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ONECUT1 Antibody

  • 货号：
    CSB-PA880058LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Jurkat whole cell lysate, Rat brain tissue
      All lanes: ONECUT1 antibody at 7.6μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 52 kDa
      Observed band size: 52 kDa
    • IHC image of CSB-PA880058LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ONECUT1 Polyclonal antibody
  • Uniprot No.：
    Q9UBC0
  • 基因名：
  • 别名：
    hepatocyte nuclear factor 6 alpha antibody; Hepatocyte nuclear factor 6 antibody; HNF 6 antibody; HNF-6 antibody; HNF6_HUMAN antibody; HNF6A antibody; One cut domain family member 1 antibody; One cut homeobox 1 antibody; Onecut1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Rat
  • 免疫原：
    Recombinant Human Hepatocyte nuclear factor 6 protein (192-292AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ONECUT1 Antibody (CSB-PA880058LA01HU)，的标记方式是Non-conjugated。对于ONECUT1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA880058LB01HU ONECUT1 Antibody, HRP conjugated ELISA
    FITC CSB-PA880058LC01HU ONECUT1 Antibody, FITC conjugated
    Biotin CSB-PA880058LD01HU ONECUT1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
  • 基因功能参考文献：
    1. Reduced HNF6 expression is associated with metastasis of cholangiocarcinoma. PMID: 26825606
    2. HNF6 is a novel host factor that can restrict hepatitis B virus replication via both transcriptional and posttranscriptional mechanisms. PMID: 25653429
    3. The negative correlation between HNF6 expression and pancreatic cancer progression suggests that HNF6 maintains pancreatic epithelial homeostasis, and that its loss contributes to the progression from intraepithelial neoplasms to ductal adenocarcinoma. PMID: 24638272
    4. HNF6 plays an important role in the gene regulation of CYP3A4 during development from the fetal period to the postnatal period. PMID: 23291635
    5. HNF6 inhibits EMT, cell migration, and invasive growth through a mechanism involving the transcriptional activation of p53. PMID: 24022481
    6. Mutations at the -5/-6 site of the F9 promotor account for the majority of hemophilia B Leyden cases and disrupt the binding of ONECUT1 and ONECUT2. PMID: 23472758
    7. HNF6 and Sox9 are new biomarkers of pancreatic acinar-to-ductal metaplasia and constitute candidate targets for preventive treatment in cases when acinar-to-ductal metaplasia may lead to cancer. PMID: 22271799
    8. Hepatocyte nuclear factor 6 is one of the liver-enriched transcription factors, it utilizes the bipartite onecut-homeodomain sequence to localize to the nuclear compartment and bind specific DNA sequences of numerous target gene promoters PMID: 21893194
    9. HNF6 has a role as a coactivator protein to potentiate the transcriptional activity of another liver factor, FoxA2 PMID: 12509444
    10. These findings define a precursor cellular stage of the embryonic pancreas and place Hnf1beta in a genetic hierarchy that regulates the generation of pancreatic endocrine cells. PMID: 14570708
    11. identifed systematically the genes occupied by the transcriptional regulators HNF1alpha, HNF4alpha, and HNF6, together with RNA polymerase II in human liver and pancreatic islets PMID: 14988562
    12. HNF6 protein stability is controlled by CBP acetylation PMID: 15304484
    13. A comparision of gene expression patterns induced by hepatic nuclear factors, HNF6, HNF4alpha and HNF1beta, in a pancreatic beta-cell line. PMID: 15520459
    14. ONECUT1 expression is lost in pancreatic cancer cells, suggesting a tumor suppressor function in this malignancy. PMID: 18097590
    15. Results suggest that HNF-6 is a novel target of small heterodimer partner in the regulation of gluconeogenesis. PMID: 18459945

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    CUT homeobox family
  • 组织特异性：
    Highly expressed in liver; lower expression in testis and skin.
  • 数据库链接：

    HGNC: 8138

    OMIM: 604164

    KEGG: hsa:3175

    STRING: 9606.ENSP00000302630

    UniGene: Hs.555318