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SUP45 Antibody

  • 货号：
    CSB-PA007840XA01SVG
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: recombinant protein
      All lanes: SUP45 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 32 kDa
      Observed band size: 32 kDa
  • 其他：

产品详情

  • Uniprot No.：
    P12385
  • 基因名：
    SUP45
  • 别名：
    Eukaryotic peptide chain release factor subunit 1 (Eukaryotic release factor 1) (eRF1) (Omnipotent suppressor protein 1) SUP45 SAL4 SUP1 YBR143C YBR1120
  • 反应种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 免疫原：
    Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SUP45 protein (26-262aa)
  • 免疫原种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Affinity-chromatography
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.
  • 基因功能参考文献：
    1. We demonstrated that interaction of [SWI+] and [PIN+] causes inactivation of SUP45 gene that leads to nonsense suppression. Our data show that prion interactions may cause heritable traits in Saccharomyces cerevisiae. PMID: 28027291
    2. In this work, the s show that nonsense and missense mutations in the SUP45, but not the SUP35, gene abolish diploid pseudohyphal and haploid invasive growth. PMID: 26054854
    3. Analysis of the L123 systematic mutants of eRF1 suggested that this amino acid functions in stop codon discrimination in a manner coupled with eRF3 binding, and distinctive from previously reported adjacent residues. PMID: 25897120
    4. We characterized a region of the eRF1 N-terminal domain, the P1 pocket, that we had previously shown to be involved in termination efficiency. We identified two residues, arginine 65 and lysine 109, as critical for recognition of the three stop codons. PMID: 25735746
    5. Prion-like determinant [NSI+] decreases the relative amounts of SUP45 mRNA that causes a decrease in the amounts of Sup45 protein. PMID: 25842864
    6. HLJ1 and TEF2 genes are affecting Sup35 protein prionization and/or the efficiency of translation termination. PMID: 24466750
    7. A deterministic mathematical model of the eRF1 (SUP45) feedback loop was developed using a staged increase in model complexity. PMID: 23104998
    8. The results indicated that changes near cavities two and three frequently mediated significant effects on stop codon selectivity by eRF1 (SUP45). Changes in the YCF motif correlated most consistently with variant code stop codon selectivity. PMID: 22543865
    9. Deletion of each UPF gene leads to allosuppresson of ade1-14 mutation without changing eRF1 amount PMID: 22670525
    10. SUP45 gene modulates nonsense suppression in S. cerevisiae PMID: 22215010
    11. The role of translation termination factor eRF1 in the regulation of pseudohyphal growth in Saccharomyces cerevisiae cells. PMID: 20714858
    12. Results indicate that point mutants of Saccharomyces cerevisiae eRF1 display significant variability in their stop codon read-through phenotypes. PMID: 20444877
    13. Overexpression of genes encoding tRNA(Tyr) AND tRNA(Gln) improves viability of nonsense mutants in SUP45 gene in yeast Saccharomyces cerevisiae PMID: 20586191
    14. Mtq2p methylates Sup45p PMID: 16321977
    15. sup45 mutations do not only change translation fidelity but also acts by causing a change in mRNA stability PMID: 17705828
    16. The results of this study suggest that the N-terminal eRF1 fragment is indispensable for cell viability of nonsense mutants due to the involvement in termination of translation. PMID: 18069340
    17. The data suggest that increasing stop codon suppression and decreasing of the binding affinity of eRF1(Y410S) were probably due to the substitution of tyrosine to serine which triggered the structural change on the C-terminal domain PMID: 18463713
    18. mutagenesis of SUP45 led to the identification of two new pockets in domain 1 (P1 and P2) involved in the regulation of eRF1 activity. PMID: 19174561
    19. compared readthrough efficiency of the natural termination codon of SUP45 gene, spontaneous sup45-n (nonsense) mutations, nonsense mutations obtained by site-directed mutagenesis (76Q --> TAA, 242R --> TGA, 317L --> TAG) PMID: 19370360

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Eukaryotic release factor 1 family
  • 数据库链接：

    KEGG: sce:YBR143C

    STRING: 4932.YBR143C