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  4. Recombinant Human Zinc finger RAD18 domain-containing protein C1orf124 (C1orf124)

Recombinant Human Zinc finger RAD18 domain-containing protein C1orf124 (C1orf124)

  • 中文名称：
    人SPRTN重组蛋白
  • 货号：
    CSB-YP863937HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SPRTN重组蛋白
  • 货号：
    CSB-EP863937HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人SPRTN重组蛋白
  • 货号：
    CSB-EP863937HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SPRTN重组蛋白
  • 货号：
    CSB-BP863937HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人SPRTN重组蛋白
  • 货号：
    CSB-MP863937HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SPRTN
  • Uniprot No.：
  • 别名：
    SPRTN; C1orf124; DVC1; UNQ1880/PRO4323; SprT-like domain-containing protein Spartan; DNA damage protein targeting VCP; DVC1; Protein with SprT-like domain at the N terminus; Spartan
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-489
  • 氨基酸序列
    MDDDLMLALR LQEEWNLQEA ERDHAQESLS LVDASWELVD PTPDLQALFV QFNDQFFWGQ LEAVEVKWSV RMTLCAGICS YEGKGGMCSI RLSEPLLKLR PRKDLVETLL HEMIHAYLFV TNNDKDREGH GPEFCKHMHR INSLTGANIT VYHTFHDEVD EYRRHWWRCN GPCQHRPPYY GYVKRATNRE PSAHDYWWAE HQKTCGGTYI KIKEPENYSK KGKGKAKLGK EPVLAAENKD KPNRGEAQLV IPFSGKGYVL GETSNLPSPG KLITSHAINK TQDLLNQNHS ANAVRPNSKI KVKFEQNGSS KNSHLVSPAV SNSHQNVLSN YFPRVSFANQ KAFRGVNGSP RISVTVGNIP KNSVSSSSQR RVSSSKISLR NSSKVTESAS VMPSQDVSGS EDTFPNKRPR LEDKTVFDNF FIKKEQIKSS GNDPKYSTTT AQNSSSSSSQ SKMVNCPVCQ NEVLESQINE HLDWCLEGDS IKVKSEESL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA-dependent metalloendopeptidase that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity. DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde. Associates with the DNA replication machinery and specifically removes DPCs during DNA synthesis. Acts as a pleiotropic protease for DNA-binding proteins cross-linked with DNA, such as TOP1, TOP2A, histones H3 and H4. Mediates degradation of DPCs that are not ubiquitinated, while it is not able to degrade ubiquitinated DPCs. SPRTN activation requires polymerase collision with DPCs followed by helicase bypass of DPCs. Involved in recruitment of VCP/p97 to sites of DNA damage. Also acts as an activator of CHEK1 during normal DNA replication by mediating proteolytic cleavage of CHEK1, thereby promoting CHEK1 removal from chromatin and subsequent activation. Does not activate CHEK1 in response to DNA damage. May also act as a 'reader' of ubiquitinated PCNA: recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis.
  • 基因功能参考文献：
    1. e show that SPRTN is a DNA-dependent mammalian protease required for resolving cytotoxic DNA-protein crosslinks (DPCs)- a function that had only been attributed to the metalloprotease Wss1 in budding yeast. We provide genetic evidence that SPRTN and Wss1 function distinctly in vivo to resolve DPCs PMID: 27852435
    2. Loss of SPRTN results in failure to repair DNA-protein crosslinks (DPCs) and hypersensitivity to DPC-inducing agents. PMID: 27871365
    3. SPRTN protease represents a specialized DNA replication-coupled DPC repair pathway essential for DNA replication progression and genome stability. PMID: 27871366
    4. Spartan has a DNA-dependent protease activity degrading certain proteins bound to DNA. PMID: 28053116
    5. DNA binding by SPARTAN regulates the targeting of Poleta to damage sites after UV exposure, and this function contributes highly to its DNA-damage tolerance function. PMID: 27838458
    6. These findings offer new insights into the determinants of PIP box for PCNA binding. PMID: 27084448
    7. USP1, p21 and Spartan are translesion DNA synthesis regulators. (Review) PMID: 26002196
    8. Mutations in SPRTN cause early onset hepatocellular carcinoma, genomic instability and progeroid features. PMID: 25261934
    9. The results suggest that Spartan negatively regulates POLD3 function in Rev1/Pol zeta-dependent translesion synthesis. PMID: 23254330
    10. Spartan is recruited to sites of stalled replication via ubiquitinated PCNA and plays an important role to prevent mutations associated with replication of damaged DNA. PMID: 22894931
    11. s propose that Spartan promotes genomic stability by regulating the choice of rescue of stalled replication fork, whose mechanism includes its interaction with ubiquitin-conjugated PCNA and protection against PCNA deubiquitylation. PMID: 22987070
    12. DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks. PMID: 23042605
    13. DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage. PMID: 23042607
    14. C1orf124 acts at multiple steps in TLS, stabilizes RAD18 and ubiquitinated PCNA at damage sites, and facilitates the switch from replicative to TLS polymerase to bypass DNA lesion. PMID: 22902628
    15. Spartan promotes an unexpected feed-forward loop to enhance PCNA ubiquitylation and translesion DNA synthesis. PMID: 22681887

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  • 相关疾病：
    Ruijs-Aalfs syndrome (RJALS)
  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Spartan family
  • 数据库链接：

    HGNC: 25356

    OMIM: 616086

    KEGG: hsa:83932

    STRING: 9606.ENSP00000295050

    UniGene: Hs.554892