and peroxisomes shares common components. PMID: 16107562
    20. TPR region of hFis1 participates in the interaction with DLP1 or DLP1-containing complex and that the first helix (alpha1) of hFis1 is required for mitochondrial fission presumably by regulating DLP1-hFis1 interaction. PMID: 16118244
    21. Direct induction of mitochondrial elongation by blocking mitochondrial fission process with Fis1-DeltaTM or Drp1-K38A was sufficient to develop senescent phenotypes with increased Reactive oxygen species production. PMID: 16883569
    22. hFis1 is a bifunctional protein that independently regulates mitochondrial fragmentation and endoplasmic reticulum-mediated apoptosis. PMID: 16914522
    23. Fis1 plays important roles in peroxisome division and maintenance of peroxisome morphology in mammalian cells, possibly in a concerted manner with Pex11pbeta and DLP1. PMID: 17408615
    24. hFis1 and OPA1 modulate cellular senescence PMID: 17545159
    25. the concave surface of the Fis1 tetratricopeptide repeat-like domain is evolutionarily conserved to bind the dynamin-like GTPase Dnm1 and not Mdv1 as previously predicted PMID: 17884824
    26. Changes correlate with mitochondrial dysfunction rather than with fragmentation, as substantiated by Fis1 mutants with different effects on organelle shape and function PMID: 18515060
    27. targeting of hFis1 to peroxisomes and mitochondria are independent events and support a direct, Pex19p-dependent targeting of peroxisomal tail-anchored proteins. PMID: 18782765
    28. Fis1 and Mfn1 activities influence mitochondrial signal generation thereby insulin exocytosis. PMID: 18832378
    29. oligomerization of hFis1 in the mitochondrial outer membrane plays a role in mitochondrial fission, potentially through participating in fission factor recruitment. PMID: 18845145

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  • 亚细胞定位：
    Mitochondrion outer membrane; Single-pass membrane protein. Peroxisome membrane; Single-pass membrane protein.
  • 蛋白家族：
    FIS1 family
  • 数据库链接：

    HGNC: 21689

    OMIM: 609003

    KEGG: hsa:51024

    STRING: 9606.ENSP00000223136

    UniGene: Hs.423968