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Hspa5 Antibody

  • 货号：
    CSB-PA010827YA01MO
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Rat liver tissue, Rat brain tissue, Mouse brain tissue
      All lanes: Hspa5 antibody at 3μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 73 kDa
      Observed band size: 73 kDa
    • Immunofluorescent analysis of HepG2 cells using CSB-PA010827YA01MO at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Mus musculus (Mouse) Hspa5 Polyclonal antibody
  • Uniprot No.：
    P20029
  • 基因名：
  • 别名：
    Hspa5 antibody; Grp78 antibody; Endoplasmic reticulum chaperone BiP antibody; EC 3.6.4.10 antibody; 78 kDa glucose-regulated protein antibody; GRP-78 antibody; Binding-immunoglobulin protein antibody; BiP antibody; Heat shock protein 70 family protein 5 antibody; HSP70 family protein 5 antibody; Heat shock protein family A member 5 antibody; Immunoglobulin heavy chain-binding protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Mouse, Rat
  • 免疫原：
    Recombinant Mouse Endoplasmic reticulum chaperone BiP protein (20-655AA)
  • 免疫原种属：
    Mus musculus (Mouse)
  • 标记方式：
    Non-conjugated

    本页面中的产品，Hspa5 Antibody (CSB-PA010827YA01MO)，的标记方式是Non-conjugated。对于Hspa5 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA010827YB01MO Hspa5 Antibody, HRP conjugated ELISA
    FITC CSB-PA010827YC01MO Hspa5 Antibody, FITC conjugated
    Biotin CSB-PA010827YD01MO Hspa5 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:2000-1:10000
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1. Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation.
  • 基因功能参考文献：
    1. GRP78 regulates insulin resistance and macrophage polarization with selective IL-6 secretion in diet-induced obesity. PMID: 29242277
    2. These results uncover a novel role of GRP78 in reducing prion pathogenesis, suggesting that modulating its levels/activity may offer a novel opportunity for designing therapeutic approaches for these diseases. PMID: 28333162
    3. Endoplasmic reticulum stress leads to de novo biosynthesis of non-trimethylated GRP78, whereas homeostatic, METTL21A-dependent lysine 585-trimethylated GRP78 is reduced. PMID: 28912266
    4. GRP78 haploinsufficiency for up to 2 years of age has no major deleterious effect in rodents of different genetic background. PMID: 28145503
    5. GRP78 promotes cigarette smoke-induced inflammatory response and mucus hyperproduction in airway epithelial cells, likely through upregulation of necroptosis and subsequent activation of NF-kappaB and AP-1 pathways. PMID: 29445274
    6. siRNA of Grp78 disturbed the fusion of mouse palate cultured in vitro, suggesting a role in the palate development during embryogenesis.. PMID: 29233047
    7. prolonged endoplasmic reticulum stress promotes apoptosis via a p53-dependent inhibition of BiP expression PMID: 28622297
    8. role in acinar-to-ductal metaplasia and pancreatic ductal adenocarcinoma PMID: 28461470
    9. analysis of the effects of triptolide on cell proliferation, cell cycle and the expression of GRP78 in nasopharyngeal carcinoma PMID: 27391061
    10. candidate genes that modulate Hspa5 expression in the retina, were examined. PMID: 27881906
    11. These results indicate that GRP78, but not nutritional status, is a potent up-regulator of hepatic PTC-mRNA levels during induction of ER stress in vivo. PMID: 28383761
    12. Data suggest that activation of GRP78/Ire1/Xbp1 pathway of ER stress-unfolded protein response is involved in mouse decidualization. PMID: 27283502
    13. These results demonstrate a key role for GRP78 in alveolar epithelial cell survival. PMID: 26816051
    14. These results indicate that GRP78, an endoplasmic reticulum chaperon of the HSP70 family, is a novel host factor involved at multiple steps of the Japanese encephalitis virus life cycle and could be a potential therapeutic target. PMID: 28053106
    15. Genetic or pharmacologic inhibition of the HSPA5-GPX4 pathway enhanced gemcitabine sensitivity by disinhibiting ferroptosis in vitro and in both subcutaneous and orthotopic animal models of PDAC. PMID: 28130223
    16. Endoplasmic reticulum stress gene GRP78 is involved in signaling pathway during hepatitis B virus-mediated hepatocarcinogenesis. PMID: 26567840
    17. data show that Med inhibits ER stress-induced apoptosis and promotes osteoblast cell survival by targeting GRP78. PMID: 27316719
    18. These results suggested the important roles of endoplasmic reticulum-related chaperons, Bip and SIL1, in Alzheimer's disease-like tau hyperphosphorylation. PMID: 25575678
    19. We show that chronic VPA treatment did not modify the ATXN3 inclusion load and astrogliosis in affected brain regions However, VPA chronic treatment was able to increase GRP78 protein levels at 30 weeks of age, one of its known neuroprotective effects PMID: 26505994
    20. these findings reveal a novel critical role of GRP78 in regulating ER stress-mediated apoptosis in cartilage development and the molecular mechanisms involved. PMID: 26370957
