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  4. Recombinant Saccharomyces cerevisiae Eukaryotic peptide chain release factor subunit 1 (SUP45)

Recombinant Saccharomyces cerevisiae Eukaryotic peptide chain release factor subunit 1 (SUP45)

  • 货号：
    CSB-YP007840SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007840SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007840SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007840SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007840SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SUP45
  • Uniprot No.：
  • 别名：
    SUP45; SAL4; SUP1; YBR143C; YBR1120Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; eRF1; Omnipotent suppressor protein 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-437
  • 氨基酸序列
    MDNEVEKNIE IWKVKKLVQS LEKARGNGTS MISLVIPPKG QIPLYQKMLT DEYGTASNIK SRVNRLSVLS AITSTQQKLK LYNTLPKNGL VLYCGDIITE DGKEKKVTFD IEPYKPINTS LYLCDNKFHT EVLSELLQAD DKFGFIVMDG QGTLFGSVSG NTRTVLHKFT VDLPKKHGRG GQSALRFARL REEKRHNYVR KVAEVAVQNF ITNDKVNVKG LILAGSADFK TDLAKSELFD PRLACKVISI VDVSYGGENG FNQAIELSAE ALANVKYVQE KKLLEAYFDE ISQDTGKFCY GIDDTLKALD LGAVEKLIVF ENLETIRYTF KDAEDNEVIK FAEPEAKDKS FAIDKATGQE MDVVSEEPLI EWLAANYKNF GATLEFITDK SSEGAQFVTG FGGIGAMLRY KVNFEQLVDE SEDEYYDEDE GSDYDFI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.
  • 基因功能参考文献：
    1. We demonstrated that interaction of [SWI+] and [PIN+] causes inactivation of SUP45 gene that leads to nonsense suppression. Our data show that prion interactions may cause heritable traits in Saccharomyces cerevisiae. PMID: 28027291
    2. In this work, the s show that nonsense and missense mutations in the SUP45, but not the SUP35, gene abolish diploid pseudohyphal and haploid invasive growth. PMID: 26054854
    3. Analysis of the L123 systematic mutants of eRF1 suggested that this amino acid functions in stop codon discrimination in a manner coupled with eRF3 binding, and distinctive from previously reported adjacent residues. PMID: 25897120
    4. We characterized a region of the eRF1 N-terminal domain, the P1 pocket, that we had previously shown to be involved in termination efficiency. We identified two residues, arginine 65 and lysine 109, as critical for recognition of the three stop codons. PMID: 25735746
    5. Prion-like determinant [NSI+] decreases the relative amounts of SUP45 mRNA that causes a decrease in the amounts of Sup45 protein. PMID: 25842864
    6. HLJ1 and TEF2 genes are affecting Sup35 protein prionization and/or the efficiency of translation termination. PMID: 24466750
    7. A deterministic mathematical model of the eRF1 (SUP45) feedback loop was developed using a staged increase in model complexity. PMID: 23104998
    8. The results indicated that changes near cavities two and three frequently mediated significant effects on stop codon selectivity by eRF1 (SUP45). Changes in the YCF motif correlated most consistently with variant code stop codon selectivity. PMID: 22543865
    9. Deletion of each UPF gene leads to allosuppresson of ade1-14 mutation without changing eRF1 amount PMID: 22670525
    10. SUP45 gene modulates nonsense suppression in S. cerevisiae PMID: 22215010
    11. The role of translation termination factor eRF1 in the regulation of pseudohyphal growth in Saccharomyces cerevisiae cells. PMID: 20714858
    12. Results indicate that point mutants of Saccharomyces cerevisiae eRF1 display significant variability in their stop codon read-through phenotypes. PMID: 20444877
    13. Overexpression of genes encoding tRNA(Tyr) AND tRNA(Gln) improves viability of nonsense mutants in SUP45 gene in yeast Saccharomyces cerevisiae PMID: 20586191
    14. Mtq2p methylates Sup45p PMID: 16321977
    15. sup45 mutations do not only change translation fidelity but also acts by causing a change in mRNA stability PMID: 17705828
    16. The results of this study suggest that the N-terminal eRF1 fragment is indispensable for cell viability of nonsense mutants due to the involvement in termination of translation. PMID: 18069340
    17. The data suggest that increasing stop codon suppression and decreasing of the binding affinity of eRF1(Y410S) were probably due to the substitution of tyrosine to serine which triggered the structural change on the C-terminal domain PMID: 18463713
    18. mutagenesis of SUP45 led to the identification of two new pockets in domain 1 (P1 and P2) involved in the regulation of eRF1 activity. PMID: 19174561
    19. compared readthrough efficiency of the natural termination codon of SUP45 gene, spontaneous sup45-n (nonsense) mutations, nonsense mutations obtained by site-directed mutagenesis (76Q --> TAA, 242R --> TGA, 317L --> TAG) PMID: 19370360

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Eukaryotic release factor 1 family
  • 数据库链接：

    KEGG: sce:YBR143C

    STRING: 4932.YBR143C