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PSME3 Antibody

  • 货号：
    CSB-PA13674A0Rb
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA13674A0Rb at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA13674A0Rb at dilution of 1:100
    • Immunofluorescence staining of HepG2 cells with CSB-PA13674A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • IHC image of CSB-PA13674A0Rb diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PSME3 Polyclonal antibody
  • Uniprot No.：
    P61289
  • 基因名：
  • 别名：
    11S regulator complex gamma subunit antibody; 11S regulator complex subunit gamma antibody; Activator of multicatalytic protease subunit 3 antibody; Ki antibody; Ki antigen antibody; Ki nuclear autoantigen antibody; Ki, PA28 gamma antibody; PA28 gamma antibody; PA28g antibody; PA28gamma antibody; Proteasome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki) antibody; Proteasome (prosome, macropain) activator subunit 3 antibody; Proteasome activator 28 gamma antibody; Proteasome activator 28 subunit gamma antibody; Proteasome activator complex subunit 3 antibody; Proteasome activator subunit 3 antibody; PSME3 antibody; PSME3_HUMAN antibody; REG GAMMA antibody; REG-gamma antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Proteasome activator complex subunit 3 protein (2-252AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，PSME3 Antibody (CSB-PA13674A0Rb)，的标记方式是Non-conjugated。对于PSME3 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA13674B0Rb PSME3 Antibody, HRP conjugated ELISA
    FITC CSB-PA13674C0Rb PSME3 Antibody, FITC conjugated
    Biotin CSB-PA13674D0Rb PSME3 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition.
  • 基因功能参考文献：
    1. Knockdown of REG-GAMMA (REGgamma) may inhibit the proliferation and migration, and promote the apoptosis of plasma cell myeloma RPMI-8226 cells possibly by downregulating NF-kappa-B (NF-kappaB) signal pathway. PMID: 29020881
    2. data show that PIP30 deeply affects PA28gamma interactions with cellular proteins, including the 20S proteasome, demonstrating that it is an important regulator of PA28gamma in cells and thus a new player in the control of the multiple functions of the proteasome within the nucleus. PMID: 29934401
    3. the ubiquitin-independent REGgamma proteasome regulates energy homeostasis PMID: 27511885
    4. High expression of REGg seemed positively correlated with T-stage and lymph node metastasis in papillary thyroid carcinoma tissues. PMID: 29509725
    5. This study demonstrates that REGgamma is a central molecule in the development of melanoma by regulating Wnt/beta-catenin pathway. PMID: 28605165
    6. that Proteasome activator subunit 3 induces epithelial-mesenchymal transition with inducing the expression of CSC markers and influencing the tumor immune microenvironment in breast cancer PMID: 28529105
    7. PSME3 plays an oncogenic role in pancreatic cancer by inhibiting c-Myc degradation to promote glycolysis. PMID: 27756569
    8. Study demonstrated that the gene therapy with proteasome activator, PA28gamma can improve ubiquitin-proteasome system function as well as behavioral abnormalities in Huntington's disease model mice PMID: 26944602
    9. Surrogate Prognostic Biomarkers in OSCC: The Paradigm of PA28gamma Overexpression. PMID: 26425675
    10. PA28gamma in OSCC tumor tissues were significantly high expression than those in normal tissues. PMID: 26425691
    11. REGgamma acts in skin tumorigenesis mediating MAPK/p38 activation of the Wnt/beta-catenin pathway. PMID: 25908095
    12. Results show molecular cloning of a novel transcript variant encoding a truncated form of PA28G likely involved in cell cycle regulation and apoptosis. PMID: 25936920
    13. In a p53-dominated cellular context, pro-apoptotic signaling might be overcome by PA28gamma-mediated caspase inhibition. PMID: 26201457
    14. Our results suggest that the high expression of REGgamma might predict metastasis and poor prognosis in breast cancer. PMID: 25550823
    15. Increased PA28gamma sera levels were prognostic of disease activity in rheumatoid arthritis. PMID: 25482151
    16. Data suggest levels of gene expression of both PSME3 (proteasome activator subunit 3) and DUSP3 (dual specificity phosphatase 3) are associated with susceptibility to Staphylococcus aureus infection/sepsis in humans and in mouse disease model. PMID: 24901344
    17. Examination of EC and normal endometrium specimens confirmed the oncogenic role of REGgamma, in that REGgamma was more highly overexpressed in p53-positive specimens than in p53-negative specimens. PMID: 25697482