    21. ISM is a novel vascular permeability inducer that functions through cell-surface GRP78-mediated Src activation as well as induction of apoptosis PMID: 25952901
    22. BIP increased surface CD19 molecule expression intensity and IL-10(+), PD-L1(hi) and FasL(hi) B cells induced by BIP share the CD19(hi) phenotype PMID: 26655428
    23. These studies demonstrate that BiP is critical for myelinating cell survival and contributes to the protective response of oligodendrocyte against inflammatory demyelination. PMID: 26631473
    24. These studies imply GRP78, but not GRP94, is required for mammary gland development. PMID: 24953136
    25. A highly specific monoclonal antibody against GRP78 suppressed AKT activation and increased apoptosis in the cPten(f/f) tumors. PMID: 25684138
    26. AdGRP78 reduced expression of lipogenic genes and plasma triglycerides in the db/db strain. Both G5 and G8 protein levels increased as did total biliary cholesterol PMID: 26365598
    27. Heterozygous mutant BiP mice revealed motor disabilities in aging. PMID: 25405877
    28. findings underscore the specific and prominent role of SKIP and GRP78 in the regulation of insulin-dependent PI 3-kinase signaling in skeletal muscle PMID: 26376412
    29. STZ injection i.p. rapidly induced up-regulation of the ER stress marker, the prosurvival chaperone glucose-regulated protein 78. With the development of diabetes, the expression of GRP78 decreases. PMID: 25529350
    30. Downregulation of GRP78 abolishes the activity of exogenous Syn, indicating that it is the primary target of Syn. PMID: 25124556
    31. high glucose induced astrocytic activation in both in vivo and in vitro diabetic models, in which modulation of GRP78 would be an important event in this activation PMID: 24056253
    32. This study showed that that inflammation leads to citrullination of GRP78 in beta-cells; opening new avenues for biomarker development and therapeutic intervention. PMID: 25204978
    33. Systemic gene transfer of binding immunoglobulin protein (BiP) prevents disease progression in murine collagen-induced arthritis. PMID: 25228326
    34. Data suggest that the balance between Cripto and Grp78 expression levels might be crucial in cancer development and may account for the increased tumorigenesis in Cripto heterozygous mice. PMID: 24805056
    35. Deficiency of the BiP cochaperone ERdj4 causes constitutive endoplasmic reticulum stress and metabolic defects. PMID: 24336520
    36. GRP78 might play an important role during the process of mouse embryo implantation, and GRP78 expression was mainly regulated by active blastocysts and maternal oestrogen. PMID: 24242779
    37. there is a causal link between a humoral response to GRP78 and the progression of cancer in a murine melanoma model PMID: 21164368
    38. GRP78 is a novel regulator for PTEN-loss-mediated liver injury and cancer progression. PMID: 24141775
    39. Matrin 3 interacts specifically with the heat shock proteins glucose-regulated protein 78, GRP75 and glutathione S-transferase pi isoform 2. PMID: 24491357
    40. Reg2 induces glucose-regulated peptide 78 (GRP78) expression via the Akt-mTORC1 axis. PMID: 24801175
    41. downregulated miR-181b induces neuroprotection against ischemic injury through negatively regulating HSPA5 and UCHL1 protein levels PMID: 23900885
    42. the interaction of amelogenin with Grp78/Bip contributed to cell proliferation, rather than correlate with the osteogenic differentiation PMID: 24167599
    43. IRE1alpha dissociates from BiP and inhibits ER stress-mediated apoptosis in the process of cartilage development. PMID: 23816533
    44. Data indicate that GRP78 is expressed in metastatic mammary tumors. PMID: 23470966
    45. Data suggest that transgenic mice that overproduce GRP78 in pancreatic beta cells are leaner than non-transgenic littermates and are protected against development of glucose intolerance, insulin resistance, and endoplasmic reticulum stress in islets. PMID: 23475366
    46. Knockdown of GRP78/BiP increased the accumulation of AR aggregates and significantly higher levels of caspase-3 activity and cell apoptosis. PMID: 23618905
    47. BiP was particularly observed in the pathological vasculature and retinal microvascular endothelial cells, and the increase of BiP expression was correlated with retinal neovascularization. PMID: 23544152
    48. These findings demonstrate a sensitive requirement for the endoplasmic reticulum chaperone BiP/GRP78 during axon outgrowth and pathfinding in the developing mammalian brain. PMID: 22821687
    49. GRP78 is required for adipocyte differentiation, glucose homeostasis, and balanced secretion of adipokines. PMID: 23180827
    50. NPGPx is essential for releasing excessive ER stress by enhancing GRP78 chaperone activity to maintain physiological homeostasis. PMID: 23123197

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  • 亚细胞定位：
    Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Cell surface.
  • 蛋白家族：
    Heat shock protein 70 family
  • 数据库链接：

    KEGG: mmu:14828

    STRING: 10090.ENSMUSP00000028222

    UniGene: Mm.330160