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  4. NCK2 Antibody

NCK2 Antibody

  • 货号：
    CSB-PA126963
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA126963(NCK2 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: Mouse thymus tissue, Primary antibody: CSB-PA126963(NCK2 Antibody) at dilution 1/900, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds
  • 其他：

产品详情

  • Uniprot No.：
    O43639
  • 基因名：
  • 别名：
    Cytoplasmic protein NCK2 antibody; GRB4 antibody; Growth factor receptor bound protein 4 antibody; NCK adaptor protein 2 antibody; Nck-2 antibody; NCK2 antibody; NCK2_HUMAN antibody; NCKbeta antibody; Non-catalytic region of tyrosine kinase adaptor protein 2 antibody; Noncatalytic region of tyrosine kinase beta antibody; SH2/SH3 adaptor protein NCK-beta antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthetic peptide of Human NCK2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    WB 1:500-1:2000
    IHC 1:25-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
  • 基因功能参考文献：
    1. This study provides evidence of a possible role of NCK2 as biomarker of ovarian cancer progression. PMID: 29218693
    2. There was no difference in the Nck2 mRNA expression between the SLE patients and the healthy subjects. PMID: 29911835
    3. Nck1 and Nck2 Interact with WTIP. Nck1/2 integrates nephrin with the Hippo kinase cascade through association with the adaptor protein WTIP. PMID: 27033705
    4. Data suggest that PINCH1 and Nck2 critically participate in the regulation of cellular radiosensitivity and EGFR function and downstream signaling in a cellular model of human squamous cell carcinoma. PMID: 26004008
    5. Tir-Intimin interaction recruits the Nck adaptor to a Tir tyrosine phosphorylated residue where it activates neural Wiskott-Aldrich syndrome protein (N-WASP). PMID: 25482634
    6. Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics. PMID: 24287595
    7. NCK2 is involved in the susceptibility to opiates addiction. PMID: 23533358
    8. The hNck2 SH3 domain also exhibited pH dependent monomer-dimer transition. PMID: 23524290
    9. Data show that both HK2 and NCK2 are expressed in the retinal ganglion cell layer. PMID: 23349798
    10. Nck2 effectively influences human melanoma phenotype progression. PMID: 21992144
    11. p21-Activated kinase 3 (PAK3) protein regulates synaptic transmission through its interaction with the Nck2/Grb4 protein adaptor. PMID: 21949127
    12. Nck2 is an adaptor protein composed of 3N-SH3 domains followed by a unique Cterminal SH2 domain. It interacts with PINCH in integrin signal transduction, cell migratio and survival. Review. PMID: 21880263
    13. biophysical analysis of Nck2 SH3 domain PMID: 19956763
    14. Nck-2 interacts with focal adhesion kinase and modulates cell motility. Using a mutational strategy, the formation of the Nck-2-FAK complex is mediated by interactions involving multiple SH2 and SH3 domains of Nck-2. PMID: 11950595
    15. Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. PMID: 12110186
    16. analysis of binding between the Tyr-phosphorylated human ephrinB2 and Nck2 SH2 domain PMID: 15764601
    17. Molecular model of the first SRC homology 3 domain of Nck2. PMID: 16604428
    18. Nck1 (Nckalpha) and Nck2 (Nckbeta and Grb4): binding specificities of both SH2 domains are essentially indistinguishable PMID: 16636066
    19. determined the NMR structures and dynamic properties of the hNck2 SH3 domains and to define their ligand binding preferences with nine proline-rich peptides derived from Wire, CAP-1, CAP-2, Prk, Wrch1, Wrch2, and Nogo PMID: 16752908
    20. Data show that Nck (isoforms 1 and 2) as a component of the CReP/PP1c holophosphatase complex contributes to maintain eIF2alpha in a hypophosphorylated state, and modulates translation and eIF2alpha signaling in response to ER stress. PMID: 16835242
    21. Demonstrate connection between septins/SOCS7/NCK signaling and the DNA damage response. PMID: 17803907
    22. the adaptors Nck and ShcA influenced adherence of S. Typhimurium to non-phagocytic cells. PMID: 17906149
    23. Data show that Nck forms a complex with an atypical PxxDY motif of the CD3epsilon tail, which encompasses Tyr166 within the activation motif and a T-cell receptor endocytosis signal. PMID: 18555270
    24. Results point to NCK2 as a disease candidate gene and further supports the GLC1B locus as an important genomic region that is associated with the genetic predisposition to glaucoma. PMID: 18723748
    25. independent roles and mechanisms of action of Nckalpha and Nckbeta in dermal fibroblast migration, which is critical for wound healing. PMID: 19242519

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  • 亚细胞定位：
    Cytoplasm. Endoplasmic reticulum.
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 7665

    OMIM: 604930

    KEGG: hsa:8440

    STRING: 9606.ENSP00000233154

    UniGene: Hs.529244