    18. PA28 gamma and p53 form a negative feedback loop that maintains the balance of p53 and PA28gamma in the cells. PMID: 24531141
    19. Our data indicate that miR-7-5p has a critical function through blocking REGgamma in breast cancer cells. PMID: 25511742
    20. REGgamma expression is positively correlated with ERalpha status and poor clinical prognosis in ERalpha positive breast cancer patients PMID: 25490392
    21. The results link Chk2 and REGgamma to the mechanism underlying the DBC1-dependent SIRT1 inhibition. PMID: 25361978
    22. PKA turnover by the REGgamma-proteasome modulates FoxO1 cellular activity and VEGF-induced angiogenesis. PMID: 24560667
    23. Expression of PA28gamma contributes to carcinogenesis and progression of colorectal cancer. PMID: 24113729
    24. The REGgamma-proteasome pathway is regulated differentially by p53/TGF-beta signaling and mutant p53 in cancer cells. PMID: 24157709
    25. the regulation of REGgamma assembly and activity, suggesting a potential venue for the intervention of the ubiquitin-independent REGgamma proteasome activity. PMID: 23612972
    26. PA28gamma acts as a co-repressor of HTLV-1 p30 to suppress virus replication and is required for the maintenance of viral latency. PMID: 23104922
    27. statistical analysisof laryngeal carcinomas revealed that there was a positive relationship between the level of REGgamma and the expression of p53 and p2; study suggests that REgamma overexpression can facilitate the growth of laryngeal cancer cells PMID: 22938444
    28. PA28gamma is an ATM target, being recruited to DNA damage sites where it is required for rapid accumulation of proteasomes, and the timely coordination of DNA double-strand break repair. PMID: 22134242
    29. REG-gamma associates with and modulates the abundance of nuclear activation-induced deaminase. PMID: 22042974
    30. A previously unrecognized mechanism regulating the activity of the proteasome activator REGgamma, is reported. PMID: 21445096
    31. High REGgamma expression is associated with breast cancer and its metastatic lymph nodes. PMID: 20467919
    32. HTLV-1 p30 interacts with ATM and REGgamma to increase viral spread by facilitating cell survival PMID: 21216954
    33. REGgamma regulates cellular distribution of p53 by facilitating its multiple monoubiquitylation and nuclear export. PMID: 21084564
    34. REGgamma-mediated p53 proteolysis contributes, as least in part, to the proviral function of REGgamma; the host REGgamma pathway is utilized and modified during CVB3 infection to promote efficient viral replication PMID: 20719955
    35. REGgamma is present in many tissues and the highest expression is in the testis. PMID: 20494959
    36. PA28gamma participates not only in the pathogenesis but also in the propagation of HCV by regulating the degradation of the core protein in both a ubiquitin-dependent and ubiquitin-independent manner PMID: 20683941
    37. Overexpression of the proteasome activator subunit PA28gamma recovered proteasome function in Huntington disease cells. It improved cell viability in mutant huntingtin-expressing striatal neurons exposed to pathological stressors. PMID: 17327906
    38. Data suggest REGgamma promoting tumor growth is a process involving multiple factor mechanisms. PMID: 19656465
    39. PA28gamma is an endogenous substrate for caspase-3 and -7 PMID: 11859414
    40. Ki antigen contains multiple epitopes recognized by autoimmune sera PMID: 12784391
    41. PA28 gamma novel regulator of Cajal body integrity in response to ultraviolet radiation. PMID: 17088425
    42. REGgamma proteasome activator is involved in the maintenance of chromosomal stability. PMID: 18235248
    43. PA28gamma, a proteasome activator that inhibits apoptosis and promotes cell cycle progression through unknown mechanisms, exerts an effect as a cofactor in the MDM2-p53 interaction. PMID: 18309296
    44. PA28gamma/REGgamma, which specifically binds to hepatitis c virus core protein, is required for the virulence of the core protein[review] PMID: 18321762
    45. mammalian proteasomes cannot degrade glutamine-expanded regions within pathogenic polyQ-expanded proteins, such as Huntingtin PMID: 18343811
    46. PA28gamma specifically binds to hepatitis C virus core protein and is involved in its degradation. PMID: 19091860
    47. these results underline a new role for REGgamma in the control and regulation of promyelocytic leukemia subnuclear structures. PMID: 19556897

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    PA28 family
  • 数据库链接：

    HGNC: 9570

    OMIM: 605129

    KEGG: hsa:10197

    STRING: 9606.ENSP00000293362

    UniGene: Hs.152978