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  4. Recombinant Mouse Interleukin-21 protein (Il21)

Recombinant Mouse Interleukin-21 protein (Il21)

In Stock
  • 货号：
    CSB-AP003391MO
  • 规格：
    ￥2124
  • 图片：
  • 其他：

产品详情

  • 纯度：
    >98% as determined by SDS-PAGE.
  • 内毒素：
    Less than 1.0 EU/μg as determined by LAL method.
  • 生物活性：
    Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 T cells is less than 25 ng/ml, corresponding to a specific activity of >4.0x104 IU/mg.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Il21Interleukin-21; IL-21
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.Coli
  • 分子量：
    15.0 kDa
  • 表达区域：
    18-146aa
  • 氨基酸序列
    HKSSPQGPDR LLIRLRHLID IVEQLKIYEN DLDPELLSAP QDVKGHCEHA AFACFQKAKL KPSNPGNNKT FIIDLVAQLR RRLPARRGGK KQKHIAKCPS CDSYEKRTPK EFLERLKWLL QKMIHQHLS
  • 蛋白标签：
    Tag-Free
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    0.2 m filtered 2 x PBS, pH 7.4 ,lyophilized
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    5-10 business days
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
  • 基因功能参考文献：
    1. Injection of IL-21-expressing or IL-33-expressing plasmids facilitates clearance of pre-established genotype B strain designated BPS (BPS) persistence and protects cured mice from BPS re-challenge. PMID: 29242561
    2. this study shows that IL4 and IL21 cooperate to induce the high Bcl6 protein level required for germinal center formation PMID: 28875978
    3. This finding warrants future development of IL-21 and IL-7 co-expressing whole-cell cancer vaccines and their relevant combinatorial regimens. PMID: 27571893
    4. The studies link IL-21 to T cell ontogeny, self-reactivity, and humoral autoimmunity. PMID: 28978474
    5. IL-21 signaling has a critical role in optimal host resistance against Mycobacterium tuberculosis infection PMID: 27819295
    6. memory-like NK cells expansion depends on IL-21, and is involved in vaccine-induced protective immunity against a bacterial pathogen PMID: 27924822
    7. IL-6 is identified as a master regulator of IL-21 in effector CD8(+) T cells. PMID: 27670591
    8. Our work suggests that IL-21 regulates megakaryocyte development and platelet homeostasis. Thus, IL-21 may link immune responses to physiological or pathological platelet-dependent processes. PMID: 28057742
    9. we identify novel distal regulatory elements in the mouse Il21 locus and demonstrate a dynamic long-range chromatin interaction between the Il21 promoter and these regulatory elements, which contributes to the regulated expression of IL-21 PMID: 27067007
    10. this study shows that IL-21 acts as a potent inhibitor of a IL-17A-producing T-cell subset during Mycobacterium bovis Bacillus infection PMID: 27554052
    11. this study shows that combination of mIL-21 with HIV-1 vaccines leads to distinct enhancement of NK cells and T cell immune responses associated with immune protection PMID: 27062692
    12. The data of this study suggested that an increase in astrocyte-derived IL-21 expression in hippocampal subregions following KA-induced seizures may have potent regulatory effects on epileptogenesis. PMID: 27118610
    13. this study reveals a context-dependent role for IL-21 in sustaining effector phenotype CD8 T cells and influencing their migratory properties, accumulation, and functions. PMID: 26826252
    14. Dysregulated development of IL-17- and IL-21-expressing follicular helper T cells and increased germinal center formation in the absence of RORgammat. PMID: 26499265
    15. results demonstrate that, in the setting of lupus-like CD4 T cell-driven B cell hyperactivity, IL-21 signaling on Ag-specific donor CD8 T cells is critical for CTL effector maturation whereas a lack of IL-21R downregulates CTL responses that would otherwise limit B cell hyperactivity and autoantibody production PMID: 26792801
    16. IL-21 from microbiota-specific Th17 and/or Tfh cells contributes to robust intestinal IgA levels by enhancing IgA(+) CSR, IgA production and B-cell trafficking into the intestine. PMID: 25586558
    17. this report demonstrates a critical role for IL-21 in the generation of a primary effector CD8 T cell response to an infectious disease model. PMID: 26597007
    18. IL-21 drives the activation and expansion of a novel population of IL-13-producing Tc2 cells, which serve as a critical link between inflammation and fibrosis. PMID: 26519529
    19. these findings underscore the important collaboration between IL-4 and IL-21 in shaping T-dependent B cells antibody responses. PMID: 26491200
    20. IL-21-mediated signaling supports generation and stabilization of pathogenic Th17 cells and development of spontaneous autoimmunity. PMID: 26413871
