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SPRY4 Antibody

  • 货号：
    CSB-PA866335LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunofluorescence staining of PC-3 cells with CSB-PA866335LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) SPRY4 Polyclonal antibody
  • Uniprot No.：
    Q9C004
  • 基因名：
    SPRY4
  • 别名：
    Protein sprouty homolog 4 antibody; Sprouty 4 antibody; Sprouty homolog 4 antibody; sprouty homolog 4 (Drosophila) antibody; SPRY 4 antibody; Spry-4 antibody; Spry4 antibody; SPY4_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Protein sprouty homolog 4 protein (1-299AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，SPRY4 Antibody (CSB-PA866335LA01HU)，的标记方式是Non-conjugated。对于SPRY4 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA866335LB01HU SPRY4 Antibody, HRP conjugated ELISA
    FITC CSB-PA866335LC01HU SPRY4 Antibody, FITC conjugated
    Biotin CSB-PA866335LD01HU SPRY4 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IF 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1. Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin.
  • 基因功能参考文献：
    1. The findings indicate that SPRY4 and SPRY4-IT1 may act as oncogenes in testicular germ cell tumors via activation of the PI3K/Akt signaling pathway. PMID: 29410498
    2. Results show that SPRY4 expression level was significantly decreased in glioma tissues, associated with short survival time, and a key target gene of miR-1908. PMID: 29048686
    3. Studied BAK1, SPRY4 and GAB2 SNPs in pediatric germ cell tumors(GCT); found a variant in SPRY4 was associated with reduced risk of GCT; a variant in BAK1 was positively associated with GCT with a strong estimated effect for testis tumors; and a SNP in GAB2 was associated with increased risk for GCT. PMID: 28295819
    4. results found that histone methylation mediated by CCAT1 could also contribute to the lower expression of SPRY4 in esophageal squamous cell carcinoma PMID: 27956498
    5. mechanistic evidence that KSRP promotes the down-regulation of Spry4 by a previously unidentified mechanism, i.e. post-transcriptional mRNA regulation. PMID: 28275056
    6. Polymorphisms of KITLG, SPRY4, and BAK1 genes in patients with testicular germ cell tumors and individuals with infertility associated with AZFc deletion of the Y chromosome PMID: 28064312
    7. SPRY4 is involved in the development and progression of colorectal cancer. PMID: 27997895
    8. Small interfering RNA (siRNA)-mediated knockdown of SPRY4 attenuated the AREG-induced down-regulation of E-cadherin. PMID: 26768617
    9. These findings suggest that SPRY4-IT1 plays a direct role in the regulation of metastasis and progression of osteosarcoma. PMID: 26982001
    10. Results identify the tumor suppressor SPRY4 as a novel molecular effector of MT1-MMP affecting melanoma cell motility. PMID: 26392417
    11. Data show that proto-oncogene protein B-raf (BRAF) inhibition induces c-Jun N-terminal kinase (c-JUN) expression and c-JUN abundance and activation by down-regulating SPRY2/4 protein expression. PMID: 26286024
    12. Specific protein 1 plays a crucial role in the regulation of Sprouty4 in response to serum. PMID: 25957915
    13. SPRY4 is often deleted in secondary acute myeloid leukemia. PMID: 26204823
    14. Sprouty 2 protein, but not Sprouty 4, is an independent prognostic biomarker for human epithelial ovarian cancer. PMID: 25630587
    15. these results suggest the possibility that SPRY4-IT1 knockdown may induce apoptosis via lipin 2-mediated alterations in lipid metabolism leading to cellular lipotoxicity. PMID: 25344859
    16. SPRY4 may function as a tumor suppressor in endometrial adenocarcinoma. PMID: 24811094
    17. demonstrated that knockdown of SPRY4-IT1 reduced cell proliferation, invasiveness, and migration PMID: 24810925
    18. Overexpression of Sprouty4 or pharmacological inhibition of ERK upregulated IL-1R1 expression in primary T cells.Sprouty4 and ERK play a critical role in developing iTh17 cells in Th17 cell-driven autoimmune diseases. PMID: 24732356
    19. Sprouty4 expression negatively influences cell migration. PMID: 24402575
    20. Overexpressed Spry4 in endothelial cells inhibited migration and vitronectin adhesion. Spry4 knockdown enhanced these. Overexpression decreased integrin beta3 protein post-transcriptionally by modulating its tyrosine phosphorylation by c-Src. PMID: 23955631
    21. results validated previously reported genetic associations with testicular germ cell tumor in a Scandinavian population, and observed suggestive evidence of a parent-of-origin effect in SPRY4. PMID: 23640991
    22. Sprouty2 (but not Sprouty4) has a role in inhibition of cell proliferation and migration of osteosarcoma cells PMID: 23831057
    23. Spry1 and Spry4 have opposing roles in VSMC phenotypic modulation, and Spry1 maintains the VSMC differentiation phenotype in vitro in part through an Akt/FoxO/myocardin pathway. PMID: 23554919
    24. siRNA-mediated knockdown of Spry1, Spry2, or Spry4 promotes IFN-inducible antileukemic effects in vitro and results in enhanced suppressive effects on malignant hematopoietic progenitors from patients with polycythemia vera. PMID: 23074222
    25. The SPRY4 (rs4324715) variants were significantly associated with germ cell tumors only in the adolescent age group. PMID: 22072546
    26. Spry4 is a downstream target of Wnt7A/Fzd 9 signaling and may have efficacy in the treatment of non-small cell lung cancer. PMID: 20501643
    27. The Spry4 might be involved in the timely restriction of MAPK signals under hypoxic conditions, similar to its role in mitogen-regulated processes. PMID: 20482313
    28. interacts with the dual specificity kinase TESK1; maps to 5q31.3 PMID: 12027893
    29. Sprouty4 suppresses vascular epithelial growth factor (VEGF)-induced, Ras-independent activation of Raf1 but does not affect epidermal growth factor (EGF)-induced, Ras-dependent activation of Raf1. PMID: 12717443
    30. Core promoter activity is located within proximal 0.4-kb region. Minimal ERK-inducible promoter activity is between -69 and -31. Core promoter region of hSpry4 gene exhibits significant homology to 5'-flanking region of mouse gene. PMID: 14977631
    31. Sprouty4 expression is down regulated in human prostate cancer by DNA methylation and this decreased expression may contribute to increased cell migration. PMID: 16388505
    32. Human, chimpanzee, rat and mouse SPRY4 orthologs, consisting of three exons, were well conserved PMID: 16465403
    33. the Sprouty/Caveolin-1 interaction modulates signaling in a growth factor- and Sprouty isoform-specific manner PMID: 16877379
    34. SPRY4 gene was found to be associated with schizophrenia. PMID: 18298822
    35. These results demonstrate that common genetic variants affect TGCT risk and implicate KITLG and SPRY4 as genes involved in Testicular germ cell tumors susceptibility. PMID: 19483682
    36. cell adaptation to activated FGFR3 include Sprouty4 activity, which silences the premature receptor signaling and suppress apoptosis. PMID: 19761767

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  • 相关疾病：
    Hypogonadotropic hypogonadism 17 with or without anosmia (HH17)
  • 亚细胞定位：
    Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Sprouty family
  • 数据库链接：

    HGNC: 15533

    OMIM: 607984

    KEGG: hsa:81848

    STRING: 9606.ENSP00000344967

    UniGene: Hs.323308