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  4. Recombinant Human Proteasome activator complex subunit 3 (PSME3)

Recombinant Human Proteasome activator complex subunit 3 (PSME3)

  • 货号：
    CSB-YP018918HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018918HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018918HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018918HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    11S regulator complex gamma subunit; 11S regulator complex subunit gamma; Activator of multicatalytic protease subunit 3; Ki; Ki antigen; Ki nuclear autoantigen; Ki, PA28 gamma; PA28 gamma; PA28g; PA28gamma; Proteasome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki); Proteasome (prosome, macropain) activator subunit 3; Proteasome activator 28 gamma; Proteasome activator 28 subunit gamma; Proteasome activator complex subunit 3; Proteasome activator subunit 3; PSME3; PSME3_HUMAN; REG GAMMA; REG-gamma
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-254
  • 氨基酸序列
    ASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA ETLY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition.
  • 基因功能参考文献：
    1. Knockdown of REG-GAMMA (REGgamma) may inhibit the proliferation and migration, and promote the apoptosis of plasma cell myeloma RPMI-8226 cells possibly by downregulating NF-kappa-B (NF-kappaB) signal pathway. PMID: 29020881
    2. data show that PIP30 deeply affects PA28gamma interactions with cellular proteins, including the 20S proteasome, demonstrating that it is an important regulator of PA28gamma in cells and thus a new player in the control of the multiple functions of the proteasome within the nucleus. PMID: 29934401
    3. the ubiquitin-independent REGgamma proteasome regulates energy homeostasis PMID: 27511885
    4. High expression of REGg seemed positively correlated with T-stage and lymph node metastasis in papillary thyroid carcinoma tissues. PMID: 29509725
    5. This study demonstrates that REGgamma is a central molecule in the development of melanoma by regulating Wnt/beta-catenin pathway. PMID: 28605165
    6. that Proteasome activator subunit 3 induces epithelial-mesenchymal transition with inducing the expression of CSC markers and influencing the tumor immune microenvironment in breast cancer PMID: 28529105
    7. PSME3 plays an oncogenic role in pancreatic cancer by inhibiting c-Myc degradation to promote glycolysis. PMID: 27756569
    8. Study demonstrated that the gene therapy with proteasome activator, PA28gamma can improve ubiquitin-proteasome system function as well as behavioral abnormalities in Huntington's disease model mice PMID: 26944602
    9. Surrogate Prognostic Biomarkers in OSCC: The Paradigm of PA28gamma Overexpression. PMID: 26425675
    10. PA28gamma in OSCC tumor tissues were significantly high expression than those in normal tissues. PMID: 26425691
    11. REGgamma acts in skin tumorigenesis mediating MAPK/p38 activation of the Wnt/beta-catenin pathway. PMID: 25908095
    12. Results show molecular cloning of a novel transcript variant encoding a truncated form of PA28G likely involved in cell cycle regulation and apoptosis. PMID: 25936920
    13. In a p53-dominated cellular context, pro-apoptotic signaling might be overcome by PA28gamma-mediated caspase inhibition. PMID: 26201457
    14. Our results suggest that the high expression of REGgamma might predict metastasis and poor prognosis in breast cancer. PMID: 25550823
    15. Increased PA28gamma sera levels were prognostic of disease activity in rheumatoid arthritis. PMID: 25482151
    16. Data suggest levels of gene expression of both PSME3 (proteasome activator subunit 3) and DUSP3 (dual specificity phosphatase 3) are associated with susceptibility to Staphylococcus aureus infection/sepsis in humans and in mouse disease model. PMID: 24901344
    17. Examination of EC and normal endometrium specimens confirmed the oncogenic role of REGgamma, in that REGgamma was more highly overexpressed in p53-positive specimens than in p53-negative specimens. PMID: 25697482
    18. PA28 gamma and p53 form a negative feedback loop that maintains the balance of p53 and PA28gamma in the cells. PMID: 24531141
    19. Our data indicate that miR-7-5p has a critical function through blocking REGgamma in breast cancer cells. PMID: 25511742
