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  4. Recombinant Human Cytoplasmic domain protein NCK2 (NCK2)

Recombinant Human Cytoplasmic domain protein NCK2 (NCK2)

  • 货号：
    CSB-EP015531HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cytoplasmic protein NCK2; GRB4; Growth factor receptor bound protein 4; NCK adaptor protein 2; Nck-2; NCK2; NCK2_HUMAN; NCKbeta; Non-catalytic region of tyrosine kinase adaptor protein 2; Noncatalytic region of tyrosine kinase beta; SH2/SH3 adaptor protein NCK-beta
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    58.9kDa
  • 表达区域：
    1-380aa
  • 氨基酸序列
    MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
  • 基因功能参考文献：
    1. This study provides evidence of a possible role of NCK2 as biomarker of ovarian cancer progression. PMID: 29218693
    2. There was no difference in the Nck2 mRNA expression between the SLE patients and the healthy subjects. PMID: 29911835
    3. Nck1 and Nck2 Interact with WTIP. Nck1/2 integrates nephrin with the Hippo kinase cascade through association with the adaptor protein WTIP. PMID: 27033705
    4. Data suggest that PINCH1 and Nck2 critically participate in the regulation of cellular radiosensitivity and EGFR function and downstream signaling in a cellular model of human squamous cell carcinoma. PMID: 26004008
    5. Tir-Intimin interaction recruits the Nck adaptor to a Tir tyrosine phosphorylated residue where it activates neural Wiskott-Aldrich syndrome protein (N-WASP). PMID: 25482634
    6. Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics. PMID: 24287595
    7. NCK2 is involved in the susceptibility to opiates addiction. PMID: 23533358
    8. The hNck2 SH3 domain also exhibited pH dependent monomer-dimer transition. PMID: 23524290
    9. Data show that both HK2 and NCK2 are expressed in the retinal ganglion cell layer. PMID: 23349798
    10. Nck2 effectively influences human melanoma phenotype progression. PMID: 21992144
    11. p21-Activated kinase 3 (PAK3) protein regulates synaptic transmission through its interaction with the Nck2/Grb4 protein adaptor. PMID: 21949127
    12. Nck2 is an adaptor protein composed of 3N-SH3 domains followed by a unique Cterminal SH2 domain. It interacts with PINCH in integrin signal transduction, cell migratio and survival. Review. PMID: 21880263
    13. biophysical analysis of Nck2 SH3 domain PMID: 19956763
    14. Nck-2 interacts with focal adhesion kinase and modulates cell motility. Using a mutational strategy, the formation of the Nck-2-FAK complex is mediated by interactions involving multiple SH2 and SH3 domains of Nck-2. PMID: 11950595
    15. Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. PMID: 12110186
    16. analysis of binding between the Tyr-phosphorylated human ephrinB2 and Nck2 SH2 domain PMID: 15764601
    17. Molecular model of the first SRC homology 3 domain of Nck2. PMID: 16604428
    18. Nck1 (Nckalpha) and Nck2 (Nckbeta and Grb4): binding specificities of both SH2 domains are essentially indistinguishable PMID: 16636066
    19. determined the NMR structures and dynamic properties of the hNck2 SH3 domains and to define their ligand binding preferences with nine proline-rich peptides derived from Wire, CAP-1, CAP-2, Prk, Wrch1, Wrch2, and Nogo PMID: 16752908
    20. Data show that Nck (isoforms 1 and 2) as a component of the CReP/PP1c holophosphatase complex contributes to maintain eIF2alpha in a hypophosphorylated state, and modulates translation and eIF2alpha signaling in response to ER stress. PMID: 16835242
    21. Demonstrate connection between septins/SOCS7/NCK signaling and the DNA damage response. PMID: 17803907
    22. the adaptors Nck and ShcA influenced adherence of S. Typhimurium to non-phagocytic cells. PMID: 17906149
    23. Data show that Nck forms a complex with an atypical PxxDY motif of the CD3epsilon tail, which encompasses Tyr166 within the activation motif and a T-cell receptor endocytosis signal. PMID: 18555270
    24. Results point to NCK2 as a disease candidate gene and further supports the GLC1B locus as an important genomic region that is associated with the genetic predisposition to glaucoma. PMID: 18723748
    25. independent roles and mechanisms of action of Nckalpha and Nckbeta in dermal fibroblast migration, which is critical for wound healing. PMID: 19242519

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  • 亚细胞定位：
    Cytoplasm. Endoplasmic reticulum.
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 7665

    OMIM: 604930

    KEGG: hsa:8440

    STRING: 9606.ENSP00000233154

    UniGene: Hs.529244