    21. In vivo treatment with IL-21 results in complete mantle cell lymphoma regression in syngeneic mice via natural killer - and T-cell-dependent mechanisms. PMID: 26194763
    22. genetic deficiency results in reduced Th2 type response during allergic airway inflammation PMID: 26287681
    23. Data indicate that interleukin 21 knockout (il-21-/-) mice showed significantly diminished T follicular helper cell (TFH) generation/accumulation compared to wild type mice. PMID: 25251568
    24. The results indirectly provide evidence that IL-21 produced by CD4(+) T cells could promote Th1/Tc1 response, leading to systemic inflammation in emphysema. PMID: 24357415
    25. IL-21 suppresses the development of Th2 cells and functions of polarized Th2 cells. PMID: 25351608
    26. Inflammation and lymphopenia trigger autoimmunity by suppression of IL-2-controlled regulatory T cell and increase of IL-21-mediated effector T cell expansion. PMID: 25339665
    27. IL-21 blockage decreases BAFF levels, modulates T-cell function in acute GVHD, attenuates GVHD by inhibiting mTORC, and modulates B-cell homeostasis. PMID: 25246268
    28. In comparison with the DC-hgp100 or mIL-21 groups, the combinational DC-hgp100/mIL-21 vaccine drastically suppressed the myeloid-derived suppressor cells (MDSCs) and T-regulatory (Treg) cell populations. PMID: 24572790
    29. Data suggest that IL-21 exerts negative regulation on IRF4 and Treg activity, developing and maintaining adipose tissue inflammation in the obesity state. PMID: 24430438
    30. Under IL-2 deprivation conditions, IL-21 may act as the major survival factor promoting T cell immune responses. PMID: 24416451
    31. GM-SCF, IL-21 and Rae1 expression, alone or in combination, induces a cellular immune response against H22 tumor cells. PMID: 24350772
    32. IL-21 is highly up-regulated in the injured mouse brain after cerebral ischemia. PMID: 24616379
    33. Opposing actions of IL-2 and IL-21 on Th9 differentiation correlate with their differential regulation of BCL6 expression. PMID: 24550509
    34. CD86 expression has functional consequences for the magnitude of CD4 T cell responses both in vitro and in vivo. These data pinpoint CD86 upregulation as an additional mechanism by which IL-21 can elicit immunomodulatory effects. PMID: 24470500
    35. interleukin-21 enhances rituximab activity PMID: 23825648
    36. results indicate that besides IL-21, IL-21iso may be another regulator of gut inflammation PMID: 23557800
    37. IL-21 signaling to B cells is essential for the development of all classical disease manifestations, but IL-21 signaling also supports the expansion of central memory, CD8(+) suppressor cells and broadly represses the cytokine activity of CD4(+) T cells. PMID: 24078696
    38. IL-21 may also indirectly promote CD8+ T cell poly-functionality by restricting the suppressive activity of infection-induced Treg cells. PMID: 23696736
    39. a key role for IL-21 in shaping the humoral and cellular response to T. gondii PMID: 23667536
    40. IL-21 plays a critical role in regulating central nervous system immunity during viral encephalitis by sustaining viral persistence and preventing mortality. PMID: 23992866
    41. REsults suggest IL-21 is not required for follicular helper t-cell differentiation and function and IL-21 must act on B cells PMID: 23960240
    42. Notably, Th17 differentiation in the absence of NR4A2 was restored by exogenous IL-21, indicating that NR4A2 controls full maturation of Th17 cells via autocrine IL-21 signalling PMID: 23437182
    43. required for induction of type I diabetes PMID: 23607664
    44. These preliminary data show that IL-21 is critical for the development of optimal vaccine-induced primary but not secondary antibody responses against rabies virus infections. PMID: 23516660
    45. Interleukin-21 is a critical cytokine for the generation of virus-specific long-lived plasma cells. PMID: 23637417
    46. Secreted IL-21 isoform also has less signaling activity than IL-21. PMID: 23312859
    47. IL-21 mRNA is reduced in the spleens of lyn(-/-) IL-6(-/-) and lyn(-/-) Btk(lo) mice. PMID: 23169140
    48. Together, these results showed for the first time that IL-21 influences the biology and the effector activity of Natural killer dendritic cells. PMID: 23098768
    49. Local suppression of IL-21 alleviated secretory disfunction and disrupted lymphocyte infiltration in submandibular glands of NOD mice and thus retarded the development of Sjorgren's-like symptoms of NOD mice. Tfh cells were also inhibited. PMID: 22643047
    50. Results indicate a reciprocal production of IL-21 and GM-CSF in T4 cells. PMID: 23453633

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-15/IL-21 family
  • 组织特异性：
    Expressed in CD4(+) T cells.
  • 数据库链接：

    KEGG: mmu:60505

    STRING: 10090.ENSMUSP00000029273

    UniGene: Mm.157689