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  4. Recombinant Human Growth/differentiation factor 5 (GDF5)

Recombinant Human Growth/differentiation factor 5 (GDF5)

In Stock
  • 货号：
    CSB-AP005981HU
  • 规格：
    ￥852
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    > 95 % as determined by SDS-PAGE
  • 内毒素：
    Less than 0.1 EU/ug as determined by LAL method.
  • 生物活性：
    Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg.
  • 基因名：
  • Uniprot No.：
  • 别名：
    BMP14; Cartilage derived morphogenetic protein 1; Cartilage-derived morphogenetic protein 1; CDMP-1; CDMP1; GDF-5; Gdf5; GDF5_HUMAN; Growth differentiation factor 5; Growth/differentiation factor 5; LAP4; OS5; Radotermin; SYNS2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.Coli
  • 分子量：
    13.6 kDa
  • 表达区域：
    382-501aa
  • 氨基酸序列
    APLATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR
  • 蛋白标签：
    Tag-Free
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    5-10 business days
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction. Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG. Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes.
  • 基因功能参考文献：
    1. We found a strong association between the TT genotype and the risk of developing Knee Osteoarthritis (OR = 1.7, 95% CI = 1.12-2.8, p = 0.014), but not in the heterozygous TC state (OR = 1.56, CI 95% = 0.58-4.17, p = 0.367). PMID: 30044130
    2. study shows that there exists a relationship between GDF5 (SNP rs143383) and Developmental dysplasia of the hip (DDH) in our population. Second, we found for the first time that the genotype TT and the T allele were overly expressed in the patients and the fathers. More studies on the confirmation of this genetic marker for DDH are called for. PMID: 29797005
    3. Two Pakistani families with sequence variants in GDF5 and TRPS1 causing brachydactyly type C and tricho-rhino-phalangeal syndrome type III are described. PMID: 29436063
    4. The dysfunctional gene GDF5 was successfully corrected in adipose tissue-derived mesenchymal stem cells using a pair of transcription activatorlike effector nucleases. PMID: 29393424
    5. No association has been found between GDF5 +104 T/C promoter polymorphism and osteoarthritis in the Eastern Turkey population. PMID: 28886316
    6. The results of the current study revealed that SNP rs143383 of GDF5 is a compelling risk factor for knee OA [Osteoarthritis] and that GDF5 has an etiological effect on the development of OA [Osteoarthritis]. PMID: 29056119
    7. A Study of IL-1beta, MMP-3, TGF-beta1, and GDF5 Polymorphisms and Their Association with Primary Frozen Shoulder in a Chinese Han Population PMID: 28676856
    8. BMP-14 rs143383 polymorphism reduced the susceptibility to knee osteoarthritis (OA) and hand OA not only in total analysis but also in subgroup analysis; BMP-14 rs143383 polymorphism may be a protective factor against OA occurrence PMID: 29049177
    9. The structure of Grem2-GDF5 complex has revealed a number of key findings for DAN-family mediated BMP2 inhibition. PMID: 27524626
    10. miR-615-3p negatively regulates the osteogenic differentiation of hLF cells through post-transcriptionally suppressing osteogenic regulators GDF5 and FOXO1. PMID: 28460412
    11. p38, c-jun, and NFkappaB pathways activated during intervertebral disc degeneration by IL-1beta but not GDF-5 PMID: 27391542
    12. GDF5 elicited significant (p < 0.05) changes in the expression of anabolic, catabolic and hypertrophic genes with several consistent effects in healthy donors and in OA patients PMID: 28481944
    13. GDF5 was up regulated in patients after chronic rhinosinusitis developing osteitis. PMID: 27888647
    14. Titanium (Ti) surface modification with the combination of hBMP-2 and hGDF-5 for the two growth factor-coated Ti implants can improve the clinical properties of implants for orthopedic and dental applications. PMID: 28124978
    15. he large array of modular enhancers for Gdf5 provide a new foundation for studying the spatial specificity of joint patterning in vertebrates, as well as new candidates for regulatory regions that may also influence osteoarthritis risk in human population PMID: 27902701
    16. The purpose of this study is to investigate the immunohistochemical expression of cytokeratin 18 (CK18) and the reactivity to GDF5 (CDMP-1), called the morphogenetic protein-1, cartilage-derived, in lingual squamous cell carcinoma. PMID: 27151703
    17. homozygous sequence variants in the GDF5 gene underlie acromesomelic dysplasia type-grebe in consanguineous families PMID: 27577507
    18. The prevention of IL-1Beta-induced nucleus pulposus extracellular matrix degeneration by miR-7 silencing was attenuated by GDF5 siRNA. PMID: 27583982
    19. Mutations in three genes (GDF5, NPR2, BMPR1B) have been reported to cause different forms of acromesomelic dysplasia PMID: 26926249
    20. we demonstrate that the transforming growth factor-beta1 and the growth differentiation factor 5 synergistically drive the nucleopulpogenic differentiation process. The commitment of the hASCs was robust and highly specific as attested by the expression of NP-related genes characteristic of young healthy human NP cells PMID: 26661057
    21. The data suggest that Ad-GDF-5 gene therapy is a potential treatment for IDD, which restores the functions of degenerative intervertebral disc through enhancing the ECM production of human NP cells. PMID: 26739524
