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nos Antibody

  • 中文名称：
    nos兔多克隆抗体
  • 货号：
    CSB-PA340723XA01DLU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: recombinant protein
      All lanes: nos Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 38 kDa
      Observed band size: 38 kDa
  • 其他：

产品详情

  • Uniprot No.：
    P25724
  • 基因名：
    nos
  • 别名：
    Protein nanos nos CG5637
  • 反应种属：
    Drosophila melanogaster (Fruit fly)
  • 免疫原：
    Recombinant Drosophila melanogaster (Fruit fly) nos protein (211-401aa)
  • 免疫原种属：
    Drosophila melanogaster (Fruit fly)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Affinity-chromatography
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Maternal RNA-binding protein that is required for germ cells proliferation and self-renewal. Acts by forming a complex with pum and brat that regulates translation and mRNA stability. The complex binds to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR and prevents its translation. Controls posterior development. Rescuing factor for the abdominal defect of posterior group mutants. The other posterior group genes are not required for nanos function but rather play a role in localization or distribution of nanos protein.
  • 基因功能参考文献：
    1. Nos protein has dual functions in translational repression and stabilization of specific RNAs to ensure proper germline development. PMID: 29278271
    2. germ stem cell-rescuing activity of Wolbachia TomO protein appears to be tightly coupled to the ability to enhance Nos protein expression PMID: 29701280
    3. TomO enhances the maintenance of germ stem cells (GSCs) by elevating Nanos (Nos) expression via its interaction with nos mRNA, ultimately leading to the restoration of germ cell production in Sxl mutant females that are otherwise without GSCs.[ PMID: 27498563
    4. Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch.Bel is required for nos mRNA translational repression. PMID: 28701521
    5. Results showed that nos mRNA, one of the target mRNAs of Hrp38 identified in this study, is regulated by Hrp38 for translational control during ovary development. PMID: 27402862
    6. The results of this experimentat showed that dynein motor machinery components mediate transport of nos mRNA in proximal dendrites. PMID: 24027279
    7. Mei-P26 associates with Bam, Bgcn and Sxl and nanos mRNA during early germline stem cell development, suggesting that this protein helps to repress the translation of nanos mRNA. PMID: 23526974
    8. nanos is a critical regulatory target of polar granule component in primordial germ cells. PMID: 23173091
    9. results suggest that Sxl enables the switch from germline stem cell to committed daughter cell by posttranscriptional down-regulation of nanos expression PMID: 22645327
    10. show that, during late oogenesis, nos translation is also repressed post-initiation and provide evidence that Glo mediates this event PMID: 21460235
    11. The minimal promoter (from -108 to +20) of Nanos is sufficient in the germline stem cells for the green fluorescent protein expression in transgenic Drosophila. PMID: 20026207
    12. Ectopic expression of the Translational Control Element of nos indicates that a common translational control mechanism functions in axial patterning and neuroendocrine signaling in Drosophila. PMID: 12091303
    13. removing Nanos from either germline stem cells (GSCs) or their precursors, PGCs, causes both cell types to differentiate into germline cysts; Nanos is essential for both establishing and maintaining GSCs by preventing their precocious entry into oogenesis PMID: 14976263
    14. Nos establishes germ-line/soma dichotomy and is also required to maintain germ-line fate. PMID: 15240884
    15. analysis of a translational control element in the nanos mRNA PMID: 15525666
    16. Nanos downregulates transcription and modulates CTD phosphorylation during Drosophila development PMID: 15817222
    17. The nanos translational control element represses translation in somatic cells by a Bearded box-like motif. PMID: 15936341
    18. Required to regulate the formation of the germ cell-specific spectrosome, further supporting a role for nos in promoting germ cell identity. PMID: 16028275
    19. Smaug response elements contained in the nanos 3'-untranslated region are necessary and sufficient to induce deadenylation PMID: 16793774
    20. Rumpelstiltskin regulates anterior-posterior axis patterning by functioning as a direct-acting nanos mRNA localization factor. PMID: 18234721
    21. Localization and translational control of nos mRNA are essential for dendritic arborization neuron morphogenesis. PMID: 18472422
    22. These observations suggest that Bam-Bgcn act together to antagonize Nos expression; thus, derepressing cystoblast-promoting factors; findings emphasize the importance of translational repression in balancing stem cell self-renewal and differentiation. PMID: 19470484

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  • 亚细胞定位：
    Cytoplasm, Cytoplasmic ribonucleoprotein granule.
  • 蛋白家族：
    Nanos family
  • 组织特异性：
    Posterior part of the embryo. While the transcript is present throughout the embryo, nos translation is controlled by smg, and the protein is found in pole plasm and pole cells.
  • 数据库链接：

    KEGG: dme:Dmel_CG5637

    STRING: 7227.FBpp0083146

    UniGene: Dm.1485