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  4. Recombinant Drosophila melanogaster Protein nanos (nos)

Recombinant Drosophila melanogaster Protein nanos (nos)

  • 中文名称：
    黑腹果蝇nos重组蛋白
  • 货号：
    CSB-YP340723DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇nos重组蛋白
  • 货号：
    CSB-EP340723DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇nos重组蛋白
  • 货号：
    CSB-EP340723DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇nos重组蛋白
  • 货号：
    CSB-BP340723DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇nos重组蛋白
  • 货号：
    CSB-MP340723DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    nos
  • Uniprot No.：
  • 别名：
    nos; CG5637; Protein nanos
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-401
  • 氨基酸序列
    MFRSNLEGSG AAAVGVANPP SLAQSGKIFQ LQDNFSAFHA RGGLNILGLQ DMYLDTSGAN SSATLSPPIT PVTPDPSTSA QSTHFPFLAD SAATANSLLM QRQYHYHLLL QQQQQLAMAQ HQLALAASAA AASASHQQTD EIARSLKIFA QVTTGAAENA AGSMQDVMQE FATNGYASDD LGRMSYGSAP PQVQMPPQQQ HQQQQGLHLP LGRNPAQLQT NGGNLMPIPL ATHWLNNYRE HLNNVWRNMS YIPAAPNTMG LQAQTAATVS TNLGVGMGLG LPVQGEQLRG ASNSSNNNNN NNKVYKRYNS KAKEISRHCV FCENNNEPEA VINSHSVRDN FNRVLCPKLR TYVCPICGAS GDSAHTIKYC PKKPIITMED AIKAESFRLA KSSYYKQQMK V
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Maternal RNA-binding protein that is required for germ cells proliferation and self-renewal. Acts by forming a complex with pum and brat that regulates translation and mRNA stability. The complex binds to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR and prevents its translation. Controls posterior development. Rescuing factor for the abdominal defect of posterior group mutants. The other posterior group genes are not required for nanos function but rather play a role in localization or distribution of nanos protein.
  • 基因功能参考文献：
    1. Nos protein has dual functions in translational repression and stabilization of specific RNAs to ensure proper germline development. PMID: 29278271
    2. germ stem cell-rescuing activity of Wolbachia TomO protein appears to be tightly coupled to the ability to enhance Nos protein expression PMID: 29701280
    3. TomO enhances the maintenance of germ stem cells (GSCs) by elevating Nanos (Nos) expression via its interaction with nos mRNA, ultimately leading to the restoration of germ cell production in Sxl mutant females that are otherwise without GSCs.[ PMID: 27498563
    4. Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch.Bel is required for nos mRNA translational repression. PMID: 28701521
    5. Results showed that nos mRNA, one of the target mRNAs of Hrp38 identified in this study, is regulated by Hrp38 for translational control during ovary development. PMID: 27402862
    6. The results of this experimentat showed that dynein motor machinery components mediate transport of nos mRNA in proximal dendrites. PMID: 24027279
    7. Mei-P26 associates with Bam, Bgcn and Sxl and nanos mRNA during early germline stem cell development, suggesting that this protein helps to repress the translation of nanos mRNA. PMID: 23526974
    8. nanos is a critical regulatory target of polar granule component in primordial germ cells. PMID: 23173091
    9. results suggest that Sxl enables the switch from germline stem cell to committed daughter cell by posttranscriptional down-regulation of nanos expression PMID: 22645327
    10. show that, during late oogenesis, nos translation is also repressed post-initiation and provide evidence that Glo mediates this event PMID: 21460235
    11. The minimal promoter (from -108 to +20) of Nanos is sufficient in the germline stem cells for the green fluorescent protein expression in transgenic Drosophila. PMID: 20026207
    12. Ectopic expression of the Translational Control Element of nos indicates that a common translational control mechanism functions in axial patterning and neuroendocrine signaling in Drosophila. PMID: 12091303
    13. removing Nanos from either germline stem cells (GSCs) or their precursors, PGCs, causes both cell types to differentiate into germline cysts; Nanos is essential for both establishing and maintaining GSCs by preventing their precocious entry into oogenesis PMID: 14976263
    14. Nos establishes germ-line/soma dichotomy and is also required to maintain germ-line fate. PMID: 15240884
    15. analysis of a translational control element in the nanos mRNA PMID: 15525666
    16. Nanos downregulates transcription and modulates CTD phosphorylation during Drosophila development PMID: 15817222
    17. The nanos translational control element represses translation in somatic cells by a Bearded box-like motif. PMID: 15936341
    18. Required to regulate the formation of the germ cell-specific spectrosome, further supporting a role for nos in promoting germ cell identity. PMID: 16028275
    19. Smaug response elements contained in the nanos 3'-untranslated region are necessary and sufficient to induce deadenylation PMID: 16793774
    20. Rumpelstiltskin regulates anterior-posterior axis patterning by functioning as a direct-acting nanos mRNA localization factor. PMID: 18234721
    21. Localization and translational control of nos mRNA are essential for dendritic arborization neuron morphogenesis. PMID: 18472422
    22. These observations suggest that Bam-Bgcn act together to antagonize Nos expression; thus, derepressing cystoblast-promoting factors; findings emphasize the importance of translational repression in balancing stem cell self-renewal and differentiation. PMID: 19470484

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  • 亚细胞定位：
    Cytoplasm, Cytoplasmic ribonucleoprotein granule.
  • 蛋白家族：
    Nanos family
  • 组织特异性：
    Posterior part of the embryo. While the transcript is present throughout the embryo, nos translation is controlled by smg, and the protein is found in pole plasm and pole cells.
  • 数据库链接：

    KEGG: dme:Dmel_CG5637

    STRING: 7227.FBpp0083146

    UniGene: Dm.1485