    22. An association of SNP in GDF5 with temporomandibular joint osteoarthritis in female Han Chinese. PMID: 25757091
    23. results demonstrate that SNP rs143383 of GDF5 is a compelling risk factor for both knee and hand osteoarthritis (OA) and provide further support for GDF5 in the etiology of OA [meta-analysis] PMID: 25894512
    24. Two novel homozygous missense mutations in the GDF5 gene cause brachydactyly type C. PMID: 25820810
    25. our results showed that GDF-5 and BMPRII expressed both in normal and degenerated intervertebral disc tissues, and GDF-5 might have an inhibition effect on degenerated lumbar intervertebral discs PMID: 25755766
    26. This meta-analysis finds that the C allele and CC genotype of the GDF5 gene are protective for knee osteoarthritis susceptibility. PMID: 25467786
    27. Our results revealed that the GDF5 SNP was associated with susceptibility to the meniscus injury and postoperative function recovery in Chinese male soldiers. PMID: 24227118
    28. missense mutations p.T201P and p.L263P interfere with the protein structure and thereby reduce the amount of fully processed, biologically active GDF5, finally causing the clinical loss of function phenotype. PMID: 25092592
    29. The proregion is stabilized by an intramolecular disulfide bond. The isolated proregion folds independently of the mature domain. PMID: 25174448
    30. Growth differentiation factor 5 and canonical Wnt signaling may contribute to molecular mechanisms of osteoarthritis. PMID: 24561281
    31. These results suggest that obesity leads to upregulation of GDF5 expression responsible for the promotion of brown adipogenesis through a mechanism relevant to activation of the NF-kappaB pathway. PMID: 25223801
    32. results suggested that GDF5 polymorphism is associated with susceptibility to symptomatic lumbar disc herniation in Chinese Han population and type II collagen in the nucleus pulposus may be a factor in susceptibility to symptomatic lumbar disc herniation PMID: 24105021
    33. Osteoarthritis chondrocytes do not respond in a predictable manner to culture with exogenous GDF5. PMID: 24466161
    34. High GDF5 expression is associated with osteoarthritis. PMID: 24861163
    35. The expression of growth differentiation factor 5 (GDF5) and aggrecan in 15 cases of salivary gland pleomorphic adenomas, was investigated. PMID: 24398992
    36. established an association between two SNPs (rs224332 and rs224333) of GDF5 and DDH development in a female Chinese population. PMID: 24114442
    37. In vitro findings suggest that the degenerating disc milieu, with high proinflammatory cytokine levels, may limit expression of GDF-5, resulting in limited regenerative capacity of the intact disc. PMID: 24582800
    38. These novel insights into the biology of GDF5 might also provide further clues on the pathophysiology of OA. PMID: 24098149
    39. The novel missense mutation p.Leu176Pro causes impaired secretion of GDF5 in Brachydactyly type C and mild Grebe type chondrodyslplasia. PMID: 23812741
    40. GDF5 is the only osteoarthritis susceptibility gene so far identified with definite evidence.[Review] PMID: 24003854
    41. Overall, a statistically significant association was found between the +104T/C polymorphism of GDF5 and risk of knee osteoarthritis PMID: 23151597
    42. GDF5 harbors a C/A transversion located -41 bp relative to the transcription start site that leads to increased gene expression. PMID: 22929025
    43. GDF5 polymorphisms are associated with susceptibility to low back pain during military training in Chinese soldiers. PMID: 23725396
    44. In conclusion, the rs143383 variant was not found to associate with the risk of ACL rupture. PMID: 23090674
    45. we have identified four trans-acting factors that are binding to GDF5, three of which are modulating GDF5 expression via the OA susceptibility locus rs143383. PMID: 23825960
    46. Although the effect size of the association between OA and GDF5 is small, there is suggestive evidence for an association. PMID: 23423687
    47. GDF5 regulates TGF-beta-dependent angiogenesis in breast carcinoma cells. PMID: 23226264
    48. Growth differentiation factor 5 modulation of chondrogenesis of self-assembled constructs involves gap junction-mediated intercellular communication. PMID: 23121099
    49. analysis of positive selection on the osteoarthritis-risk and decreased-height associated variants at the GDF5 gene in East Asians PMID: 22905146
    50. Our findings in 5 population cohorts from Northern Europe indicate that a variant in the GDF5 gene is a risk factor for lumbar disc degeneration in women. PMID: 21360499

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  • 相关疾病：
    Acromesomelic chondrodysplasia, Grebe type (AMDG); Acromesomelic chondrodysplasia, Hunter-Thompson type (AMDH); Brachydactyly C (BDC); Du Pan syndrome (DPS); Symphalangism, proximal 1B (SYM1B); Multiple synostoses syndrome 2 (SYNS2); Brachydactyly A2 (BDA2); Osteoarthritis 5 (OS5); Brachydactyly A1, C (BDA1C)
  • 亚细胞定位：
    Secreted. Cell membrane.
  • 蛋白家族：
    TGF-beta family
  • 组织特异性：
    Predominantly expressed in long bones during embryonic development. Expressed in monocytes (at protein level).
  • 数据库链接：

    HGNC: 4220

    OMIM: 112600

    KEGG: hsa:8200

    STRING: 9606.ENSP00000363489

    UniGene: Hs.1573