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  4. Recombinant Mouse Leukocyte surface antigen CD47 (Cd47), partial (Active)

Recombinant Mouse Leukocyte surface antigen CD47 (Cd47), partial (Active)

Unavailable
  • 货号：
    CSB-AP005351MO
  • 规格：
    ￥1104
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 95% as determined by SDS-PAGE.
  • 内毒素：
    Less than 1.0 EU/μg as determined by LAL method.
  • 生物活性：
    The ED50 as determined by its ability to bind Mouse SIRPA in functional ELISA is less than 20 ug/ml.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cd47; Leukocyte surface antigen CD47; Integrin-associated protein; IAP; CD antigen CD47
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial of Isoform 2
  • 来源：
    Mammalian cell
  • 分子量：
    16.7 kDa
  • 表达区域：
    19-158aa
  • 氨基酸序列
    QLLFSNVNSIEFTSCNETVVIPCIVRNVEAQSTEEMFVKWKLNKSYIFIYDGNKNSTTTDQNFTSAKISVSDLINGIASLKMDKRDAMVGNYTCEVTELSREGKTVIELKNRTAFNTDQGSACSYEEEKGGCKLVSWFSP
  • 蛋白标签：
    C-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from a 0.2 μm filtered 1xPBS, pH 7.4
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
  • 基因功能参考文献：
    1. this study shows that inhibition of IAP represses inflammatory status via nuclear factor-kappa B pathway in murine endometriosis lesions PMID: 29105884
    2. Our results support an intrinsic role of CD47 in ovarian cancer progression and immune evasion PMID: 28380460
    3. mice deficient in CD47 (CD47 Knockout) had significantly less brain neutrophil infiltration at 24h, upregulated VEGF expression in peri-lesion cortex at 7 and 14day PMID: 27931776
    4. TSP1 significantly accelerates replicative senescence and associated cell cycle arrest in a CD47-dependent manner. PMID: 27607583
    5. CD47, TSP1, and to a lesser extent SIRPalpha facilitate exosome-mediated myeloid-derived suppressor cells chemotaxis and migration. PMID: 27728760
    6. In pulmonary hypertension TSP1-CD47 is upregulated, and contributes to pulmonary arterial vasculopathy and dysfunction. PMID: 27742621
    7. thrombospondin-1 via CD47 inhibits renal tubular epithelial cell recovery after ischemia reperfusion injury through inhibition of proliferation/self-renewal. PMID: 27259369
    8. these findings have demonstrated how tumor cells inhibit innate sensing in dendritic cells and suggested that the CD47-SIRPalpha axis is critical for dendritic cell-driven antitumor immunity PMID: 28801234
    9. this study shows that CD47 deficiency in tumor stroma promotes tumor progression by enhancing angiogenesis PMID: 27283989
    10. the results obtained by combining bioinformatics and preclinical studies strongly suggest that targeting TSP-1/CD47 axis may represent a valuable therapeutic alternative for hampering melanoma spreading. PMID: 27349907
    11. Treg cells protect dopaminergic neurons against MPP+ neurotoxicity by a cell-to-cell contact mechanism underlying CD47-SIRPA interaction and Rac1/Akt activation. PMID: 28268219
    12. CD47 deficient mice with vaccination showed greater protective efficacy against lethal challenge. PMID: 27194758
    13. These results indicate an important role for CD47-SIRPalpha interactions in innate control of malaria and suggest novel targets for intervention. PMID: 27091932
    14. atherogenesis is associated with upregulation of CD47, a key anti-phagocytic molecule that is known to render malignant cells resistant to programmed cell removal, or 'efferocytosis' PMID: 27437576
    15. Erythrocyte CD47 has a key role in hematoma clearance after intracerebral hemorrhage. PMID: 26732568
    16. These data indicate that CD47 plays protective roles against disseminated candidiasis and alters pro-inflammatory and immunosuppressive pathways known to regulate innate and T cell immunity. PMID: 26010544
    17. CD47 deficiency ameliorates lupus nephritis in Fas(lpr) mice via suppression of IgG autoantibody production. PMID: 26095930
    18. the cell adhesion molecule CD47 participates in multiple phases of granule cell development, including proliferation, migration, and neurite differentiation PMID: 25288019
    19. CD47 mediates signaling from the extracellular matrix that coordinately regulates basal metabolism and cytoprotective responses to radiation injury PMID: 26311851
    20. Taken together, these data revealed a novel role for CD47 in the development of obesity and its related metabolic complications. PMID: 25747123
    21. NF KB mediated CD47 up regulation promotes sorafenib resistance treating hepatocellular carcinoma. PMID: 25902734
    22. Loss of cell surface CD47 clustering formation and binding avidity to SIRPalpha facilitate apoptotic cell clearance by macrophages. PMID: 26085683
