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  4. Recombinant Human Calcium/calmodulin-dependent protein kinase type IV (CAMK4)

Recombinant Human Calcium/calmodulin-dependent protein kinase type IV (CAMK4)

  • 货号：
    CSB-YP614976HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP614976HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP614976HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP614976HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP614976HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Brain Ca(2+) calmodulin dependent protein kinase type 4; Brain Ca(2+) calmodulin dependent protein kinase type IV; Brain Ca++-calmodulin dependent protein kinase type IV; Calcium / calmodulin dependent protein kinase type 4 catalytic chain; Calcium / calmodulin dependent protein kinase type IV catalytic chain; Calcium/calmodulin dependent protein kinase IV ; Calcium/calmodulin dependent protein kinase type IV; Calcium/calmodulin-dependent protein kinase type IV; CAM kinase 4; CAM kinase GR; CAM kinase IV; CAM kinase-GR; CaMK 4; CAMK GR; CaMK IV; Camk4; CaMKGR ; IV; KCC4_HUMAN; MGC36771
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-473
  • 氨基酸序列
    MLKVTVPSCS ASSCSSVTAS AAPGTASLVP DYWIDGSNRD ALSDFFEVES ELGRGATSIV YRCKQKGTQK PYALKVLKKT VDKKIVRTEI GVLLRLSHPN IIKLKEIFET PTEISLVLEL VTGGELFDRI VEKGYYSERD AADAVKQILE AVAYLHENGI VHRDLKPENL LYATPAPDAP LKIADFGLSK IVEHQVLMKT VCGTPGYCAP EILRGCAYGP EVDMWSVGII TYILLCGFEP FYDERGDQFM FRRILNCEYY FISPWWDEVS LNAKDLVRKL IVLDPKKRLT TFQALQHPWV TGKAANFVHM DTAQKKLQEF NARRKLKAAV KAVVASSRLG SASSSHGSIQ ESHKASRDPS PIQDGNEDMK AIPEGEKIQG DGAQAAVKGA QAELMKVQAL EKVKGADINA EEAPKMVPKA VEDGIKVADL ELEEGLAEEK LKTVEEAAAP REGQGSSAVG FEVPQQDVIL PEY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.
  • 基因功能参考文献：
    1. vanillin binds strongly to the active site cavity of CAMKIV and stabilized by a large number of non-covalent interactions. PMID: 28744811
    2. Genotype and allele frequencies of CAMKIV gene SNPs differed significantly between alcohol dependence patients and control subjects. The results of the present study suggest that CAMKIV might be a candidate alcohol dependence gene. PMID: 28734942
    3. hTau accumulation impairs synapse and memory by CaN-mediated suppression of nuclear CaMKIV/CREB signaling. PMID: 27298345
    4. Within the pH range 5.0-11.5, CAMK4 maintained both its secondary and tertiary structures, along with its function, whereas significant aggregation was observed at acidic pH (2.0-4.5). PMID: 27032767
    5. A positive association was not observed between rs10491334 in the CAMK4 gene and longevity in a Chinese population. PMID: 27659345
    6. The T-allele of rs10491334 in CAMK4 was associated with hypertension in the Uygur group. PMID: 26909912
    7. Expression of CaMKIV inhibits autophosphorylation and activation of CaMKII, and elicits G0/G1cell cycle arrest,impairing cell proliferation. PMID: 25446257
    8. An imbalance of specific isoforms of CYFIP1, an FMRP interaction partner, and CAMK4, a transcriptional regulator of the FMRP gene, modulates risk for autism spectrum disorders. PMID: 24442360
    9. CaMK4-dependent activation of AKT/mTOR and CREM-alpha underlies autoimmunity-associated Th17 imbalance. PMID: 24667640
    10. CaMK4 regulates beta-cell proliferation and apoptosis in a CREB-dependent manner and CaMK4-induced IRS-2 expression is important in these processes PMID: 23049845
    11. study suggests that the mutations in CAMK4 may lead to abnormal semen parameters PMID: 22897820
    12. Phosphorylated Notch1-IC by CaMKIV increases Notch1-IC stability, which enhances osteoclast differentiation. PMID: 23103515
    13. Prolongevity genes are activated by CAMKIV, the levels of which are influenced by rs10491334, a single-nucleotide polymorphism associated with human longevity. PMID: 21612516
    14. The regulation of RORalpha activity by PKA as well as CaMK-IV provides a new link in the signalling network that regulates metabolic processes such as glycogen and lipid metabolism. PMID: 21514275
    15. These findings suggest that PLC/CAMK IV-NF-kappaB is involved in RAGE mediated signaling pathway in human endothelial cells. PMID: 20171262
    16. CaMKIV proteins were found in the nucleus of epithelial ovarian cancer tissue. CaMKIV expression was significantly associated with clinical stage (P<0.01), histological grade (P<0.01), and clinical outcome (P<0.01). PMID: 12065094
    17. sequestration of CaMKK may be the molecular mechanism by which catalytically inactive mutants of CaMKIV exert their "dominant-negative" functions within the cell PMID: 14701808
    18. the Ca(2+)/CaM binding-autoinhibitory domain of CaMKIV is required for association of the kinase with PP2A PMID: 15143065
    19. calcium/CaMKIV signaling pathway may play an important role in the excitation-mediated regulation of corticotropin releasing hormone synthesis PMID: 15591024
    20. the function of CaMK II is essential for PAF-induced macrophage priming, while CaMK IV is not specific for priming by PAF and appears to have a direct link in TLR4-mediated events PMID: 15665723
    21. CaMKIV is expressed in human sperm and may have a role in the regulation of human sperm motility PMID: 15840651
    22. Results identify calcium/calmodulin-dependent kinase IV as being responsible for the increased expression of CREM and the decreased production of interleukin-2 in systemic lupus erythematosus T cells. PMID: 15841182
    23. Transgenic CaMKIV plays a modulatory role in the nucleus accumbens in anxiety-like behavior of adult CaMKIV variant mice. PMID: 18053176
    24. CaMK-4 expression correlates positively with the ability to form long-term memory and implicates the decline of CaMKIV signaling mechanisms in age-related memory deficits. PMID: 18829949
    25. CaMKIV plays a critical role in the development and persistence of cocaine-induced behaviors, through mechanisms dissociated from acute effects on gene expression and CREB-dependent transcription. PMID: 19001277
    26. hnRNP L is an essential component of CaMKIV-regulated alternative splicing through CA repeats, with its phosphorylation likely playing a critical role. PMID: 19017650
    27. a group of RNA elements are responsive to PKA and CaMKIV from in vivo selection PMID: 19386606
    28. CaMKIV is a molecular link between Group I mGluRs and fragile X mental retardation protein in anterior cingulate cortex neurons PMID: 19436069
    29. analysis of regulation of calcium/calmodulin-dependent kinase IV by O-GlcNAc modification PMID: 19506079
    30. these data indicate that the B subunits alpha and delta are essential for the interaction of PP2A with CaMKIV. PMID: 19538941
    31. Data show that RA-induced repression of the CaMKIV signaling pathway may represent an early event in retinoid-dependent neuronal differentiation. PMID: 19633294

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
  • 组织特异性：
    Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue.
  • 数据库链接：

    HGNC: 1464

    OMIM: 114080

    KEGG: hsa:814

    STRING: 9606.ENSP00000282356

    UniGene: Hs.438801