    20. REGgamma expression is positively correlated with ERalpha status and poor clinical prognosis in ERalpha positive breast cancer patients PMID: 25490392
    21. The results link Chk2 and REGgamma to the mechanism underlying the DBC1-dependent SIRT1 inhibition. PMID: 25361978
    22. PKA turnover by the REGgamma-proteasome modulates FoxO1 cellular activity and VEGF-induced angiogenesis. PMID: 24560667
    23. Expression of PA28gamma contributes to carcinogenesis and progression of colorectal cancer. PMID: 24113729
    24. The REGgamma-proteasome pathway is regulated differentially by p53/TGF-beta signaling and mutant p53 in cancer cells. PMID: 24157709
    25. the regulation of REGgamma assembly and activity, suggesting a potential venue for the intervention of the ubiquitin-independent REGgamma proteasome activity. PMID: 23612972
    26. PA28gamma acts as a co-repressor of HTLV-1 p30 to suppress virus replication and is required for the maintenance of viral latency. PMID: 23104922
    27. statistical analysisof laryngeal carcinomas revealed that there was a positive relationship between the level of REGgamma and the expression of p53 and p2; study suggests that REgamma overexpression can facilitate the growth of laryngeal cancer cells PMID: 22938444
    28. PA28gamma is an ATM target, being recruited to DNA damage sites where it is required for rapid accumulation of proteasomes, and the timely coordination of DNA double-strand break repair. PMID: 22134242
    29. REG-gamma associates with and modulates the abundance of nuclear activation-induced deaminase. PMID: 22042974
    30. A previously unrecognized mechanism regulating the activity of the proteasome activator REGgamma, is reported. PMID: 21445096
    31. High REGgamma expression is associated with breast cancer and its metastatic lymph nodes. PMID: 20467919
    32. HTLV-1 p30 interacts with ATM and REGgamma to increase viral spread by facilitating cell survival PMID: 21216954
    33. REGgamma regulates cellular distribution of p53 by facilitating its multiple monoubiquitylation and nuclear export. PMID: 21084564
    34. REGgamma-mediated p53 proteolysis contributes, as least in part, to the proviral function of REGgamma; the host REGgamma pathway is utilized and modified during CVB3 infection to promote efficient viral replication PMID: 20719955
    35. REGgamma is present in many tissues and the highest expression is in the testis. PMID: 20494959
    36. PA28gamma participates not only in the pathogenesis but also in the propagation of HCV by regulating the degradation of the core protein in both a ubiquitin-dependent and ubiquitin-independent manner PMID: 20683941
    37. Overexpression of the proteasome activator subunit PA28gamma recovered proteasome function in Huntington disease cells. It improved cell viability in mutant huntingtin-expressing striatal neurons exposed to pathological stressors. PMID: 17327906
    38. Data suggest REGgamma promoting tumor growth is a process involving multiple factor mechanisms. PMID: 19656465
    39. PA28gamma is an endogenous substrate for caspase-3 and -7 PMID: 11859414
    40. Ki antigen contains multiple epitopes recognized by autoimmune sera PMID: 12784391
    41. PA28 gamma novel regulator of Cajal body integrity in response to ultraviolet radiation. PMID: 17088425
    42. REGgamma proteasome activator is involved in the maintenance of chromosomal stability. PMID: 18235248
    43. PA28gamma, a proteasome activator that inhibits apoptosis and promotes cell cycle progression through unknown mechanisms, exerts an effect as a cofactor in the MDM2-p53 interaction. PMID: 18309296
    44. PA28gamma/REGgamma, which specifically binds to hepatitis c virus core protein, is required for the virulence of the core protein[review] PMID: 18321762
    45. mammalian proteasomes cannot degrade glutamine-expanded regions within pathogenic polyQ-expanded proteins, such as Huntingtin PMID: 18343811
    46. PA28gamma specifically binds to hepatitis C virus core protein and is involved in its degradation. PMID: 19091860
    47. these results underline a new role for REGgamma in the control and regulation of promyelocytic leukemia subnuclear structures. PMID: 19556897

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    PA28 family
  • 数据库链接：

    HGNC: 9570

    OMIM: 605129

    KEGG: hsa:10197

    STRING: 9606.ENSP00000293362

    UniGene: Hs.152978