    23. findings prove that the TSP-1/CD47/SIRP-alpha signal axis is important to the evolution of tumor cells in the microenvironment of immunotherapy and identify thrombospondin-1 as a key signal PMID: 25697354
    24. Thrombospondin-1 and CD47 signaling regulate healing of thermal injury in mice. PMID: 24840925
    25. the microglial proinflammatory response to Abeta(1-42) protofibril is not dependent on CD47 PMID: 25451248
    26. CD47 expression in the microenvironment was sufficient to limit tumor radiosensitivity. PMID: 25297630
    27. role in promoting left ventricular heart failure by CaKMII-mediated up-regulation of HDAC3 PMID: 24922625
    28. Polymorphism in the innate immune receptor SIRPalpha controls CD47 binding and autoimmunity in the nonobese diabetic mouse. PMID: 25305319
    29. using a syngeneic mouse hepatocyte transplantation model, the present study demonstrates that missing CD47 on donor cells alone can cause recipient myeloid cell activation and graft loss. PMID: 23394628
    30. sought to investigate the effect of Hsp70-peptide complex on the expression of CD172alpha and CD47 receptors in normal peritoneal macrophages (NMO) PMID: 24684700
    31. CD47 plays the pivotal role in the immune evasion of primary effusion lymphoma cells in body cavities. Therapeutic antibody targeting of CD47 could be an effective therapy for PEL. PMID: 24726056
    32. 'clustering' SIRPalpha into plasma membrane microdomains is essential for activated monocytes and macrophages to effectively interact with CD47 and initiate intracellular signaling PMID: 24143245
    33. Thrombospondin-1 signaling through CD47 is the first identified endogenous inhibitor of H2S signaling and constitutes a novel mechanism that negatively regulates T cell activation. PMID: 23499828
    34. Data indicate that lack of CD47 strongly impairs SIRPalpha-dependent osteoblast differentiation, deteriorate bone formation, and cause reduced formation of osteoclasts. PMID: 23990469
    35. Thus, CD47 antagonists enable cell self-renewal and reprogramming by overcoming negative regulation of c-Myc and other stem cell transcription factors. PMID: 23591719
    36. CD47 deficiency confers cell survival through the activation of autophagic flux and identifies CD47 blockade as a pharmacological route to modulate autophagy for protecting tissue from radiation injury. PMID: 22874555
    37. Inhibitor of apoptosis proteins (IAPs) and their antagonists regulate spontaneous and tumor necrosis factor (TNF)-induced proinflammatory cytokine and chemokine production PMID: 23275336
    38. CD47 deficiency promotes nerve fiber growth from cultured ventral mesencephalic dopamine neurons. PMID: 23049778
    39. Although polymorphonuclear neutrophil (PMN) transmigration is not delayed in CD47-deficient mice, fewer neutrophils are found in the intestine at the postacute/chronic stage of chronic colitis. PMID: 23203922
    40. CD47(low) status on CD4 effectors is necessary for the contraction/resolution of the immune response in humans and mice. PMID: 22870271
    41. Thrombospondin-1 regulates blood flow via CD47 receptor-mediated activation of NADPH oxidase 1. PMID: 23087362
    42. ectopic expression of murine Cd47 in human hepatocytes selectively favors engraftment upon transplantation into mice, a finding that should have a profound impact on the generation of robust humanized small animal models. PMID: 22535707
    43. Activation of parenchymal CD47 promotes renal ischemia-reperfusion injury. PMID: 22859854
    44. Engagement of endothelial CD47 by its ligands triggers outside-in signals in endothelium, facilitating leukocyte transendothelial migration at sites of inflammation. PMID: 22815286
    45. Data show that CD47(-/-) mice are refractory to experimental autoimmune encephalomyelitis (EAE). PMID: 22734047
    46. Activated CD47 promotes pulmonary arterial hypertension through targeting caveolin-1. PMID: 22215724
    47. CD47(high) status on CD4 T cells identifies functional long-lived memory T cell progenitors. PMID: 22461697
    48. CD47 signalling is dispensable for oral tolerance induction, whereas the expression of CD47 by non-haematopoietic cells is required for intestinal IgA B-cell responses. PMID: 22070457
    49. provides a rational basis for targeting CD47-SIRPalpha interactions, using for instance the antagonistic antibodies against human SIRPalpha described herein, to potentiate the clinical effects of cancer therapeutic antibodies PMID: 22042861
    50. CD47 deletion improves functional recovery from spinal cord injury via an increase in vascular patency, functional locomotor improvements and greater white matter sparing. PMID: 21168495

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 数据库链接：

    KEGG: mmu:16423

    UniGene: Mm.31752