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  4. Phospho-CAMK4 (Thr196/200) Antibody

Phospho-CAMK4 (Thr196/200) Antibody

  • 货号：
    CSB-PA925801
  • 规格：
    ￥2454
  • 图片：
    • Western blot analysis of extracts from K562 cells treated with H2O2 using Phospho-CaMK4 (Thr196/200) antibody.The lane on the right is treated with the antigen-specific peptide.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CAMK4 Polyclonal antibody
  • Uniprot No.：
    Q16566
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Peptide sequence around phosphorylation site of threonine 200 (M-K-T(p)-V-C) derived from Human CaMK4 or threonine 196 (M-K-T(p)-V-C) derived from Mouse CaMK4.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.
  • 基因功能参考文献：
    1. vanillin binds strongly to the active site cavity of CAMKIV and stabilized by a large number of non-covalent interactions. PMID: 28744811
    2. Genotype and allele frequencies of CAMKIV gene SNPs differed significantly between alcohol dependence patients and control subjects. The results of the present study suggest that CAMKIV might be a candidate alcohol dependence gene. PMID: 28734942
    3. hTau accumulation impairs synapse and memory by CaN-mediated suppression of nuclear CaMKIV/CREB signaling. PMID: 27298345
    4. Within the pH range 5.0-11.5, CAMK4 maintained both its secondary and tertiary structures, along with its function, whereas significant aggregation was observed at acidic pH (2.0-4.5). PMID: 27032767
    5. A positive association was not observed between rs10491334 in the CAMK4 gene and longevity in a Chinese population. PMID: 27659345
    6. The T-allele of rs10491334 in CAMK4 was associated with hypertension in the Uygur group. PMID: 26909912
    7. Expression of CaMKIV inhibits autophosphorylation and activation of CaMKII, and elicits G0/G1cell cycle arrest,impairing cell proliferation. PMID: 25446257
    8. An imbalance of specific isoforms of CYFIP1, an FMRP interaction partner, and CAMK4, a transcriptional regulator of the FMRP gene, modulates risk for autism spectrum disorders. PMID: 24442360
    9. CaMK4-dependent activation of AKT/mTOR and CREM-alpha underlies autoimmunity-associated Th17 imbalance. PMID: 24667640
    10. CaMK4 regulates beta-cell proliferation and apoptosis in a CREB-dependent manner and CaMK4-induced IRS-2 expression is important in these processes PMID: 23049845
    11. study suggests that the mutations in CAMK4 may lead to abnormal semen parameters PMID: 22897820
    12. Phosphorylated Notch1-IC by CaMKIV increases Notch1-IC stability, which enhances osteoclast differentiation. PMID: 23103515
    13. Prolongevity genes are activated by CAMKIV, the levels of which are influenced by rs10491334, a single-nucleotide polymorphism associated with human longevity. PMID: 21612516
    14. The regulation of RORalpha activity by PKA as well as CaMK-IV provides a new link in the signalling network that regulates metabolic processes such as glycogen and lipid metabolism. PMID: 21514275
    15. These findings suggest that PLC/CAMK IV-NF-kappaB is involved in RAGE mediated signaling pathway in human endothelial cells. PMID: 20171262
    16. CaMKIV proteins were found in the nucleus of epithelial ovarian cancer tissue. CaMKIV expression was significantly associated with clinical stage (P<0.01), histological grade (P<0.01), and clinical outcome (P<0.01). PMID: 12065094
    17. sequestration of CaMKK may be the molecular mechanism by which catalytically inactive mutants of CaMKIV exert their "dominant-negative" functions within the cell PMID: 14701808
    18. the Ca(2+)/CaM binding-autoinhibitory domain of CaMKIV is required for association of the kinase with PP2A PMID: 15143065
    19. calcium/CaMKIV signaling pathway may play an important role in the excitation-mediated regulation of corticotropin releasing hormone synthesis PMID: 15591024
    20. the function of CaMK II is essential for PAF-induced macrophage priming, while CaMK IV is not specific for priming by PAF and appears to have a direct link in TLR4-mediated events PMID: 15665723
    21. CaMKIV is expressed in human sperm and may have a role in the regulation of human sperm motility PMID: 15840651
    22. Results identify calcium/calmodulin-dependent kinase IV as being responsible for the increased expression of CREM and the decreased production of interleukin-2 in systemic lupus erythematosus T cells. PMID: 15841182
    23. Transgenic CaMKIV plays a modulatory role in the nucleus accumbens in anxiety-like behavior of adult CaMKIV variant mice. PMID: 18053176
    24. CaMK-4 expression correlates positively with the ability to form long-term memory and implicates the decline of CaMKIV signaling mechanisms in age-related memory deficits. PMID: 18829949
    25. CaMKIV plays a critical role in the development and persistence of cocaine-induced behaviors, through mechanisms dissociated from acute effects on gene expression and CREB-dependent transcription. PMID: 19001277
    26. hnRNP L is an essential component of CaMKIV-regulated alternative splicing through CA repeats, with its phosphorylation likely playing a critical role. PMID: 19017650
    27. a group of RNA elements are responsive to PKA and CaMKIV from in vivo selection PMID: 19386606
    28. CaMKIV is a molecular link between Group I mGluRs and fragile X mental retardation protein in anterior cingulate cortex neurons PMID: 19436069
    29. analysis of regulation of calcium/calmodulin-dependent kinase IV by O-GlcNAc modification PMID: 19506079
    30. these data indicate that the B subunits alpha and delta are essential for the interaction of PP2A with CaMKIV. PMID: 19538941
    31. Data show that RA-induced repression of the CaMKIV signaling pathway may represent an early event in retinoid-dependent neuronal differentiation. PMID: 19633294

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
  • 组织特异性：
    Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue.
  • 数据库链接：

    HGNC: 1464

    OMIM: 114080

    KEGG: hsa:814

    STRING: 9606.ENSP00000282356

    UniGene: Hs.438801