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PIK3R6 Antibody

  • 货号：
    CSB-PA719469LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate
      All lanes: PIK3R6 antibody at 3.4µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 85 kDa
      Observed band size: 85 kDa
    • IHC image of CSB-PA719469LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA719469LA01HU diluted at 1:400 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA719469LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PIK3R6 Polyclonal antibody
  • Uniprot No.：
    Q5UE93
  • 基因名：
  • 别名：
    C17orf38 antibody; Chromosome 17 open reading frame 38 antibody; HsT41028 antibody; p84 PI3K adapter protein antibody; p84 PIKAP antibody; p87 phosphoinositide 3-kinase gamma (PI3Kg) adapter protein antibody; p87 PI3K adapter protein antibody; p87PIKAP antibody; Phosphatidylinositol 3-kinase; regulatory subunit 6 antibody; Phosphoinositide 3-kinase gamma adapter protein of 87 kDa antibody; Phosphoinositide 3-kinase regulatory subunit 6 antibody; PI3Kgamma adapter protein of 87 kDa antibody; PI3R6_HUMAN antibody; PIK3R6 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Phosphoinositide 3-kinase regulatory subunit 6 protein (566-667AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，PIK3R6 Antibody (CSB-PA719469LA01HU)，的标记方式是Non-conjugated。对于PIK3R6 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA719469LB01HU PIK3R6 Antibody, HRP conjugated ELISA
    FITC CSB-PA719469LC01HU PIK3R6 Antibody, FITC conjugated
    Biotin CSB-PA719469LD01HU PIK3R6 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Regulatory subunit of the PI3K gamma complex. Acts as an adapter to drive activation of PIK3CG by beta-gamma G protein dimers. The PIK3CG:PIK3R6 heterodimer is much less sensitive to beta-gamma G protein dimers than PIK3CG:PIK3R5 and its membrane recruitment and beta-gamma G protein dimer-dependent activation requires HRAS bound to PIK3CG. Recruits of the PI3K gamma complex to a PDE3B:RAPGEF3 signaling complex involved in angiogenesis; signaling seems to involve RRAS.
  • 基因功能参考文献：
    1. Our findings suggest that p84 binding to p110gamma may represent a novel negative feedback signal that terminates PI3Kgamma activity. PMID: 25753393
    2. expression and activities of PI3Kgamma are modified differently by p87 and p101 in vitro and in living cells, arguing for specific regulatory roles of the non-catalytic subunits in the differentiation of PI3Kgamma signaling pathways. PMID: 24014027
    3. knockdown of p84 in MDA-MB-231 cells enhanced Akt phosphorylation and lung colonization PMID: 21996737
    4. p87PIKAP is a novel regulatory subunit of phosphoinositide 3-kinase gamma that is highly expressed in heart and interacts with PDE3B [p87PIKAP] PMID: 16476736
    5. ablation of PI3Kgamma reduced SDF1alpha-induced integrin activation in human EPCs and in murine Lin(-) BM-derived progenitor cells PMID: 18323525
    6. RACK1 regulates directional cell migration by acting on G betagamma at the interface with its effectors PLC beta and PI3K gamma PMID: 18596232

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  • 亚细胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein.
  • 数据库链接：

    HGNC: 27101

    OMIM: 611462

    KEGG: hsa:146850

    UniGene: Hs.255809