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FIS1 Antibody

  • 货号：
    CSB-PA008684LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA008684LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescent analysis of Hela cells using CSB-PA008684LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 其他：

产品详情

  • 产品描述：

    The FIS1 antibody is a premium polyclonal antibody suitable for the detection of the human FIS1 protein. It is available as the unconjugated IgG isoform. This FIS1 antibody is raised in the rabbit against a partial recombinant protein corresponding to amino acids 1-122 of human FIS1. This FIS1 antibody is purified by protein G affinity chromatography with a purity of up to 95%. And it is recommended for use in ELISA, IHC, and IF applications.

    Its target protein FIS1 mainly functions to regulate mitochondrial fission, morphology, and dynamics. It is involved in the recruitment of DRP1 to the mitochondrial outer membrane and plays a crucial role in mitochondrial quality control and energy metabolism. Dysregulation of FIS1 can contribute to mitochondrial dysfunction and various diseases.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) FIS1 Polyclonal antibody
  • Uniprot No.：
    Q9Y3D6
  • 基因名：
  • 别名：
    2010003O14Rik antibody; CGI 135 antibody; CGI135 antibody; FIS 1 antibody; FIS1 antibody; Fis1 homolog antibody; FIS1; S. cerevisiae; homolog of antibody; FIS1_HUMAN antibody; Fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) antibody; Fission 1 (mitochondrial outer membrane) homolog (yeast) antibody; Fission 1 (mitochondrial outer membrane) homolog antibody; Fission 1 homolog antibody; H NH0132A01.6 antibody; hFis 1 antibody; hFis1 antibody; Mitochondrial fission 1 protein antibody; mitochondrial fission molecule antibody; Tetratricopeptide repeat domain 11 antibody; Tetratricopeptide repeat protein 11 antibody; TPR repeat protein 11 antibody; TTC 11 antibody; TTC11 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Mitochondrial fission 1 protein (1-122AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，FIS1 Antibody (CSB-PA008684LA01HU)，的标记方式是Non-conjugated。对于FIS1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA008684LB01HU FIS1 Antibody, HRP conjugated ELISA
    FITC CSB-PA008684LC01HU FIS1 Antibody, FITC conjugated
    Biotin CSB-PA008684LD01HU FIS1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:500-1:1000
    IF 1:50-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.
  • 基因功能参考文献：
    1. Fis1 depletion in osteoarthritis impairs chondrocyte survival and peroxisomal and lysosomal function. PMID: 27497958
    2. Results show that Fis1 is significantly overexpressed in malignant oncocytomas. PMID: 25822260
    3. Study revealed that a novel mitochondrial fission pathway composed of miR-483-5p and FIS1 regulates cisplatin sensitivity. PMID: 25843291
    4. Overexpression of FoxM1 and Plk1 in Fis1-depleted cells restores mitotic entry. PMID: 23907611
    5. Fis1 expression is significantly increased in spinal cord in a transgenic mouse model of amyotrophic lateral sclerosis. PMID: 23713734
    6. Fis1 acts as a mitochondrial recruitment factor for TBC1D15 that is involved in regulation of mitochondrial morphology. PMID: 23077178
    7. PEX11 proteins attract both Mff and human Fis1 (hFis1) to their site of action. PMID: 22595523
    8. Fis1 and dynamin-related protein (Drp)1 levels are remarkably increased in peripheral blood lymphocytes of Alzheimer's disease patients. PMID: 22340708
    9. Fis1 activity depends on its ability to interconvert between monomer and dimer species PMID: 22789569
    10. The mitochondrial fission protein Fission 1 homologue (Fis1) conveys an apoptosis signal from the mitochondria to the ER by interacting with Bap31 at the ER and facilitating its cleavage into the pro-apoptotic p20Bap31. PMID: 21183955
    11. we observed interactions between Drp1 and Fis1 in mitochondrial fragmentation induced by increasing mitochondrial calcium PMID: 20428767
    12. hFis1 can bind to multiple amino acid sequences selectively, and the tetratricopeptide repeat constitutes the main binding region of hFis1. PMID: 19864424
    13. hFis1 is part of the mammalian fission machinery and regulation of the fission processes might be involved in apoptotic mechanisms PMID: 12783892
    14. regulates mitochondrial fission in mammalian cells through an interaction with the dynamin-like protein DLP1 PMID: 12861026
    15. Cytosolic domain of fis1 adopts a tetratricopeptide repeat domain fold. PMID: 14705031
    16. the level of Fis1 at the mitochondrial surface influences mitochondrial fission events and hence overall mitochondrial morphology within the cell PMID: 14996942
    17. hFis1 induces mitochondrial fragmentation and perinuclear clustering in a state of calcium homeostasis PMID: 15024001
    18. Fis1 together with dynamin-related protein (Drp1) antogonizes Bcl-2. PMID: 16010987
    19. These findings provide the first evidence for a role of Fis1 in peroxisomal fission and suggest that the fission machinery of mitochondria and peroxisomes shares common components. PMID: 16107562
    20. TPR region of hFis1 participates in the interaction with DLP1 or DLP1-containing complex and that the first helix (alpha1) of hFis1 is required for mitochondrial fission presumably by regulating DLP1-hFis1 interaction. PMID: 16118244
    21. Direct induction of mitochondrial elongation by blocking mitochondrial fission process with Fis1-DeltaTM or Drp1-K38A was sufficient to develop senescent phenotypes with increased Reactive oxygen species production. PMID: 16883569
    22. hFis1 is a bifunctional protein that independently regulates mitochondrial fragmentation and endoplasmic reticulum-mediated apoptosis. PMID: 16914522
    23. Fis1 plays important roles in peroxisome division and maintenance of peroxisome morphology in mammalian cells, possibly in a concerted manner with Pex11pbeta and DLP1. PMID: 17408615
    24. hFis1 and OPA1 modulate cellular senescence PMID: 17545159
    25. the concave surface of the Fis1 tetratricopeptide repeat-like domain is evolutionarily conserved to bind the dynamin-like GTPase Dnm1 and not Mdv1 as previously predicted PMID: 17884824
    26. Changes correlate with mitochondrial dysfunction rather than with fragmentation, as substantiated by Fis1 mutants with different effects on organelle shape and function PMID: 18515060
    27. targeting of hFis1 to peroxisomes and mitochondria are independent events and support a direct, Pex19p-dependent targeting of peroxisomal tail-anchored proteins. PMID: 18782765
    28. Fis1 and Mfn1 activities influence mitochondrial signal generation thereby insulin exocytosis. PMID: 18832378
    29. oligomerization of hFis1 in the mitochondrial outer membrane plays a role in mitochondrial fission, potentially through participating in fission factor recruitment. PMID: 18845145

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  • 亚细胞定位：
    Mitochondrion outer membrane; Single-pass membrane protein. Peroxisome membrane; Single-pass membrane protein.
  • 蛋白家族：
    FIS1 family
  • 数据库链接：

    HGNC: 21689

    OMIM: 609003

    KEGG: hsa:51024

    STRING: 9606.ENSP00000223136

    UniGene: Hs.423968