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AOX1 Antibody

  • 货号：
    CSB-PA000885
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of HeLa cells using AOX1 Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    Q06278
  • 基因名：
  • 别名：
    AOX1 antibody; AOAldehyde oxidase antibody; EC 1.2.3.1 antibody; Aldehyde oxidase 1 antibody; Azaheterocycle hydroxylase antibody; EC 1.17.3.- antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Rat
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human AOX1.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:40000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Oxidase with broad substrate specificity, oxidizing aromatic azaheterocycles, such as N1-methylnicotinamide, N-methylphthalazinium and phthalazine, as well as aldehydes, such as benzaldehyde, retinal, pyridoxal, and vanillin. Plays a key role in the metabolism of xenobiotics and drugs containing aromatic azaheterocyclic substituents. Participates in the bioactivation of prodrugs such as famciclovir, catalyzing the oxidation step from 6-deoxypenciclovir to penciclovir, which is a potent antiviral agent. Is probably involved in the regulation of reactive oxygen species homeostasis. May be a prominent source of superoxide generation via the one-electron reduction of molecular oxygen. Also may catalyze nitric oxide (NO) production via the reduction of nitrite to NO with NADH or aldehyde as electron donor. May play a role in adipogenesis.
  • 基因功能参考文献：
    1. AOX1 SNPs exist in which the base-pair exchange of the SNP leads to loss of enzyme activity. PMID: 26842593
    2. ten novel single nucleotide polymorphisms resulting in amino acid exchanges in proximity to the FAD site of hAOX1, were characterized. PMID: 28750088
    3. Data suggest that substrate specificity of AOX1 includes GDC-0834, an inhibitor of Bruton's tyrosine kinase with potential non-steroidal anti-inflammatory agent activity; in liver cytosol, hydrolysis of GDC-0834 is primarily due to AOX1. PMID: 25845827
    4. Methotrexate is poorly metabolized by human AOX1, in contrast to rabbit. PMID: 26032640
    5. These results supported the possibility that XO/XD and AO can contribute to nitric oxide generation. PMID: 25537183
    6. Several donors have a normal AOX1 protein level. PMID: 23857892
    7. High AOX1 methylation was associated with biochemical recurrence in prostate cancer. PMID: 23918943
    8. The physiological role of AOX1 remains unclear, but it is known that thiopurines belong to its substrates; AOX1 contributes to azathioprine catabolism. PMID: 22495427
    9. hydralazine, at a concentration of 25 to 50 muM, can be used in human hepatocyte incubations to estimate the contribution of aldehyde oxidase to the hepatic clearance of drugs and other compounds. PMID: 22522748
    10. Human population is characterized by the presence of functionally inactive hAOX1 allelic variants as well as variants encoding enzymes with different catalytic activities. PMID: 22279051
    11. characterization of AO activity in cryopreserved human hepatocytes: enzyme stability; substrate specificity; kinetics; inhibition by hydralazine PMID: 22031625
    12. Expressed AOX1 in Escherichia coli and purified kinetically active protein. PMID: 19741035

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Xanthine dehydrogenase family
  • 组织特异性：
    Abundant in liver, expressed in adipose tissue and at lower levels in lung, skeletal muscle, pancreas. In contrast to mice, no significant gender difference in AOX1 expression level (at protein level).
  • 数据库链接：

    HGNC: 553

    OMIM: 602841

    KEGG: hsa:316

    STRING: 9606.ENSP00000363832

    UniGene: Hs.406238