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  4. Recombinant Human Aldehyde oxidase (AOX1), partial

Recombinant Human Aldehyde oxidase (AOX1), partial

In Stock
  • 货号：
    CSB-EP001858HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    AOX1; AOAldehyde oxidase; EC 1.2.3.1; Aldehyde oxidase 1; Azaheterocycle hydroxylase; EC 1.17.3.-
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    24.6kDa
  • 表达区域：
    236-421aa
  • 氨基酸序列
    FGSERMMWFSPVTLKELLEFKFKYPQAPVIMGNTSVGPEVKFKGVFHPVIISPDRIEELSVVNHAYNGLTLGAGLSLAQVKDILADVVQKLPEEKTQMYHALLKHLGTLAGSQIRNMASLGGHIISRHPDSDLNPILAVGNCTLNLLSKEGKRQIPLNEQFLSKCPNADLKPQEILVSVNIPYSRK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Can you please provide the following:
1)For catalog: CSB-EP001858HU (all expression systems):
a.Has the tag been removed successfully for any expression systems? If yes, which ones?; b.Do you have any metabolism studies related data? Or knowledge on using this protein in metabolism studies? What co-factors are involved? What positive control can I use? (I don’t expect you to know this Vivian but thought I’d ask just in case you may.)
2)I am also looking for the following isoforms you may have, 2,3,4,etc. :
a.Recombinant Human Aldehyde oxidase 2 (full-length); b.Recombinant Human Aldehyde oxidase 3 (full-length); c.Recombinant Human Aldehyde oxidase 4 (full-length)
If you have these proteins, can you please also provide the same information requested above for AO a.Sequence; b.Tag type; c.Tag position; d.Sizes and formats; e.Has the tag been removed successfully for any expression systems? If yes, which ones?; f.Do you have any metabolism studies related data?...

A:
Thanks for your inquiry.
Recombinant human Aldehyde oxidase（AOX1）
CSB-YP001858HU >> Yeast
CSB-EP001858HU >> E.coli
CSB-BP001858HU >> Baculovirus
CSB-MP001858HU >> Mammalian cell
Sorry, we have never removed the tag of this protein before, We can try enzyme digestion, but we can't guarantee 100% successfully.
The overall success rate of enzyme digestion data analysis is 75%-86%, but we should explaing to you in advance:
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge for extra cost.
If we fail in removing the tag, we won’t charge for tag removal and provide the fusion protein, and remark this information in datasheet as follows “Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
Generally, the delivery time will be extended for 3 days.
Please refer to the following link, it have the information of the metabolism studies: http://dmd.aspetjournals.org/content/38/8/1322.long
About the Aldehyde oxidase, the human species only express AOX1, so we don't have 2,3,4.

靶点详情

  • 功能：
    Oxidase with broad substrate specificity, oxidizing aromatic azaheterocycles, such as N1-methylnicotinamide, N-methylphthalazinium and phthalazine, as well as aldehydes, such as benzaldehyde, retinal, pyridoxal, and vanillin. Plays a key role in the metabolism of xenobiotics and drugs containing aromatic azaheterocyclic substituents. Participates in the bioactivation of prodrugs such as famciclovir, catalyzing the oxidation step from 6-deoxypenciclovir to penciclovir, which is a potent antiviral agent. Is probably involved in the regulation of reactive oxygen species homeostasis. May be a prominent source of superoxide generation via the one-electron reduction of molecular oxygen. Also may catalyze nitric oxide (NO) production via the reduction of nitrite to NO with NADH or aldehyde as electron donor. May play a role in adipogenesis.
  • 基因功能参考文献：
    1. AOX1 SNPs exist in which the base-pair exchange of the SNP leads to loss of enzyme activity. PMID: 26842593
    2. ten novel single nucleotide polymorphisms resulting in amino acid exchanges in proximity to the FAD site of hAOX1, were characterized. PMID: 28750088
    3. Data suggest that substrate specificity of AOX1 includes GDC-0834, an inhibitor of Bruton's tyrosine kinase with potential non-steroidal anti-inflammatory agent activity; in liver cytosol, hydrolysis of GDC-0834 is primarily due to AOX1. PMID: 25845827
    4. Methotrexate is poorly metabolized by human AOX1, in contrast to rabbit. PMID: 26032640
    5. These results supported the possibility that XO/XD and AO can contribute to nitric oxide generation. PMID: 25537183
    6. Several donors have a normal AOX1 protein level. PMID: 23857892
    7. High AOX1 methylation was associated with biochemical recurrence in prostate cancer. PMID: 23918943
    8. The physiological role of AOX1 remains unclear, but it is known that thiopurines belong to its substrates; AOX1 contributes to azathioprine catabolism. PMID: 22495427
    9. hydralazine, at a concentration of 25 to 50 muM, can be used in human hepatocyte incubations to estimate the contribution of aldehyde oxidase to the hepatic clearance of drugs and other compounds. PMID: 22522748
    10. Human population is characterized by the presence of functionally inactive hAOX1 allelic variants as well as variants encoding enzymes with different catalytic activities. PMID: 22279051
    11. characterization of AO activity in cryopreserved human hepatocytes: enzyme stability; substrate specificity; kinetics; inhibition by hydralazine PMID: 22031625
    12. Expressed AOX1 in Escherichia coli and purified kinetically active protein. PMID: 19741035

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Xanthine dehydrogenase family
  • 组织特异性：
    Abundant in liver, expressed in adipose tissue and at lower levels in lung, skeletal muscle, pancreas. In contrast to mice, no significant gender difference in AOX1 expression level (at protein level).
  • 数据库链接：

    HGNC: 553

    OMIM: 602841

    KEGG: hsa:316

    STRING: 9606.ENSP00000363832

    UniGene: Hs.406238