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SPRTN Antibody

  • 货号：
    CSB-PA863937LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA863937LA01HU at dilution of 1:100
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) SPRTN Polyclonal antibody
  • Uniprot No.：
    Q9H040
  • 基因名：
    SPRTN
  • 别名：
    SPRTN antibody; C1orf124 antibody; DVC1 antibody; UNQ1880/PRO4323 antibody; SprT-like domain-containing protein Spartan antibody; DNA damage protein targeting VCP antibody; DVC1 antibody; Protein with SprT-like domain at the N terminus antibody; Spartan antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human SprT-like domain-containing protein Spartan protein (1-239AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，SPRTN Antibody (CSB-PA863937LA01HU)，的标记方式是Non-conjugated。对于SPRTN Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA863937LB01HU SPRTN Antibody, HRP conjugated ELISA
    FITC CSB-PA863937LC01HU SPRTN Antibody, FITC conjugated
    Biotin CSB-PA863937LD01HU SPRTN Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    DNA-dependent metalloendopeptidase that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity. DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde. Associates with the DNA replication machinery and specifically removes DPCs during DNA synthesis. Acts as a pleiotropic protease for DNA-binding proteins cross-linked with DNA, such as TOP1, TOP2A, histones H3 and H4. Mediates degradation of DPCs that are not ubiquitinated, while it is not able to degrade ubiquitinated DPCs. SPRTN activation requires polymerase collision with DPCs followed by helicase bypass of DPCs. Involved in recruitment of VCP/p97 to sites of DNA damage. Also acts as an activator of CHEK1 during normal DNA replication by mediating proteolytic cleavage of CHEK1, thereby promoting CHEK1 removal from chromatin and subsequent activation. Does not activate CHEK1 in response to DNA damage. May also act as a 'reader' of ubiquitinated PCNA: recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis.
  • 基因功能参考文献：
    1. e show that SPRTN is a DNA-dependent mammalian protease required for resolving cytotoxic DNA-protein crosslinks (DPCs)- a function that had only been attributed to the metalloprotease Wss1 in budding yeast. We provide genetic evidence that SPRTN and Wss1 function distinctly in vivo to resolve DPCs PMID: 27852435
    2. Loss of SPRTN results in failure to repair DNA-protein crosslinks (DPCs) and hypersensitivity to DPC-inducing agents. PMID: 27871365
    3. SPRTN protease represents a specialized DNA replication-coupled DPC repair pathway essential for DNA replication progression and genome stability. PMID: 27871366
    4. Spartan has a DNA-dependent protease activity degrading certain proteins bound to DNA. PMID: 28053116
    5. DNA binding by SPARTAN regulates the targeting of Poleta to damage sites after UV exposure, and this function contributes highly to its DNA-damage tolerance function. PMID: 27838458
    6. These findings offer new insights into the determinants of PIP box for PCNA binding. PMID: 27084448
    7. USP1, p21 and Spartan are translesion DNA synthesis regulators. (Review) PMID: 26002196
    8. Mutations in SPRTN cause early onset hepatocellular carcinoma, genomic instability and progeroid features. PMID: 25261934
    9. The results suggest that Spartan negatively regulates POLD3 function in Rev1/Pol zeta-dependent translesion synthesis. PMID: 23254330
    10. Spartan is recruited to sites of stalled replication via ubiquitinated PCNA and plays an important role to prevent mutations associated with replication of damaged DNA. PMID: 22894931
    11. s propose that Spartan promotes genomic stability by regulating the choice of rescue of stalled replication fork, whose mechanism includes its interaction with ubiquitin-conjugated PCNA and protection against PCNA deubiquitylation. PMID: 22987070
    12. DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks. PMID: 23042605
    13. DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage. PMID: 23042607
    14. C1orf124 acts at multiple steps in TLS, stabilizes RAD18 and ubiquitinated PCNA at damage sites, and facilitates the switch from replicative to TLS polymerase to bypass DNA lesion. PMID: 22902628
    15. Spartan promotes an unexpected feed-forward loop to enhance PCNA ubiquitylation and translesion DNA synthesis. PMID: 22681887

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  • 相关疾病：
    Ruijs-Aalfs syndrome (RJALS)
  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Spartan family
  • 数据库链接：

    HGNC: 25356

    OMIM: 616086

    KEGG: hsa:83932

    STRING: 9606.ENSP00000295050

    UniGene: Hs.554892