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  4. Recombinant Human Protein sprouty homolog 4 (SPRY4)

Recombinant Human Protein sprouty homolog 4 (SPRY4)

  • 中文名称：
    人SPRY4重组蛋白
  • 货号：
    CSB-YP866335HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SPRY4重组蛋白
  • 货号：
    CSB-EP866335HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人SPRY4重组蛋白
  • 货号：
    CSB-EP866335HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SPRY4重组蛋白
  • 货号：
    CSB-BP866335HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人SPRY4重组蛋白
  • 货号：
    CSB-MP866335HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SPRY4
  • Uniprot No.：
  • 别名：
    Protein sprouty homolog 4; Sprouty 4; Sprouty homolog 4; sprouty homolog 4 (Drosophila); SPRY 4; Spry-4; Spry4; SPY4_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-299
  • 氨基酸序列
    MEPPIPQSAP LTPNSVMVQP LLDSRMSHSR LQHPLTILPI DQVKTSHVEN DYIDNPSLAL TTGPKRTRGG APELAPTPAR CDQDVTHHWI SFSGRPSSVS SSSSTSSDQR LLDHMAPPPV ADQASPRAVR IQPKVVHCQP LDLKGPAVPP ELDKHFLLCE ACGKCKCKEC ASPRTLPSCW VCNQECLCSA QTLVNYGTCM CLVQGIFYHC TNEDDEGSCA DHPCSCSRSN CCARWSFMGA LSVVLPCLLC YLPATGCVKL AQRGYDRLRR PGCRCKHTNS VICKAASGDA KTSRPDKPF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1. Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin.
  • 基因功能参考文献：
    1. The findings indicate that SPRY4 and SPRY4-IT1 may act as oncogenes in testicular germ cell tumors via activation of the PI3K/Akt signaling pathway. PMID: 29410498
    2. Results show that SPRY4 expression level was significantly decreased in glioma tissues, associated with short survival time, and a key target gene of miR-1908. PMID: 29048686
    3. Studied BAK1, SPRY4 and GAB2 SNPs in pediatric germ cell tumors(GCT); found a variant in SPRY4 was associated with reduced risk of GCT; a variant in BAK1 was positively associated with GCT with a strong estimated effect for testis tumors; and a SNP in GAB2 was associated with increased risk for GCT. PMID: 28295819
    4. results found that histone methylation mediated by CCAT1 could also contribute to the lower expression of SPRY4 in esophageal squamous cell carcinoma PMID: 27956498
    5. mechanistic evidence that KSRP promotes the down-regulation of Spry4 by a previously unidentified mechanism, i.e. post-transcriptional mRNA regulation. PMID: 28275056
    6. Polymorphisms of KITLG, SPRY4, and BAK1 genes in patients with testicular germ cell tumors and individuals with infertility associated with AZFc deletion of the Y chromosome PMID: 28064312
    7. SPRY4 is involved in the development and progression of colorectal cancer. PMID: 27997895
    8. Small interfering RNA (siRNA)-mediated knockdown of SPRY4 attenuated the AREG-induced down-regulation of E-cadherin. PMID: 26768617
    9. These findings suggest that SPRY4-IT1 plays a direct role in the regulation of metastasis and progression of osteosarcoma. PMID: 26982001
    10. Results identify the tumor suppressor SPRY4 as a novel molecular effector of MT1-MMP affecting melanoma cell motility. PMID: 26392417
    11. Data show that proto-oncogene protein B-raf (BRAF) inhibition induces c-Jun N-terminal kinase (c-JUN) expression and c-JUN abundance and activation by down-regulating SPRY2/4 protein expression. PMID: 26286024
    12. Specific protein 1 plays a crucial role in the regulation of Sprouty4 in response to serum. PMID: 25957915
    13. SPRY4 is often deleted in secondary acute myeloid leukemia. PMID: 26204823
    14. Sprouty 2 protein, but not Sprouty 4, is an independent prognostic biomarker for human epithelial ovarian cancer. PMID: 25630587
    15. these results suggest the possibility that SPRY4-IT1 knockdown may induce apoptosis via lipin 2-mediated alterations in lipid metabolism leading to cellular lipotoxicity. PMID: 25344859
    16. SPRY4 may function as a tumor suppressor in endometrial adenocarcinoma. PMID: 24811094
    17. demonstrated that knockdown of SPRY4-IT1 reduced cell proliferation, invasiveness, and migration PMID: 24810925
    18. Overexpression of Sprouty4 or pharmacological inhibition of ERK upregulated IL-1R1 expression in primary T cells.Sprouty4 and ERK play a critical role in developing iTh17 cells in Th17 cell-driven autoimmune diseases. PMID: 24732356
    19. Sprouty4 expression negatively influences cell migration. PMID: 24402575
    20. Overexpressed Spry4 in endothelial cells inhibited migration and vitronectin adhesion. Spry4 knockdown enhanced these. Overexpression decreased integrin beta3 protein post-transcriptionally by modulating its tyrosine phosphorylation by c-Src. PMID: 23955631
    21. results validated previously reported genetic associations with testicular germ cell tumor in a Scandinavian population, and observed suggestive evidence of a parent-of-origin effect in SPRY4. PMID: 23640991
    22. Sprouty2 (but not Sprouty4) has a role in inhibition of cell proliferation and migration of osteosarcoma cells PMID: 23831057
    23. Spry1 and Spry4 have opposing roles in VSMC phenotypic modulation, and Spry1 maintains the VSMC differentiation phenotype in vitro in part through an Akt/FoxO/myocardin pathway. PMID: 23554919
    24. siRNA-mediated knockdown of Spry1, Spry2, or Spry4 promotes IFN-inducible antileukemic effects in vitro and results in enhanced suppressive effects on malignant hematopoietic progenitors from patients with polycythemia vera. PMID: 23074222
    25. The SPRY4 (rs4324715) variants were significantly associated with germ cell tumors only in the adolescent age group. PMID: 22072546
    26. Spry4 is a downstream target of Wnt7A/Fzd 9 signaling and may have efficacy in the treatment of non-small cell lung cancer. PMID: 20501643
    27. The Spry4 might be involved in the timely restriction of MAPK signals under hypoxic conditions, similar to its role in mitogen-regulated processes. PMID: 20482313
    28. interacts with the dual specificity kinase TESK1; maps to 5q31.3 PMID: 12027893
    29. Sprouty4 suppresses vascular epithelial growth factor (VEGF)-induced, Ras-independent activation of Raf1 but does not affect epidermal growth factor (EGF)-induced, Ras-dependent activation of Raf1. PMID: 12717443
    30. Core promoter activity is located within proximal 0.4-kb region. Minimal ERK-inducible promoter activity is between -69 and -31. Core promoter region of hSpry4 gene exhibits significant homology to 5'-flanking region of mouse gene. PMID: 14977631
    31. Sprouty4 expression is down regulated in human prostate cancer by DNA methylation and this decreased expression may contribute to increased cell migration. PMID: 16388505
    32. Human, chimpanzee, rat and mouse SPRY4 orthologs, consisting of three exons, were well conserved PMID: 16465403
    33. the Sprouty/Caveolin-1 interaction modulates signaling in a growth factor- and Sprouty isoform-specific manner PMID: 16877379
    34. SPRY4 gene was found to be associated with schizophrenia. PMID: 18298822
    35. These results demonstrate that common genetic variants affect TGCT risk and implicate KITLG and SPRY4 as genes involved in Testicular germ cell tumors susceptibility. PMID: 19483682
    36. cell adaptation to activated FGFR3 include Sprouty4 activity, which silences the premature receptor signaling and suppress apoptosis. PMID: 19761767

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  • 相关疾病：
    Hypogonadotropic hypogonadism 17 with or without anosmia (HH17)
  • 亚细胞定位：
    Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Sprouty family
  • 数据库链接：

    HGNC: 15533

    OMIM: 607984

    KEGG: hsa:81848

    STRING: 9606.ENSP00000344967

    UniGene: Hs.323308